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Health care professional as a patient
Uhlířová, Lucie ; Pečenková, Jaroslava (advisor) ; Ulrychová, Hana (referee) ; Švecová, Dagmar (referee)
The bachelor's thesis deals with the topic of a health care professional dealing with personal experience in a patient's role. The theoretical part presents facts associated with health and ilness psychology, as well as pieces of knowledge about human personality and a man's role in human society. It describes the special demands of general nurse's profession on a person. Special attention is paid to a situation when a nurse becomes a patient. The research part analyses the influnce of nurse's profession over her personal health and ilness perception. It describes difficulties that are experienced when a nurse is coping with her own hospital admission. The main goal of the thesis is to find out whether personal experience in a patient's role influences nurse's future life in any way.
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Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Ledvina, Vojtěch ; Klepárník, Karel
Caspases are proteases that play key role in the process of apoptosis, the programmed\ncell death. Among them, caspase-3 and -7 are main executioner caspases that cleave\nmany vital proteins during apoptosis and after their widespread activation, the process\ncannot be reversed. To analyze caspase-3/7 activation within single cells, a miniaturized\ndevice for parallel analysis of eight samples was developed. The assay is based on the\nmodified luciferin-firefly luciferase bioluminescence (BL) system. Individual\nsuspended cells were collected and transferred into detection microvials using a\nmicromanipulator. The bioluminescence was detected using a photon counting head\nwith cooled photcathode. The LOD suitable for detection of active caspase-3/7 in both\napoptotic and non-apoptotic cells was reached.
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Food supplements and iodine supplementation in pregnancy
VAVROUŠKOVÁ, Edita
Iodine is a trace element tremendously important for thyroid gland hormone production. It affects normal evolution of brain, especially in the time of intrauterine development and in the first year of child's life. Deficiency of iodine can lead to creation of pregnancy goiter, it also decrease the probability of carrying fetus to term and increase the probability of disorder in psycho-motoric evolution of child. Czech Republic is geographically lined up among the regions with lack of iodine in natural sources and food. However, nowadays the iodine deficiency is considered to be managed. But risk groups of population still exists, especially pregnant women can be endangered by lack of iodine, because the need of iodine intake in pregnancy increases to 200 250 ug per day and this level must be ensured. Consumption of food rich in iodine can cover the increased need of iodine. Significant source of iodine is milk and milk products. Another source is table salt enriched with iodine, but its daily intake is limited. Rich sources of iodine like sea fish, sea products and seaweed rarely occur in our diet. The goal of thesis was to evaluate intake of dietary supplements with iodine by pregnant women and evaluate women's awareness of higher iodine need and iodine food sources. Quantitative research in form of questionnaires was chosen for getting information from higher number of individuals. The questionnaire consists of 15 questions. Answers were gained from 118 respondents living in Central Bohemia, Moravia-Silesia region, Liberec region, region Highlands and South Bohemia. Questionnaire was distributed to gynecological ambulances by employees of State health institution. I visited gynecological ambulances in South Bohemia personally. By evaluation of questionnaire was found out that dietary supplement with iodine use 63 % pregnant women in researched population. All respondents always use only one type of dietary supplement. Among the most used belong "Chytré miminko", "GS mamavit" and "Femibion 800". 68 % of respondents know the importance of higher iodine intake in time of pregnancy. Question about daily recommended dosage was correctly answered only by 35 % of respondents. As a food sources of iodine the pregnant women know sea fishes, sea products, seaweed and table salt enriched with iodine. But 90 % of respondents are not familiar with milk and milk products as important source of iodine. Pregnant women should be better informed about importance of sufficient supply of iodine and about sources of iodine by gynecological ambulances. Information about mentioned issues should occur in magazines, education materials and internet articles more often. Respondents were notified of issues and important role of iodine in time of pregnancy by questionnaire. It is very probable that women found correct answers on the internet or in publication for expectant mothers after filling in the questionnaire.
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Miřenka Čechová
Janečková, Kateřina ; PETIŠKOVÁ, Ladislava (advisor) ; SOPROVÁ, Jana (referee)
Monographic work focuses on artistic creation Miřenka Čechová, one of the
the leading personalities of our contemporary stagecraft. Her personal and
artistic development started when she began studying dance and continued to
mimic and physical theater. Material presents her contribution to
contemporary Czech scene physical theater and refers to her international
success. Work observes her role like co-founder of physical theaters Theatro
Pantomissimo and Spitfire Company and to presents the work of these
physical theaters and reveals the working and teaching methods in the
preparation of copyright performances.
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The Role of Nurses in Colorectal Surgeries
ŠULCOVÁ, Jana
Theoretical basis: The problems in rectal and anal diseases have had rising tendency, especially haemorrhoidal diseases, on which we put emphasis in our bachelor thesis. 37% of adult population suffers from this disease so we may line it up to civilization illnesses. Their treatment involves wide range of medical methods, from the conservative therapy to semi invasive ways of treatment. The semi invasive treatment is well withstood by patients and it can help up to 90% of clients. This bachelor thesis is concerned with specifics of nursing duties in a colorectal surgery. It links with topics like taking faecal specimen, basic methods of endoscopy, communication with patients and giving them information about lifestyle. The theoretical basis describes haemorrhoidal disease and gives brief introduction into causes of its developing, most common symptoms and possible treatments. The aim of the thesis: From the practical point of view we set two tasks. The first one is aimed at specifics of nursing job in a colorectal surgery and the second one tries to monitor how nurses in colorectal surgeries inform patients about healthy lifestyle. We asked two research questions regarding these aims. The first research question is: What are the specifics of the nursing job in the colorectal surgery? The second question concerns how nurses in colorectal surgeries provide information to their patients about healthy lifestyle. Methodology: Regarding the bachelor thesis we decided to choose a form of a qualitative research with using semi structural interviews with practical nurses working in colorectal surgeries. The interviews took place from February 28 2014 to March 15 2014 in five colorectal surgeries in following towns: České Budějovice, Písek, Strakonice, Jindřichův Hradec a Třemošná u Plzně. The focus group was created of 6 nurses working in colorectal surgeries. The interviews were performed and recorded with the consent of all practical nurses involved in the research. All extracted data was arranged and categorised into groups that were consequently analysed. The interviewer´s questions were prepared in advance and some extra questions were asked during the interview. The interview was divided into five areas: Identification questions, role of a nurse, examination methods, recommendation and giving information about lifestyle. Results: The results show the specifics of nursing in colorectal surgeries. We found out that nurses working in colorectal surgeries deal mainly with preparing special medical instruments and devices needed for endoscopic examination. Then they assist doctors during a medical examination and with treatment methods of RBL (Barron´s rubber band ligation) and Hemoron, they manipulate with devices in a surgery, check hygiene measures, do administrative work and keep surgery office running. In other conversation areas practical nurses mostly mentioned sampling of biological materials, mainly blood and faecal specimen taking to be given up to histological examination, preparing patients for an endoscopic exam, communication with patients and attending to the intimacy of patients. Consequently we found out that more than half of the practical nurses pay attention to psychological conditioning of the patient before rectoscopy. The research also revealed some weak spots. More than half of the nurses in the focus group do not inform patients about preparations before anoscope exam. Barely one third of the group said that they inform patients about the natural emptying before the exam. Regarding the issue of giving information about lifestyle, we came to the conclusion that practical nurses, even though they do not have enough time, try to inform patients about physical activities, hygiene care and quality of eating and they do it in sufficient rate. But because of the time constrain they put emphasis only on the essential information.
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Comparison of cytokine production by leukocytes from newborns of healthy and allergic mothers
Dusilová, Adéla ; Hrdý, Jiří (advisor) ; Janatková, Ivana (referee)
The increasing incidence of children suffering from allergic diseases could be caused by sensitization of immature immune system during the intrauterine development. Several important scientific papers have demonstrated the ability of cord blood cells to respond by elevated proliferation activity after stimulation by common allergens. Following these findings, present study follows the production of cytokines which play a role in the pro- and anti-allergenic tuning of the immune system. Umbilical cord blood cells were stimulated with polyclonal activators (phytohaemagglutinin) and common allergens (ovalbumin, timothy grass, birch, mite). Subsequently, cytokine production was monitored using selected methods that reflect different stages of cell activation - at the level of mRNA by quantitative real time PCR (qRT-PCR), by flow cytometry detection of the presence of intracellular cytokines in different cell subpopulations and by ELISA measurement of cytokines in CBMC culture supernatants. The results obtained point to a very weak ability of these common allergens (timothy grass, birch, mite, ovalbumin) to stimulate CBMC to produce cytokines observed by all of these methodological procedures. Although we did not observe significant differences in CBMC cytokine production (IL-2, IL-4, IL-10, IL-12,...
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Cultural support in Prague's districts
Mokrá, Jolana ; KAŠPAR, David (advisor) ; PROKOP, Petr (referee)
Diploma thesis ”Cultural support in Prague's districts” focuses on a current situation in municipal strategical management and support of culture in all Prague districts. The introduction emphasises the importance of each level of government in the management and support of culture through comparing specific cultural policies. The research is divided into two parts. First one depicts how culture is being understood on the municipal level and presents an overview of existing instruments for support and planning. The second part is focused on a few municipal districts that handle their culture policy with a particular strategy and consider it to be an irreplaceable part of a local development. The second part also examines what tactics and tools these municipal districts use for a realization of their strategic goals. In conclusion, this thesis proves why the role of the local official government is so important in the developement and implementation of culture.
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Role of Psb28 proteins in the biogenesis of the Photosystem II complex in the cyanobacterium Synechocystis sp. PCC 6803
BEČKOVÁ, Martina
The thesis focuses on the role of Psb28 proteins, namely the Psb28-1 and its homolog Psb28-2, in the biogenesis of the Photosystem II complex (PSII) in the cyanobacterium Synechocystis PCC 6803. The aims of this work were to localize the proteins within the cells, and to determine their function. A fraction of both Psb28 proteins was identified in the monomeric PSII core complexes but most proteins were found in the unassembled protein fraction associated with thylakoid membranes. Psb28-1 was mostly detected as a dimer while Psb28-2 as a monomer. Psb28-1 also differed from Psb28-2 by its higher affinity to the PSII core complex lacking CP43 antenna. Characterization of Psb28-less mutants suggested regulatory function of the proteins in PSII biogenesis in connection with chlorophyll biosynthetic pathway. Analysis of preparations isolated using FLAG-tagged versions of Psb28 proteins showed their association with Photosystem II - Photosystem I supercomplexes, especially under increased irradiance, and supported a role of Photosystem I in the PSII biogenesis.
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Nursing care for long-term bedridden patients with urinary incontinence.
KUBÍKOVÁ, Nikola
Bachelor thesis titled ,,Nursing care for long-term bedridden patients with urinary incontinence" deals with the nursing care of clients with urinary incontinence has long restrained on a bed on the aftercare department. Urinary incontinence is a condition of involuntary leakage of urine, which is a major health and social problem (Kalvach and team, 2004). According to the International Continence Society (ICS) urinary incontinence is classified into extraurethal and urethral. Extraurethal incontinence can be divided into innate and fistulas. The congenital disorder contains atrophy and ectopic urethra. We can divide fistulas into uretral, vesicular and ureteral fistulas. Urethral incontinence are further divided into stress, reflex, urgent and paradoxical ischurii (Locksmith and Macek, 2012). From a nursing perspective is urinary inkontinence divided into nursing diagnoses : Functional incontinence 00020, then Urinary incontinence with overflow 00176, Reflex incontinence 00018, Stress urinary inkontinence 00017, Urge urinary incontinence 00019, Impaired excretion of urine 00016, Urinary retention 00023, Risk of urge urinary incontinence 00022, Efforts to improve the excretion of urine 00166 (Herdman, 2013). The theoretical part of the thesis is talking about the definition of the concept of urinary incontinence and its further division. Also is solving the role of nurses in the diagnosis of myocardial infarction, therapy, nursing process for clients with urinary inkontinence and classification of urinary incontinence by nursing diagnoses with nursing interventions. The practical part of my thesis contains the results of qualitative research, which were obtained by interviews, in-depth interview technique, then content analysis of nursing documentation and comparison of two standards of nursing care of selected hospitals of the Sought Bohemia. The practical outcome of the thesis will be finished client's care plan which is dealing patient long-term bedridden with urinary incontinence.
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