|
|
Non-conventional bacterial signaling pathways
Krupička, Jiří ; Branny, Pavel (advisor) ; Beranová, Jana (referee)
Two component systems were traditionally considered as main phosphorylation systems of bacteria involved in cell signalling. Recently, attention focuses increasingly on bacterial eukaryote-like Ser/Thr protein kinases (eSTKs). These protein kinases are structurally similar to their eukaryotic counterparts. Some eSTKs possess additional domains such as extracellular PASTA domains that were discovered in a variety of gram-positive bacteria. It has been proved that these domains can act as sensors for unlinked peptidoglycan fragments. However, majority of environmental signal molecules still remains unknown. eSTKs phosphorylate a broad spectrum of substrates including proteins involved in various cell processes such as virulence, cell wall biosynthesis, cell division, and central and secondary metabolism. Cross talk between eSTKs and two component systems also occurs. In this thesis, the current knowledge about eSTKs and their significant substrates in different bacterial species is discussed.
|
|
|
Biology of three-toed woodpecker
Navrátil, Martin ; Fuchs, Roman (advisor) ; Reif, Jiří (referee)
In my thesis I have tried to find out how does forest management influence presence of the Three-toed woodpecker Picoides tridactylus.This species is considered a "flagship species" of an untouched primeval type of forest and therefore it requires our attention and protection. The thesis contains results of North-American studies focused on the species very similar to Picoides tridactylus. The main chapters are those aimed to provide information about how is the Picoides tridactylus affected by forest fragmentation, stand health condition and finally the impact of food supply on the occurence and behaviour of the species.
|
|
|
Implementation Analysis of Green Roof on Nursery School Brno-Komin
Jílek, Jan
The article analyzes the construction-technological processes in the implementation of the green roof on the kindergarten building in the city district of Brno-Komín. Firstly, the existing roof was inspected, a new roof was designed, and then the roof structures were reconstructed and supplemented. Depending on the location of the building, it was necessary to solve a suitable time to place the substrate so that it does not blow into the surroundings in case of strong wind.
|
|
|
Possibilities of using recycled materials for the green roofs constructions
Skalková, Markéta ; Žák, Antonín (referee) ; Žižková, Nikol (advisor)
This bachelor thesis deals with the issue of using recycled building materials in the green roofs. It studies the history, function, benefits and negative impacts, composition, division and structural layers of green roofs. The work describes the production, application and common features of recycled building materials. It determines the basic properties of selected recyclates and evaluates their partial suitability as some of the layers of a green roof.
|
|
|
Use of cold kinetic deposition technology on materials used in electrical engineering
Sámel, Maroš ; Zimáková, Jana (referee) ; Binar, Tomáš (advisor)
The purpose of this diploma thesis is to get a better understanding of cold kinetic deposition (cold spray), principles of functioning of this method followed by an evaluation of advantages and disadvantages of cold spray and its comparison to conventional thermal methods and a simple summary of the practical use of cold spray with respect to different materials. Next there is a summary of the properties and uses of frequently applied metals in electrical engineering, aluminium and copper, description of metal corrosion and an understanding of the diagnostic method of acoustic emission. In the practical part, a sample with copper cold spray coating on aluminium substrate was created. Following, this sample was split for corrosion tests, where the split samples were exposed to a corrosive environment for different times of exposure. The extent of corrosion degradation of the samples was evaluated by acoustic emission and metallographic analysis for corrosion-loaded samples for 100, 200 and 300 hours. In the end, an illustrative design of the application of the cold spray technology was created.
|
|
|
Inhibitors of mouse serine racemase
Vorlová, Barbora
Serine racemase (SR) is a pyridoxal-5'-phosphate-dependent enzyme responsible for biosynthesis of D-serine, a recognized neurotransmitter acting as a co-activator of N-methyl- D-aspartate (NMDA) type of glutamate receptors in the mammalian central nervous system. The hyperfunction of the mentioned receptors have been shown to be implicated in many neuropathological conditions including Alzheimer's disease, amyotrophic lateral sclerosis and epilepsy. To alleviate the symptoms of these diseases, several artificial blockers of NMDA receptors have been introduced into the clinical practice. However, many of these compounds cause undesirable side effects and it is thus necessary to search for either less harmful blockers or regulators of other targets of pharmaceutical intervention that are involved in NMDA receptor activation. In this context, specific inhibition of serine racemase seems to be a promising strategy for regulation of NMDA receptor overstimulation. Mouse serine racemase shares 89% identity with its human ortholog and it was also shown that both enzymes possess similar kinetic parameters and inhibitor specificity. Therefore, the mouse models can be used to search for a potent human serine racemase inhibitor. Although many different compounds for their inhibitory potency towards serine...
|
|
|
Production of Multilayer PCB
Janda, Ondřej ; Zatloukal, Miroslav (referee) ; Starý, Jiří (advisor)
This master thesis introduces the problematics of multilayer printed circuit boards. Theoretical part is focused on materials used for production of multilayer PCB and their parameters. At the same time, it focuses on the pressing process of multi-layered PCB, the types of presses used and their advantages and disadvantages. Practical part describes the methodology and testing of the PCB to identify the reliability of chosen electrical parameters. Testing is aimed to simulate thermal stress during machine soldering and during thermal stress in the target environment.
|
|
|
Hot plate with power regulation
Lacika, Marek ; Skácel, Josef (referee) ; Búran, Martin (advisor)
This thesis deals with issues of used hot plates and thick-film technology. The theoretical part of diploma thesis contains a theoretical analysis and description of corresponding technology and usage of materials. The practical part of the thesis focuses on the design of the resulting device, which forms design of the test and optimized hot element, the initial design of the device and the design of models for temperature simulations. Then follows description of the practical realization of the motifs and testing of the created thick-film structure on a ceramic substrate. In the last part are shown simulations of heat transfer in the proposed model of the device.
|
|
|
Estimation of biogas production in biogas plant
Heger, Jan ; Vondra, Marek (referee) ; Touš, Michal (advisor)
The focus of this thesis are calculation methods for biogas yield estimation in biogas plant. The aim is to get acquainted with calculation methods and to determine their accuracy by calculation. Refining biogas production estimates can be useful for case studies, feasibility studies, or technical-economic assessments. The available calculation methods are tested on a case study where a comparison with the operational data measured in the biogas plant in Valašské Meziříčí is accomplished using the extended Buswell method and tabulated values. Comparing this case study, a result was obtained where the calculation by the Buswell method reached very accurate values, whereas the calculation by tabulated values differed more from the measured values and the method achieved a less accurate result.
|
|
|
Inhibitors of mouse serine racemase
Vorlová, Barbora
Serine racemase (SR) is a pyridoxal-5'-phosphate-dependent enzyme responsible for biosynthesis of D-serine, a recognized neurotransmitter acting as a co-activator of N-methyl- D-aspartate (NMDA) type of glutamate receptors in the mammalian central nervous system. The hyperfunction of the mentioned receptors have been shown to be implicated in many neuropathological conditions including Alzheimer's disease, amyotrophic lateral sclerosis and epilepsy. To alleviate the symptoms of these diseases, several artificial blockers of NMDA receptors have been introduced into the clinical practice. However, many of these compounds cause undesirable side effects and it is thus necessary to search for either less harmful blockers or regulators of other targets of pharmaceutical intervention that are involved in NMDA receptor activation. In this context, specific inhibition of serine racemase seems to be a promising strategy for regulation of NMDA receptor overstimulation. Mouse serine racemase shares 89% identity with its human ortholog and it was also shown that both enzymes possess similar kinetic parameters and inhibitor specificity. Therefore, the mouse models can be used to search for a potent human serine racemase inhibitor. Although many different compounds for their inhibitory potency towards serine...
|
guest ::
