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Endocrine disruptors induce transgenerational alterations of the male reproductive parameters and mirna expression profiles in mouse primordial germ cells
Děd, Lukáš ; Brieno-Enríquez, M.A. ; García-López, J. ; Cárdenas, D.B. ; Guibert, S. ; Hourcade, J.de D. ; Pěknicová, Jana ; Weber, M. ; del Mazo, J.
Primordial germ cells (PGCs) are the embryonic precursors of the germ cell linage, which are restricted to form only sperm and oocytes following their specification from pluripotent cells. PGC precursors are specified in the epiblast around 6.25 days post coitum (dpc), and around 7.25 dpc become identifiable in a 40 cell-cluster. In the present study, we used a mouse model to evaluate the trans-generational (F1-F3) effects of vinclozolin (VCZ) administrated in two doses on male reproductive parameters. We observed decreased fertility rate, higher apoptotic rate and histopathologic alterations in adult testis, PGC number reduction, increments of PGCs apoptosis and changes in PGCs gene expression among all three generations. In the attempt to clarify the trans-generational transmition of the altered phenotypes, we performed the microRNA expression and DNA methylation analysis. We observed the significant alteration in the expression of multiple microRNA and microRNA-regulated genes which are important for PGCs specification, including LIN28, let-7 and BLIMP1. Trans-generational deregulation in the expression of factors involved in the Lin28-let-7-Blimp1 pathway can lead to specific VCZ-induced phenotype observed in our study.
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Vliv klíštěcích slin na žírné buňky na úrovni signálních drah
HEJDOVÁ, Barbora
Intracellular signalling molecules create the signalling cascades which enable the transfer of the signal to the cell. In this work we have studied the influence of tick saliva on the cytokine production and the activation of signalling pathways in ionomycin stimulated murine mast cells. We found out that tick saliva inhibits production of several cytokines and affects two important signalling pathways in mast cells possibly involved in the regulation of cytokine induction.
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Perspectives of Transatlantic Trade and Investment Partnership: benefits and potential risks
Bednář, Milan ; Ševčíková, Michaela (advisor) ; Zamrazilová, Eva (referee)
It is possible that the economic condition of Europe after the global financial crisis of 2008-2009 prompted idea of trade liberalization. This diploma thesis deals with the Transatlantic Trade and Investment Partnership (TTIP), a proposed free trade agreement between the European Union and the United States of America. Main goal of the thesis is to provide economic evaluation of the deal, and to assess the claim that TTIP would be beneficial for the European Union member states. Main used method is a theoretical analysis supplemented by regression analysis. The theoretical part is focused on basic economic principles of international trade and related concepts. Main tools used to assess this agreement are described in more detail as well. The analytical part deals with economic linkages between the two regions, with emphasis on the development and current status of non-tariff barriers to trade. The thesis also contains a summary of potential benefits and risks. Finally, a potential impact of TTIP on the Czech Republic and the issue of Brexit is presented. The European Union and the United States of America are linked by strong economic ties. However, trade barriers between those two entities still exist and hamper international trade. The analysis indicates that if the contract was to be impactful and significant, it must focus on a substantial reduction of bilateral non-tariff barriers to trade. This implies that TTIP could interfere with sectoral regulations. In addition, it is not certain that achieved revenues would be automatically higher than costs given the number of perceived risks. Panel data gravity models are used to quantify the potential impact of trade liberalization on export of goods of the EU28 countries to the USA. Significant elimination of trade barriers could increase EU exports to the USA by more than 20%.
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Inbound marketing of Nestlé in the area of infant formula
Hnízdilová, Lucie ; Průša, Přemysl (advisor) ; Postler, Milan (referee)
This master's thesis deals with inbound marketing of Nestlé in the area of infant formula. Its aim is to determine whether inbound marketing is a suitable tool for infant formula promotion which is strongly regulated. The thesis is based on a comprehensive analysis of the area of infant formula and analysis of inbound marketing strategy of one of the most dominant companies on the market, Nestlé. In the beginning, attention is focused on important milestones of the development of infant formula and on a case which caused an important change in the promotion of infant formula, Nestlé boycott. The following section deals with the supervision and marketing regulation both in international and the European law. The final chapter of the first part emphasizes on philosophy of inbound marketing. The second part is introduced by outlining the current situation both on global and the Czech market of infant formula. Then, attention is focused on Nestlé. Its introduction is followed by analysis of company's inbound marketing strategy which is then evaluated. For this purpose, analysis of the effectiveness of marketing tools is made with regard to strategic goals of the company.
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Explanatory power of financial statements compiled according to czech accounting regulations and IFRS
MAŠKOVÁ, Lenka
The aim of this thesis is to summarize the specifics of reporting under the Czech accounting regulations and International Financial Reporting Standards and through the analysis evaluate the explanatory power of selected accounting items. This thesis consists of two parts. The first part, which is the theoretical one, deals with financial statements, explanatory power, valuation and other major items. In the practical part the financial statements and their items are analyzed and the differences between the Czech accounting regulations and International Financial Reporting Standards are evaluated.
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Health care for employees - the employer's obligations
PŘEDOTOVÁ, Eva
People were aware of the connection between the quality of the job done and the person performing the job as early as in the ancient times and in the times of ancient Egypt. In the course of centuries prevention was favoured over the treatment of an injury or illness. Nowadays the healthcare for employees is regulated by numerous international as well as national rules of law, the most important international one for the observed field being the International Labour Organization Convention No. 161 ? Occupation health services Convention. Upon this international convention ratified in Czechoslovakia in February 1989, many rules of law dealing with the health protection of employees at their workplaces are specified in our legal order, the significant being the Act 262/2006 Coll., Labour Code and newly also the Specific Healthcare Act 373/2011 Coll. The thesis aims to recapitulate the basic rule of law regarding the above-mentioned matter as well as finding out how these rules of law are adhered to and what the employees' awareness concerning their rights and obligations in the area of occupational healthcare is. The practical research of this thesis used qualitative dialogues with the representatives of 10 companies with different business planes. At the same time, a quantitative survey took place among the employees of the same companies. The results indicate that employers meet their obligations to their employees in the area of arranging occupational health services including regular medical checkups. Furthermore, employers try to respect the doctor's capability to work, despite the fact that this area is often quite problematic for them. The employees themselves are aware of their rights and obligations in the area of occupational health services.
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Safe playgrounds in Tabor.
KUNCLOVÁ, Romana
This work deals with the safety of children playgrounds in Tábor. Since 2002 The program of children playground optimisation has been running in the town. Children playgrounds have been reconstructed in the sense of Czech regulations 1176 and 1177 so that they meet requirements of childrens´ safety. The aim of the thesis consists in mapping technical condition of children playgrounds and finding out about public awareness of safe playing.
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The Development Of Company Staff Involvement
PRŮŠOVÁ, Jindřiška
The aim of this work was to evaluate the development of selected staff in a manufacturing concern during the years of 2002 {--} 2005. The company is anonymously marked as "A". There were on the average of 280 workers along tracked period on the premises. The theoretical part deals with relevancy of company staff involvement and monitors points that affects staff behaviour and decision making. The practical part characterizes production workers, managers and administrative staff. It includes analyses of questionnaire inquiry. Interviewees were sampled from the company staff according to their occupations. It was used the statistical method {--} spectrum. It were gained these main findings: - leading factors such as worker´s requirements on improvement of interpersonal relations; growth of remuneration for work; - sense of equally high knowledge rate according to job performance; - agreement with operating company conditions; - there are not uniquely determinated findings in subordination evaluation. Subordinates reproach to approximately one´s half of managers the managerial disabilities, whereas surplus evaluation is opposite.
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Availabity of compensation aids for people with impaired vision after social reform in 2012
KUNEŠOVÁ, Hana
Bachelor thesis "The availability of assistive devices for people with visual impairments after social reform in 2012" discusses specific changes in Act no. 100/1988 Coll., about social security, in particular changes relating to contributions to special tool. The aim of the work is to highlight resulting differences in national regulations and determine whether the interviewed individuals with visual disabilities have changed the approach in selection of assistive devices that they need for their life. Thesis is divided into three parts: theoretical, research, and appendix. The theoretical part focuses on problem of visual impairment and possibility of compensation, because the eye is considered as very important part of every person's life. If a person cannot fully exploit this sense, it is necessary to use various assistive devices to help him cope with everyday situations. However, the individual may use a particular tool, he must meet certain criteria, both in terms of health and finances. Conditions that he must currently meet, so he could be granted a special allowance for aid are set out in Act no. 329/2011 Coll., about provision of benefits for persons with disabilities, which is supplemented by Decree no. 388/2011 Coll., implementing certain provisions of the Act for provision of benefits to persons with disabilities. The above two laws refer to third part of theoretical part, where are precisely defined differences arising just from reform. The research part first sets the goal of work and specific research question, followed by a work methodology that was used in form of qualitative research. Using semi-structured interviews with individual clients of TyfloCentrum in Pilsen I was looking for an answer to research question which is: "Should social reform impact on changing the approach in the selection of mobility aids for persons with visual disabilities?" From respondents' answers the answer to this question revealed, that social reform did not affect the change of approach in selection of compensatory aids in investigation file. These individuals with visual disabilities do not have to re-evaluate their requirements and aids can also choose the same manner as before the reform. Theoretical part of this work may serve as a resource, or as a brief overview of what has by the influence of social reform changed and for what have currently users of assistive devices claim, without tediously search this information on the internet or ask competent authority. Practical part of the work gives the opportunity to use it for services for individual TyfloCenters, where qualitative information can be used to adjust survey offers to its clients, namely withdrawal of tenders of unproven products and vice versa will encourage companies that have good references.
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