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The Usage of Separation Methods for Research of Biologically Active Substances in Waters
Vydrová, Lucie ; Friedl, Zdeněk (referee) ; Opatřilová, Radka (referee) ; Chýlková,, Jaromíra (referee) ; Vávrová, Milada (advisor)
Pharmaceuticals are biological active compounds with different functional groups, physico-chemical and biological properties. These chemical compounds are called as “new contaminants” which cumulate in various environmental components. These contaminants input to environment from industrial processes, hospitals and health care institutions or household sources and these pollutants can to negatively interact with environmental components. Pharmaceuticals are separated according to structure and their effects to live organisms. On the basis of drug consumption in Czech Republic the non-steroidal anti-inflammatory drugs are the most using pharmaceuticals, because these drugs can be obtain without prescriptions. The choosing analgesics for study were monitored in waste water from waste waters treatment plant (WWTP Brno – Modřice) and in surface water from two sampling sites of river Kretinka. Pharmaceuticals were monitored in surface waters in Czech Republic, in Scotland (river Thurso) and in Taiwan (river Erren, Agongdian, Yanshuei and canal of Tainan) Solid phase extraction (SPE) is used for extraction of study pharmaceutical from water system. The high performance liquid chromatography (HPLC) with diode array detection or mass spectrometry was used for determination of drugs in surface waters from Czech Republic. The water samples from Scotland and Taiwan were analysed and the high performance liquid chromatography with mass spectrometry (HPLC/MS) was used for determination of pharmaceuticals. The method of HPLC/MS enables the determination of study compounds in the very low range of concentrations (ng ? l-1). All monitored pharmaceuticals were identified and quantified in water samples from river Kretinka in Czech Republic, river Thurso (Scotland) and rivers Erren, Agongdian, Yanshuei and canal of Tainan in Taiwan and the obtained data were compared.
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Organohalogenic compounds - contaminants of environment
Kociánová, Veronika ; Čáslavský, Josef (referee) ; Vávrová, Milada (advisor)
Polybrominated diphenyl ethers belong to group of brominated flame retardants. Important property of these substances is their ability to reduce flammability or to prevent ignition of many products of daily use, such us televisions, carpets, mattresses and many others. Polybrominated diphenyl ethers however are characterized by high persistence and lipophility, leading to their accumulation in the environment. In recent years, concerns about the effects of organic halogen compounds on human health, suggesting their inclusion in the list of persistent organic pollutants in 2009. These contaminants are capable of long-range transport and accumulation in the environment. Therefore, the presence of polybrominated diphenyl ethers is systematically observed in the abiotic and biotic components of the environment worldwide. Their presence has been demonstrated in areas that are clearly very distant from sources of contamination. This thesis is focused on the use of gas chromatography to assess the level of contamination of selected components of the environment, especially of soil. For these purposes was used method of gas chromatography with electron capture detection mass spectrometry.
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Non-target screening of organic pollutants in waters and sediments
Hamalčíková, Veronika ; Vávrová, Milada (referee) ; Čáslavský, Josef (advisor)
This master thesis deals with the potential influence of the waste dump Hrádek u Pacova and the wastewater treatment plant Brno – Modřice on surroundings using the optimized non-target screening method of organic pollutants in water and sediments. The experimental part is focused on the identification of organic compounds in samples of ground water from monitoring wells, the surface water from stream and pond and in sediment samples collected from streams in the vicinity of municipal waste dump. Organic compounds were also identified in water and sediment samples from the river Svratka near the municipal waste water treatment plant Modřice. The sequential liquid – liquid extraction was used for the isolation of organic compounds in water samples. Organic compounds in sediment samples were isolated by pressurized solvent extraction (PSE) with subsequent fractionation of the extract using column chromatography. Final analysis determination was performed using comprehensive orthogonal two-dimensional gas chromatography with mass spectrometric detection (GCxGC-TOF MS).
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Determination of polybrominated diphenylethers in matrices from fireplaces
Čechová, Eliška ; Hroch, Martin (referee) ; Vávrová, Milada (advisor)
Polybrominated diphenyl ethers belong among the persistent compounds, which have been classified as priority organic pollutants. In environmental compartments are observed in the past decade. Polybrominated diphenyl ethers are detected in abiotic and biotic matrices. For this dissertation were chosen the matrixes from seats of fire, taken in various localities of the Czech Republic and Slovakia. Their analysis should demonstrate whether they in these specific matrices remain. There were examined following polybrominated diphenyl ether congeners: BDE- 28, 47, 99, 100, 153, 154, 183. For their isolation from the matrix were used three different extraction techniques, namely ultrasonic extraction, microwave extraction and pressurized solvent extraction. For the determination was chosen method of GC / ECD. In this dissertation are also described basic chemical, physical and environmental properties of BDE, including other analytical methods that can be used to determine polybrominated diphenyl ethers in environmental compartments.
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Separation of rigid particles from heterogeneous suspension
Prax, Ondřej ; Debreczeni, Ondřej (referee) ; Pochylý, František (advisor)
The bachelor thesis contains a literature search dealing with the separation of solid particles from a heterogenic suspension. The first part is aimed at separation methods. Then, a chapter follows dealing with separation devices, which are used in waste water treatment plants. A more detailed description of the separator used for the separation of sand from waste waters is part of this paper. The described device serving to separate solid particles from a suspension uses the combination of sedimentation and separation methods. To visualize the separator a 3D model in Autodesk Inventor Professional 9 device was used in the presented paper.
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Influence of the municipal waste dump on the environment
Hamalčíková, Veronika ; Vávrová, Milada (referee) ; Čáslavský, Josef (advisor)
This bachelor thesis deals with the potential influence of the waste dump on the environment, particularly on the water quality close to waste dump. The experimental part is focused on the identification of organic compounds in samples of water from monitoring wells and in samples of surface water from stream and pond. Solid phase microextraction (SPME) was employed for the isolation of organic compounds and gas chromatography with mass spectrometric detection (GC/MS) was used for their analysis. Obtained results were evaluated and their interpretation was done.
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Determination of selected drugs by separation methods
Skálová, Lucie ; Čáslavský, Josef (referee) ; Vávrová, Milada (advisor)
This work summarizes the basic information regarding the occurence and incidence of pharmaceutical residues in the environment, causes of their existence in the environmental components, their effects and consequences arising. In addition, provides information related to the problem of pharmaceutical waste and its disposal. Part of the work is an overview of specific drugs from the group of analgesics, and a description of the various steps and methods suitable for their determination in aqueous matrices.
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Residuals of pharmaceuticals in waters
Vašíčková, Petra ; Čáslavský, Josef (referee) ; Vávrová, Milada (advisor)
This bachelor thesis focuses on studying synthetic pharmaceuticals spreading into the aquatic ecosystem. Ketoprofen and diclofenac, non-steroidal analgetics were studied. The aim of this work is to develop both a suitable analytic method for analytes isolation from a given matrix and an optimal technique for identification and determination of analgetics residues in waters. Solid phase extraction (SPE) was employed as the separation method for isolation of given pharmaceuticals and high performance liquid chromatography (HPLC) with diode-array detection was used for their determination. The optimised and validation method was used to model samples of water. Obtained results were evaluated and their interpretation was done.
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