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Immunotherapy of HPV16 - associated cancers and regulation of antitumour immune response
Štěpánek, Ivan ; Reiniš, Milan (advisor) ; Lipoldová, Marie (referee) ; Němečková, Šárka (referee)
The MHC class I status of tumour cells during immunotherapy is often underestimated. It represents one of important tumour escape mechanisms and thus can contribute to the failure of most of the cancer clinical trials that are usually based on the induction of cytotoxic T cell responses. Epigenetic changes in the promoters of genes involved in the MHC class I Ag presentation can result in decreased expression of the cell surface MHC molecules on tumour cells. Thus, epigenetic modifiers can restore an expression of the MHC class I molecules and make tumours visible to the CD8+ effector cells. Besides the epigenetic changes on the tumour cells, epigenetic modulators affect cells of the immune system such as dendritic cells (DC). Tumour cells can escape from the immune response not only by changes in the cancer cells, but also by influencing, expanding and/or activating immunoregulatory cell populations, such as regulatory T cells (Treg). This thesis focuses on the potential of the DC-based vaccines against HPV-16-associated tumours with a different MHC class I expression, on the combination of cancer immunotherapy with the treatment using epigenetic modifiers, with special attention paid to their effects on DC, and, finally, on the impacts of the anti-CD25 antibody (used for Treg elimination) on Treg and NKT...
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Assessment of Vaccination Coverage Against Influenza among Students of the University of South Bohemia in the České Budějovice
RUDKOVÁ, Eva
This thesis deals with the evaluation of vaccination against influenza among students of University of South Bohemia, their opinions and their approach to the issue of vaccination against influenza. The theoretical part is focused on historical, clinical, diagnostic, virological, immunological and epidemiological aspects of influenza vaccine development and vaccination against influenza. The aim of the practical part was to determine how widespread is influenza vaccination among students of University of South Bohemia in Ceske Budejovice, map their opinion on the vaccination and to assess their awareness regarding vaccination against influenza and influenza in general. For research purposes a questionnaire survey was distributed among students of the University of South Bohemia. The selection of respondents was conducted by stratified random selection so that it covered approximately 5% occupancy of each faculty. This research preceded a preliminary research to determine the suitability of the chosen method of data collection. As a data collection instrument was chosen the method of questioning by the form of questionnaires. Quota for the research was to study the respondents at the South Bohemian University and studied faculty. In total 468 questionnaires were evaluated. The results of the research shows that students of University of South Bohemia underestimate vaccination, and most of them have never been vaccinated against this disease. In spite of this is, the results show that the awareness of students is good and among faculties except the Faculty of Health and Social Care, there is not a significant difference. Faculty of Health and Social achieved in the overall awareness significantly higher result than other faculty. Thus confirming the hypothesis that there are differences between students' awareness of the Health and Social faculty and students of other faculties. The aim of this thesis is to alert students of the University of South Bohemia about the pros of influenza vaccination and increase their awareness in this area.
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