|
|
Non-conventional bacterial signaling pathways
Krupička, Jiří ; Branny, Pavel (advisor) ; Beranová, Jana (referee)
Two component systems were traditionally considered as main phosphorylation systems of bacteria involved in cell signalling. Recently, attention focuses increasingly on bacterial eukaryote-like Ser/Thr protein kinases (eSTKs). These protein kinases are structurally similar to their eukaryotic counterparts. Some eSTKs possess additional domains such as extracellular PASTA domains that were discovered in a variety of gram-positive bacteria. It has been proved that these domains can act as sensors for unlinked peptidoglycan fragments. However, majority of environmental signal molecules still remains unknown. eSTKs phosphorylate a broad spectrum of substrates including proteins involved in various cell processes such as virulence, cell wall biosynthesis, cell division, and central and secondary metabolism. Cross talk between eSTKs and two component systems also occurs. In this thesis, the current knowledge about eSTKs and their significant substrates in different bacterial species is discussed.
|
|
|
Rezistence sóji luštinaté k původci bakteriální spály Pseudomonas syringae pv. glycinea
Coufalová, Tereza
This thesis describes fundamental biological characteristics of soybean. Main topic of the thesis is finding resistence of twelve soybean varieties to bacterial blight. Virulence of four Pseudomonas syringae pv. glycinea isolates was tested in laboratory conditions to find isolates with the highest virulence, which were then used for testing of selected soybean varieties resistance to this patogen. The highest virulence was found in Šumperk 112 isolate and Žabčice isolate. These isolates were used for testing of soybean varieties resistance. The lowest resistance was found in Moravians variety, low resistance was found in Laurentiana variety and Bru-nensis variety. High resistance was found in Gallec variety and Chinese NM3 variety.
|
|
|
Nucleotide variability in \kur{enol-1} gene of entomopathogenic nematodes \kur{Heterorhabditis bacteriophora} and \kur{Steinernema feltiae}
HEJNÍČKOVÁ, Martina
The topic of this bachelor thesis is enol-1, parasitism-related gene in entomopathogenic nematodes. First part of this thesis deals with pattern of the enol-1 nucleotide variability. Nucleotide sequence of enol-1 was obtained from 9 strains of Heterorhabditis bacteriophora and 8 strains of Steinernema feltiae. I characterised and compared its structure and nucleotide variability. Phylogenetic analysis of enol-1 was done with closely related nematode coding sequences, acquired from internet databases. The second part of this thesis is devoted to virulence testing. Larvae of Galleria mellonella were infected with different strains of entomopathogenic nematodes, and their mortality was summarized.
|
|
|
Risks of salmonellosis and prvention of this disease in public health education
VAČKÁŘ, David
This bachelor thesis deals with the origin and development of salmonellosis and the risks related to the bacterial disease. Symptoms of the disease as well as the varied ways the infection spreads are described. Risks associated to storing, processing, and taking in food are pointed out. These are related to prevention of the disease and its treatment when it develops, focused on fluid and food intake, too. The thesis includes a questionnaire survey about this diarrheal disease, with a concluding processing and evaluation of the questionnaires.
|
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
guest ::
