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Support for Nurses in Death Cases at Resuscitation Units and Intensive Care Units
LAYEROVÁ, Helena
At Resuscitation Units and Intensive Care Units nurses face the issue of dying and death every day. Very often they are in contact with the dying and their immediate family and they are expected to have professional approach and provide comprehensive care. Stress that the nurses experience in these situations results into mental, emotional and physical exhaustion, and can be manifested by psychosomatic problems. Preparation of nurses to these situations and support of their management is therefore a key factor for the management of this demanding profession. The theoretical part of the thesis contains a description of the current state of the issue, characterizes resuscitation and intensive care, and deals with the issue of death and dying in Resuscitation Units nad Intensive Care Units, managing situations associated with death and the support of nurses in these situations. The work has four goals. The first objective is to determine what method of preparation of nurses is in practice ensured in Resuscitation Units and Intensive Care Units for the performance of high-quality nursing care for dying patients. Another objective is to determine whether and how the nurses are prepared and trained in the field of management of situations connected with the death of a patient in Resuscitation Units and Intensive Care Units. The third objective is, whether there is and what is the form of assistance to nurses in RU and ICU provided by the team and the management of the Department in case of deaths of patients, and the fourth objective is to determine what form and manner of support nurses expect and require. Research part of the thesis was carried out by quantitative investigation using an anonymous questionnaire. Respondents were answering 38 questions, 13 questions were closed, 21 half open and 4 were open. The support is most frequently understood as a psychological support, good teamwork, communication, supervision and representation where necessary. Management is expected primarily to provide support through education, ensuring supervision, corresponding financial support and professional psychological assistance where necessary. On the basis of this work?s results a proposal for the course for paramedical professions working in RS and ICU called ?Course of the Strategy of Difficult Situations Management in the Care for the Dying and Their Close Family in Intensive and Urgent Care?, was made.

Marketing strategy adaptation of Chipotle in Czech markets
Hofmannová, Veronika ; Král, Petr (advisor) ; Janecký, Vlastimil (referee)
This Diploma thesis deals with chain of fast food restaurants named Chipotle, which is predominantly known in the United States of America, and its potential entry into the Czech market. The thesis analyzes the marketing strategy of the company and applies its adaptation for the Czech market. The competitive advantage of the company is using local organic raw ingredients of high quality; therefore, the thesis examines the Czech people´s interest in Mexican cuisine as well as issues of sustainable development. The aim of the thesis is to analyze the marketing strategy of the chain of restaurants with Mexican fast food and subsequently to apply the adaptation of the marketing mix to the conditions at the Czech market.

The current state and prospects for sustainable development of tourism in Slovakia.
Libantová, Silvia ; Indrová, Jarmila (advisor) ; Chaloupka, Radek (referee)
Tourism contributes to the prosperity of destinations by bringing financial resources, new jobs, a higher level of services and infrastructure development. However, if tourism does not develop sustainably, it is accompanied by negative factors, as well. Especially, negative impacts on the environment, such as: water and soil contamination, increased noise and overcrowded destination during the high season. As a response to the worsening state of the environment and possible irreversible changes caused by irresponsible human behavior, the idea of sustainable development is becoming more important. The theoretical part describes tourism in the Slovak Republic and highlights plans for sustainable tourism development. In the practical part, the results of the questionnaire, related to the awareness of sustainable tourism in the Slovak Republic, are evaluated. The main objective of this thesis is to evaluate the current situation in the field of sustainable tourism and to make suggestions for its future development.

Changes in structure and phase composition in the surface of tram rail
Švábenská, Eva ; Roupcová, Pavla ; Schneeweiss, Oldřich
We have investigated structure and phase composition of surface layer of tram rails after long time running and the results were compared with those obtained on the original part of material. Changes due to effects of severe plastic deformation together with thermal shocks by friction process were expected. The information about structure and phase composition was obtained by optical and scanning electron microscopy, X-Ray Powder Diffraction, Mössbauer Spectroscopy and Glow Discharge Emission Spectroscopy (GDOES) and this was completed by microhardness measurements. The results show that the surface layer in comparison with the original material exhibits important changes in grain structure, an increase in microhardness and high content of iron oxide and hydrooxides. According to the depth profile of the chemical composition measured by GDOES there is an increase in carbon content in the surface layer which can be effect of up-hill diffusion.

A Comparison of Preconditioning Methods for Saddle Point Problems with an Application to Porous Media Flow Problems
Axelsson, Owe ; Blaheta, Radim ; Hasal, Martin
The paper overviews and compares some block preconditioners for the solution of saddle point systems, especially systems arising from the Brinkman model of porous media flow. The considered preconditioners involve different Schur complements as inverse free Schur complement in HSS (Hermitian - Skew Hermitian Splitting preconditioner), Schur complement to the velocity matrix and finally Schur complement to a regularization block in the augmented matrix preconditioner. The inverses appearing in most of the considered Schur complements are approximated by simple sparse approximation techniques as element-by-element and Frobenius norm minimization approaches. A special interest is devoted to problems involving various Darcy, Stokes and Brinkman flow regions, the efficiency of preconditioners in this case is demonstrated by some numerical experiments.

STRAIN ENGINEERING OF THE ELECTRONIC STRUCTURE OF 2D MATERIALS
del Corro, Elena ; Peňa-Alvarez, M. ; Morales-García, A. ; Bouša, Milan ; Řáhová, Jaroslava ; Kavan, Ladislav ; Kalbáč, Martin ; Frank, Otakar
The research on graphene has attracted much attention since its first successful preparation in 2004. It possesses many unique properties, such as an extreme stiffness and strength, high electron mobility, ballistic transport even at room temperature, superior thermal conductivity and many others. The affection for graphene was followed swiftly by a keen interest in other two dimensional materials like transition metal dichalcogenides. As has been predicted and in part proven experimentally, the electronic properties of these materials can be modified by various means. The most common ones include covalent or non-covalent chemistry, electrochemical, gate or atomic doping, or quantum confinement. None of these methods has proven universal enough in terms of the devices' characteristics or scalability. However, another approach is known mechanical strain/stress, but experiments in that direction are scarce, in spite of their high promises.\nThe primary challenge consists in the understanding of the mechanical properties of 2D materials and in the ability to quantify the lattice deformation. Several techniques can be then used to apply strain to the specimens and thus to induce changes in their electronic structure. We will review their basic concepts and some of the examples so far documented experimentally and/or theoretically.

Micro-electromembrane extraction across multiple aqueous and organic phases for selective pretreatment of raw biological samples
Kubáň, Pavel ; Boček, Petr ; Seip, K. F. ; Gjelstad, A. ; Pedersen-Bjergaard, S.
Micro-electromembrane extraction (μ-EME) across multiple aqueous and organic\nsolutions was developed for selective extractions of various analytes from untreated\nbiological samples. The novel approach requires μL volumes of samples and organic\nsolvents, which form consecutive immiscible plugs in a transparent polymeric capillary\nand the extraction process is accelerated by application of d.c. voltage. Number of the\nplugs, their composition, volume and sequence in the extraction capillary can be chosen\nspecifically for each particular application and high variability and selectivity of the\nextraction process can be achieved. In this contribution, we demonstrate suitability of\na five-phase μ-EME system for simultaneous extractions of cations and anions from\nurine and for selective extractions of basic analytes based on their acid-base strength\nfrom plasma.

Highly sensitive analysis of chlorophenols and sulfonamides in waters by electrophoretic focusion on inverse electromigration dispersion gradient with ESI-MS detection
Malá, Zdeňka ; Gebauer, Petr ; Boček, Petr
This work presents a new methodology for high-sensitivity analyses by capillary\nelectrophoresis (CE) with electrospray-ionization mass spectrometric (ESI-MS)\ndetection, based on electrophoretic focusing on an inverse electromigration dispersion\n(EMD) gradient. The technique is based on a robust electrolyte system providing a\ngradient of required properties, suitable for routine analyses of trace amounts of weak\nacids with pKa values between approx. 6.5 and 9. Examples analyses of several\nchlorophenols and sulfonamides are presented, with LODs around 3x10-9 M for spiked\ndrinking water without any sample pretreatment.

Investigation of bioactive proteins in pressurized water extract of Sambucus nigra L. branches
Hohnová, Barbora ; Šalplachta, Jiří ; Roth, Michal
Investigation of high-value substances in plant materials has been of increasing interest\nin last years. In this study, PHWE, SDS-PAGE and MALDI-TOF techniques were\nemployed for investigation of bioactive proteins in Sambucus nigra L. branches.

ELECTRON BEAM REMELTING OF PLASMA SPRAYED ALUMINA COATINGS
Matějíček, Jiří ; Veverka, J. ; Čížek, J. ; Kouřil, J.
Plasma sprayed alumina coatings find numerous applications in various fields, where they enhance the properties of the base material. Examples include thermal barriers, wear resistance, electrical insulation, and diffusion and corrosion barriers. A typical structure of plasma sprayed coatings, containing a multitude of voids and imperfectly bonded interfaces, gives them unique properties - particularly low thermal conductivity, high strain tolerance, etc. However, for certain applications such as permeation barriers or wear resistance, these voids may be detrimental.\nThis paper reports on the first experiments with remelting of plasma sprayed alumina coatings by electron beam technology, with the purpose of densifying the coatings and thereby eliminating the voids. Throughout the study, several parameters of the e-beam device were varied - beam current, traverse velocity and number of passes. The treated coatings were observed by light and electron microscopy and the thickness, structure and surface morphology of the remelted layer were determined and correlated with the process parameters. Based on the first series of experiments, the e-beam settings leading to dense and smooth remelted layer of sufficient thickness were obtained. In this layer, a change of phase composition and a marked increase in hardness were observed.\n