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Vliv stájového prostředí na dušnost koní
Trčková, Petra
This thesis focuses on the topic of influence of the stable environment on the shortness of breath of horses. What is today simply called shortness of breath used to be called RAO in the past and nowadays the abbreviation COPD, which stands for Chronic obstructive pulmonary disease, is used. The anatomy of the respiratory system and the physiology of breathing of horses are briefly characterized here. The disease COPD is thoroughly analyzed here together with its etiology, symptomatology, diagnostics, treatment and prognosis. The prevention of diseases in stables and its parameters are addressed here. Research was conducted by means of a questionnaire, which was completed by the owners of both healthy horses and horses suffering from the COPD. The results were evaluated only on the basis of horses affected by COPD.
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Determination of alergenic and potential alergenic metals in cosmetics
Krakovková, Lenka ; Zemanová, Jana (referee) ; Diviš, Pavel (advisor)
The aim of the diploma thesis was to provide an overview of the prevalence of allergenic and potentially allergenic metals in eye shadows. The diploma thesis gives an overview of the legislation on cosmetics and description of the types of allergenic reactions caused by allergenic and potential allergenic metals in eye shadows. Listed below are the preparation methods of the samples for analysis and the list of the methods by which can the selected metals be analyzed. The experimental part of the diploma thesis deals with an analysis of selected allergenic and potentially allergenic metals in eye shadows. In the experimental part of diploma thesis method of sample preparation for analysis of eye shadows and a method of analysis of sample of eye shadows by ICP-MS have been optimized. Monitored analytes were selected metals. Altogether 6 samples of eye shadows from different manufacturers were chosen. Measured results have been statistically processed, confronted with the applicable legislation and assessed in terms of possible allergic reactions.
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Analysis of selected allergens in fragrances
Koláčková, Adéla ; Janoušková, Eva (referee) ; Zemanová, Jana (advisor)
This thesis is focused on potentially allergenic substances (PASs) in cosmetics. Their specific features, methods of control and observation are described. There are also mentioned dermatology problems connected with usage of perfumed cosmetic products in which the allergenic ingredients can be found and possibilities of their regulation. Fragrances are mixtures of natural or synthetic character which give the aroma. In nature, fragrances are produced mainly by plants. More than one hundred fragrances have been identified as allergens. Twenty six of them were declared as PASs. Allergenic components of perfume compositions are the main cause of allergic contact dermatitis. Experimental part of the thesis is focused on the procedure of analysis of specific allergenic fragrances (?-Amylcinnamaldehyde, Anisealcohol, Benzylsalicylate, Cinnamaldehyde, ?-Hexylcinnamaldehyde, 7-Hydroxycitronellal and Methyl-2-octynoate). Headspace-Solid Phase Microextraction connected with Gas Chromatography-Flame Ionisation Detector (HS-SPME-GC-FID) was used for extraction, analysis and identification of these substances. The method was validated and applied on the spectrum of real samples of cosmetic products.
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Analysis of aroma compounds in cosmetics by gas chromatography
Divišová, Radka ; Vítová, Eva (referee) ; Zemanová, Jana (advisor)
This thesis is focused on aroma compounds which are presented in a wide variety of products including perfume, cologne, cosmetics, toiletries, laundry products and detergens. The widespread use and exposure to aroma compounds of aforementioned products could cause a range of the adverse reactions such as contact dermatitis, asthma, eczema and breathing problems. There are a lot of aroma compounds triggering negative allergic reactions but EU regulations limit 26 the most important fragrance allergens. The presence of these substances has to be declared on the product label if a limit of 0,01 % for rinse off and 0,001 % for leave-on products is exceeded. Of these 26 substances, a methodology was elaborated for determination of 12 allergens in a experimental part of this thesis. A method by solid-phase microextraction (SPME) and gas chromatography-flame ionization detection (GC-FID) was applied for fragrance allergens identification, extraction and measurement. The optimal extraction conditions of SPME-GC method were investigated including equilibrium time, extraction time and extraction temperature to increase extraction efficiency. Another important point is the method by SPME-GC-FID was validated and following performance parameters were determined: repeatability, linearity, limit of detection (LOD) limit of quantitation (LOQ). SPME-GC-FID was tested and applied to real samples.
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Monitoring of allergenic aroma compounds in foods
Plevová, Petra ; Sůkalová, Kateřina (referee) ; Vítová, Eva (advisor)
This thesis deals with the identification and quantification of aromatic potentially allergenic substances which normally occurs in food, but their amount is not legislatively controlled. These aroma compounds have many positive and negative effects on human body, although they are not main types of allergens, several studies have highlighted their potentially allergenic character. For monitoring of the content of these substances non-chocolate confectionery were chosen as model samples. Theoretical part summarizes the production technology of non-chocolate confectionery from raw materials to final processing. It also deals with the characterization of 26 potentially allergenic aroma substances and their occurrence in nature and also in food or cosmetic products. In the experimental part potentially allergenic aroma compounds occurring in samples of jelly confectionery were identified and quantified using the SPME–GC–FID method.
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Fragrances of plant origin
Koláčková, Adéla ; Vítová, Eva (referee) ; Zemanová, Jana (advisor)
This thesis describes specifications of fragrances of plant origin, their origin and usage, methods of obtaining and ways of use them in cosmetics. Fragrances are mixtures of natural or synthetic character which give the aroma. In the nature, fragrances are produced mainly by plants. Aroma of blossom and other parts of plants is created by essential oils. From the whole number of 295 families of plants for about a third of them contains essentials oils which can be used in industry. More than 100 fragrances has been identified as allergens. Parfumed products allergies are relatively common. In connection of this problem synthetic alternatives of natural fragrances of plant origin are producing. In this way allergens are and also they will be widely discussed problem.
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Occurence of potential alergenic components with metal content in cosmetic preparations
Krakovková, Lenka ; Janoušková, Eva (referee) ; Zemanová, Jana (advisor)
The aim of the bachelor thesis is to summarize potential allergenic metals contained in cosmetics, make an overview of raw materials used for cosmetics, and describe types of allergenic reactions caused by potential allergenic metals. All the metals presented are divided into two groups, from which one contains established allergens and the other one contains potential allergens, which can have influence on inducing of an allergetic reaction. There is shown a possible allergenic reaction for each metal, and its possible occurence in raw materials for a production of cosmetics, which are discussed in this work as well. There are also shown methods for determining the amount of metals in cosmetics. In the conclusion, there are mentioned some reasons of monitoring potencial allergens from series of metals.
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