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Physiotherapy using rhythmic auditory stimulation in upper limb training for patients with motor disorder caused by brain damage
Volchanskaya, Sofya ; Táborská, Silvie (advisor) ; Sládková, Petra (referee)
Title: Physiotherapy using rhythmic auditory stimulation in upper limb training for patients with motor disorder caused by brain damage Abstract: The theme of this bachelor thesis is physiotherapy using rhythmic auditory stimulation in upper limb training for patients with motor disorder caused by brain damage. The thesis consists from theoretical and practical parts. The range of problems associated with brain damage, both traumatic and stroke-induced, is briefly described in theoretical part. Fine and gross motor function of upper limb, the impact of central nervous system damage on it and possibilities of subsequent physiotherapy are also described in this part. In the last chapter of theoretical part knowledge about musical therapy, its application in neurorehabilitation and Rhythmical Auditory Stimulation method (RAS) is summarized. Practical part consists of two case reports of patients with acquired brain damage and following upper limb moving disorder. In this part the influence of RAS method during therapy is monitored. The evaluate the method's effect Five-Step Clinical Assessment by Gracies and Jebson-Taylor standard hand function test were used prior to initiation of treatment and immediately after its completion. The purpose of this thesis is summarizing and comparing knowledge about RAS method...
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The influence of rhythmic auditory stimulation on gait-pattern of patients with brain damage brain damage
Pavlikhina, Ekaterina ; Táborská, Silvie (advisor) ; Konopáčová, Eva (referee)
Author: Ekaterina Pavlikhina Supervisor: Mgr. Silvie Táborská Title of bachelors thesis: The influence of rhythmic auditory stimulation on gait-pattern of patients with brain damage Abstract: The Bachelor's thesis is focused on influences of musical therapy method RAS (Rhythmic Auditory Stimulation) upon the quality of patient's walking with the brain injury. The thesis summarizes and compares theoretical knowledges of music therapy in patients with pathological gait-pattern due to brain damage. Theoretical part of the Bachelor's thesis is intended to focus on the brain injury both caused by a trauma and an infarct. In the following part are described the physiologic and pathological mechanisms of walking. The final chapter of the theoretical part introduces the exact principles of musical therapy, it's variants and technics that can be used in the treatment of patients. In this chapter I have also referred on the studies that correlate the topic. The practical part contains two casuistries of the patients with the brain injury, where is described kinesiology and the therapeutic exercises in the way of the program. In order to esteem the progress of applicated therapy I used the special "walking tests". Key words: brain damage, music therapy, pathological gait-pattern, Rhythmic Auditory Stimulation, RAS
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Structure- and sequence-based identification of functionally important amino acids in a protein family
Peclinovská, Iveta ; Novotný, Marian (advisor) ; Pleskot, Roman (referee)
A group of small GTPases consist of over twenty protein families in the super class P-loop. It has a very diverse cell functions. Small GTPases regulate the formation of vesicular follicles, cytoskeleton and nuclear transport. They participate also on cell proliferation and signaling. The aim of my work is to find important amino acids that define family and distinguish each other. I focus on families Arf, Rab, Ran, Ras and Rho. At the Rho family I am also devoted to classes Rho, Rac and Cdc42. Amino acids are identified using bioinformatic programs selected Consurf and Sca5. The objective is also to test P2RANK specialized tool developed at the Charles University in Prague that predict ligand binding sites from protein structure in different families. Founding amino acids can have a big role in the functional divergence of individual families and classes of small GTPases and can be the basis for future study example for the proliferation of cancerous cells. 1.1 Keywords Powered by TCPDF (www.tcpdf.org)
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The comparison of properties of cell lines resistant to ellipticine, doxorubicin, and cisplatin
Černá, Tereza ; Poljaková, Jitka (advisor) ; Eckschlager, Tomáš (referee)
7 Abstract Neuroblastoma is the most common extracranial solid tumor of childhood. Despite advances in cancer diagnosis and therapy, the treatment of some forms of neuroblastoma is still complicated. One of the major complications of the chemotherapy is a developed drug resistance. This master thesis deals with the effect of cytostatics on protein and gene expression of selected proteins, which may contribute to chemoresistance of the human neuroblastoma cell line UKF-NB-4. The sensitive line UKF-NB-4 and the resistant line UKF-NB-4CDDP , UKF-NB-4DOXO and UKF-NB-4ELLI were exposed to cisplatin, doxorubicin, ellipticine for 24, 48 and 72 hours. The Western blot analysis showed that cytostatic agents cisplatin, doxorubicin or ellipticine added to the sensitive neuroblastoma cell line UKF-NB-4 in amounts which are added to resistant neuroblastoma cell lines in order to maintain resistance induced expression of p53 and reduced expression of retinoblastoma protein pRb after 72 hours of cultivation. Differences in the expression of RAS protein, cytochrome P450 1A1, 3A4 and cytochrome b5 has not been shown. Changes in the expression of the studied proteins in resistant lines UKF-NB-4CDDP , UKF-NB-4DOXO and UKF-NB-4ELLI cultured with and without cytostatic agents were not detected by the Western blot analysis....
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The function of palmitoylation of membrane proteins in immune cells
Hanusová, Zdeňka ; Otáhal, Pavel (advisor) ; Pavlů, Barbora (referee)
Protein palmitoylation is a post-translation modification, which typically regulates the protein interaction with a membrane; apart from that, it can have various functions in protein regulation. Process of this modification covers covalent attachment of palmitate to an aminoacid residue in the target protein; identity of the aminoacid than determines the palmitoylation type. Most common is modification of a cystein residue - in that case we speak about so-called S-palmitoiyation. The exceptionality of this lipid modification type is in the reversibility of the whole process; this enables for example trafficking regulation of many proteins between various membrane compartments and ragulation of proteins' function. In vivo, palmitoylation of many proteins is mediated by enzymes protein acyltransferases, while depalmitoylation is mediated by enzymes acylprotein thioesterases. Palmitoylated proteins can be identified in wide spectrum of cellular types, also in immune cells. Palmitoylation plays here an important role, especially in interaction of signal proteins with lipid rafts and the related modulation of protein's function. Significance has palmitoylation also as a dynamic process, which mediates the right identification of protein's subcellular localization. This work is a review and it's aim is...
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Project of Client-based VPN Remote Access Solution for the worldwide Retail Business Company in the region of Central Europe
Ondrák, Richard ; Havlín, Petr (referee) ; Kříž, Jiří (advisor)
The aim of this Thesis is to project and describe new Client-based Remote Access Solution for the Tesco Stores retail company in the region of Central Europe. Based on facts that the all present Tesco’s Remote Access Systems have not any longer met the increasing requirements and needs of corporate users and suppliers it is clear that the main scope is to find and implement such Solution that will meet and exceed all requirements. The main Project Aspects are to be the ease of use, security of the solution, unexacting administration of the system and user groups, and one unified Solution for the all Countries from the region of Central Europe that is suitably integrated in the Tesco IT infrastructure.
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Length and weight growth of early pikeperch fry fed with enriched artemia nauplii in experimental conditions
JANKOVÝCH, Antonín
The aim of our experiment was proving the suitability and effectivity of artemia nauplii enrichment in pikeperch fry rearing. 24 hour enrichment took place in 3 variants: 1. SELCO, 2. 18 (n-3) (alfa-linolenic acid) and 3. 18 (n-3) + vitamin C. Original intention was initializing fry feeding with different types of enriched artemia metanauplii. Due to excessive size of artemia after 1 day enrichment (Artemia franciscana) (in comparison with size of fry oral cavity) the intake of such food was not possible, thus the metodics had to be modified. From the given reason, fish were initially fed with unenriched nauplii ; firstly with dose 100 ml (4 - 8 day post hatch - dph) after that with dose 50 ml per tank (9. dph 25.4.). As soon as fry reached suitable size and was able to intake bigger size of nauplii, was in the second part feeding diferenciated (use of different types of enrichment and control group was fed with unenriched artemia). First part of experiment lasted 22 days (5.4. - 26.4.), fish must have been netted and again placed in tanks for experiment with enriched artemia. Second part of rearing varied in use of differently enriched metanauplii and lasted 8 days (27.4. - 4.5.). At the end of second part of feeding (from 1.5.) was involved co-feeding, which means combined feeding (living food and starter feed). 5.5. was experiment terminated. The highest survival was reached in group fed with artemia enriched in comercial preparate SELCO (28,67 %), second highest survival was reached in group fed metanauplii enriched in alfa-linolenic acid (27,88 %) and third highest survival was reached in group fed with alfa-linolenic acid and vitamin C enriched metanauplii (19,00 %). The highest individual fry weight at the end of experiment was reached in group fed with artemia enriched in alfa-linolenic acid and vitamin C : 306,16 +- 64,27 mg, the lowest individual weight was reached in control group : 216,9 +- 39,96 mg. The highest average total lenght (l.t.) was measured in SELCO group 30,13 +- 2,47 mm, the lowest total lenght reached fish in control group 27,37 +- 1,32 mm. In group fed with artemia enriched in vitamin C was noticed the highest percentage of starter feed intake (8 %), but simultaneously in the same group was reached the highest rate of cannibalism (6 %).
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Influence of Light Regime on the Success of Adaptation of Pike (\kur{Esox lucius}) Larvae on Pelleted Feed in RAS
DUŠEK, Tomáš
The objective of this thesis for a bachelor´s degree was to experimentally evaluate possibilities of adaptation of northern pike (Esox lucius) for controlled breeding conditions in the RAS and for intake of artificial pelleted feed. Special attention was focused on the influence of light regime on the success of adaptation and the rate of cannibalism. The experiment was divided into six groups with different light regimes (L8 : D16, L4 : D8 : L4 : D8, L12 : D12, L4 : D4 : L4 : D4 : L4 : D4, L16 : D8, L8 : D4 : L8 : D4). Into each group were putted 3,600 pieces of pike larvae, which means 20 pcs/l. Feeding was carried out manually in 15 minutes time interval. Daily mortality was recorded and biometric measurements were conducted. After 15 days, the experiment was evaluated. Adaptation for intake of pelleted feed was successful. As was found out, the light regime has the influence on survival, cannibalism and growth of northern pike in RAS. The highest final piece weight was achieved under the light regime L16 : D8 (W= 0,13135 ? 0,02924 g). The lowest final piece weight was achieved under the light regime L8 : D16 (W= 0,06296 ? 0,01306 g). The highest survival was achieved under the light regime L8 : D4 : L8 : D4 (61,36 %), the worst under the light regime L4 : D8 : L4 : D8 (28,33 %).
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Optimization of harvest of juveniles of pikeperch (\kur{Sander lucioperca}) taken out of ponds and following ability of the fish to adapt to system RAS
HAMPL, Jan
Within my bachelor thesis I have been trying to resolve the possibilities of optimization of harvest of juveniles of pikeperch (Sander lucioperca) taken out of ponds, and continuously I have been tracking the ability of the fish to adapt to system RAS that is applied at the experimental fish culture facilities at FROV JU. Working at the fish harvest it has shown me to be important to synchronize the work of every one of the assistants during the individual activities. The fish have been harvested in a very short time and carefully taken to already prepared transport boxes. We checked the most important parameters for the quality of water, its temperature and oxygen saturation. The mortality of the fish during the harvest and transport did not get over 2 %. Just after the transport to experimental fish culture facilities at FROV JU, to the system RAS at Vodnany, the harvested fish has been placed to 9 tanks for the weaning period. Each out of three ponds always received three tanks of harvested and adapted fish. The experiment at RAS has been divided into three parts. First part has been about the weaning period of the fish to the RAS system and the artificial food. The results were very satisfying. I have been following these parameters: SGR, FCR, increase of biomass, survival and cannibalism at the tanks. The level of survival from the pond Rohlíček has been around 83,6 %, the pond Bejkovna 69,7 % and Horažďovice 4 the level of survival was 71,8 %. After finalizing the weaning period we did biometry for 37 of selected representatives. Second part has been the 1st post-weaning period. Here I focused at the parameters such as SGR, FCR, increase of biomass, survival and cannibalism at the tanks. The level of survival from the pond Rohlíček has been around 84,17 %, the pond Bejkovna 83,83 % and Horažďovice 4 the level of survival was 82,83 %. After finalizing the 1st post-weaning period we did biometry for 37 of selected representatives. Third part has been the 2nd post-weaning period. Here I focused at the same parameters as above such as SGR, FCR, increase of biomass, survival and cannibalism at the tanks. The level of survival from the pond Rohlíček has been around 87,5 %, the pond Bejkovna 88,17 % and Horažďovice 4 the level of survival was 87,17 %. After finalizing the 2nd post-weaning period we did biometry for 37 of selected representatives, the experiment was evaluated and pikeperch juveniles were sold to the French company Asialor.
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Adaptation of northern pike (Esox lucius L.) on artificial pelleted feed under controlled conditions
HAJÍČEK, Jiří
The main objective of this thesis is to describe and experimentally evaluate the possibility of adaptation of pike (Esox lucius L.) in controlled housing conditions and income artificial pelleted feed. The thesis is mainly based on the effect of an initial larval density of pike for adaptation and survival and the growth of larvae reared in the rearing tanks. The experiment consisted of four different densities of larvae (10, 20, 40, 80 pc of the larvae l??, which were always repeated three times. Two experiments were performed (M, H), with a length of rearing of five days. Rearing itself was conducted in twelve circular tanks with a capacity of 180 l??, the recirculation system connected to the experimental rearing facility in Vodňany FROV JU. The temperature during rearing was constant. For the first experiment the temperature was 22.3 ? 03 °C. For the second one, the average temperature was 21.5 ? 0.6 °C. The frequency of feeding was continuous (24 h) with a combination of hand feeding and feeders with a timer (at night). The illumination of the aquarium tanks was provided by fluorescent lamps, for each tank individually. The light intensity was high (210 lux) and constant during the rearing. Both experiments showed the optimal density at the initial feeding from 20 to 40 units . l??. Also, we have implemented made out performed- the basic biometric analysis of W (g) and TL (mm), the body length, size of eyes, head, jaw length, prenatal length.
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