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Characterisation of the cell line TRAMP-C2 side population, mouse model of prostate cancer
Žlabová, Anna ; Reiniš, Milan (advisor) ; Šmahel, Michal (referee)
Side population is a minor subpopulation (SP) of some cell lines, exporting staining dye Hoechst 33342 out of their cytoplasm. It is discussed as a possible source of "cancer stem cells", "tumour initiating cells" or "metastasis initiating cells". However, broad literature suggest, that stemness and other privileged properties of SP are very variable between different cell types, cell lines and stage of disease. Cell lines TRAMP are the only widely available murine models for testing of prostate cancer therapy. We noticed in literature a mention about existence of 1-2% of cells constituting side population, but detailed characteristic have not been described until now. In this diploma thesis, we worked on characterisation of SP of the TRAMP-C2 cell line in comparison to other cells (nonSP). In the first part, we compared stem properties of SP and nonSP. We started with checking the existence of SP by its verapamil sensitivity. Using mRNA analysis, we showed that neither SP nor nonSP have increased c-Kit expression and that there are no differences in Bmi-1 expression. We found that SP is heterogenic mixture of CD24-CD44-, CD24-CD44+ and CD24+CD44+ cells, while nonSP is almost solely CD24-CD44+. We documented that SP and nonSP returned back to original SP ratio during cultivation. Then we showed on...
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The model of adaptive behavior for the development of Czech assessment scales for children
Chadimová, L. ; Urbánek, Tomáš ; Seifert, M. ; Ivanková, K.
We introduce results of cluster analysis conducted during the development of Czech assessment scales of adaptive behavior in children and adolescents. Within the frame of National Institute of Education we organized the group of experts on the topic of adaptive behavior, both from the academical and practical areas. Every member of the expert group obtained 300 items inspired by local or foreign methods of adaptive behavior assessment. The experts were instrued to cathegorize the items, which they found meaningful with regard to the construct of adaptive behavior and its manifestation on the Czech background. Based on results we compiled consequent model of adaptive behavior, that becomes the base structure for newly developed assessment scales. We compare this structure with the model of adaptive behavior used by DSM-V.\n
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Plant biomass of reclaimed and unreclaimed heps of various age
Dvorščík, Petr ; Frouz, Jan (advisor) ; Cudlín, Pavel (referee)
This thesis has been elaborated as a part of complex succession research on the Velká podkrušnohorská spoil heap near Sokolov (north-west Bohemia, GPS: 50ř13'34.695"N, 12ř42'6.627"E) . The objective of this work is to describe biomass development of reclamational and successional localities on a chronosequence example. Unreclaimed areas are represented by successional spontaneous vegetation with dominant Goat willow (Salix caprea) with an mixture of European aspen (Populus tremula) and European birch (Betula pendula). Reclamation areas are represented by forest planting of Black alder (Alnus glutinosa) mixed with Grey alder (Alnus incana). On this localities was quantify total biomass (above and below ground biomass of herb and tree layer). Data evaluation was performed using allometric equations formed on destructive analysis of the main tree types. There is not statistically significant difference betwen the recovery site methods. Keywords: chronosequence, biomass, succession, reclamation, spoil-heap, allometric
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The occurrence of domestic and wild forms of the camels in the old world and their economic use
Burgerová, Michaela ; Gardiánová, Ivana (advisor) ; Jan, Jan (referee)
Genus camel (Camelus) includes two species, the Dromedary camel (Camelus dromedarius) and Bactrian camel (Camelus bactrianus). Camels have a wide range of utilization. People in Arabia countries but also in India and Egypt are using camels as a milk and meat animal, or even as a transport and companion animals and riding animals too. The aim of this thesis was to find sources about camel keeping and using and his role of farm animal in different cultures and countries. And about conditions and requirements in captivity. The first part of thesis contains information about phylogeny and origin of the camel family, taxonomy of camels, short information about the history of domestication and using of camels. There are also described the two species in terms of species biology etc. plus a brief mention of camel hybrids and camel breeds. The second part focuses on the use of camels. The preffered and most common use is in the production of milk, other dairy products from camel milk, fermentation of milk. Another use is for meat performance, meat composition, describes products. His utilization i also useful for other parts such as hair wool and mane, they come mainly from bactrian camel (Camelus bactrianus). The last is working utilization of camels, carrying baggage. Then hobby use. The thesis describes the conditions of keeping camels in captivity, keeping camels in Somalia, requirements for the supply of water and food, housing conditions in the Czech Republic, it is particularly widespread keeping camels in zoos. In Czech Republic they are small private camel breeders. For comparison they are conditions for keeping camels in captivity in Australia.
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Tenancy spouses and use of the flat spouses in a new arrangement after January 1, 2014
Prantlová, Soňa ; Kadlecová, Eva (advisor) ; Pavla, Pavla (referee)
The thesis is devoted to the topic of tenancy by the spouses, and its use as is specified in the new adaptation of the Civil Code no. 89/2012 Coll. It replaced until functioning Civil Code from the year 1964. The new legislation is specified series of new institutes, whose primary aim is to protect the weaker party, in the case of tenant. The diploma thesis was divided into theoretical and practical part. In the theoretical part, was attention paid to basic concepts which have been defined. There were characterized rights of tenant and landlord, analyzed housing legislation according to the new Civil Code. The practical part was devoted to the interpretation of survey results. There were approached tenants of several apartment buildings in Kralupy nad Vltavou. Based on the findings, were designed some recommendations to raise awareness about the rights and responsibilities of tenants, as well as about the whole issue of housing legally.
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Bank fees in Czech Republic
Burešová, Adéla ; Steininger, Michal (advisor) ; Eva, Eva (referee)
This thesis deals with bank fees in the Czech Republic, which has been a topical issue in recent years. The objective of this thesis is to analyse and compare bank fees in the Czech Republic. It also aims to evaluate selected bank fees in terms of their importance for generating profit for the banks as well as from the clients perspective. The introduction to the theoretical part describes the basic characteristics of the banking system. The thesis then presents bank products and services, bank fees and the bank fee policy. The work includes a survey and analysis of the banking market in term of banking institutions in the years 2007 to 2014. It also includes a financial analysis for the years 2007 to 2015 and an analysis of the banking market from the perspective of the client, which compares selected banking institutions on the basis of charges and selected banking products based on fees. It defines three sample groups for which the most suitable banking products and banking institutions are recommended. In conclusion, the results are formulated together with recommendations for banks and clients. For the client Student came out best a current account UniCredit Bank, U Konto for youngsters. The Active client is also recommended by UniCredit Bank with banking product of U Konto. For target group Pensioner is the most suitable banking institution mBank where the monthly cost is CZK 70. The client can save thousands of CZK per year by making the right choice.
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Subsidy Support of the Sustainable Waste Management
BEDNÁŘOVÁ, Renata
The subject of the dissertation is the issue of the waste management with special emphasis on the recycling of the construction and demolition waste. The recourse is an analysis of the key instruments regulating the activities and behaviour of market operators in this field toward fulfilling all-society priorities and principles of the sustainable development, where special accent is put on the area of subsidy policies and programmes. The aim of the dissertation is to analyse and evaluate the setting of the selected subsidy programme in terms of economic and ecological efficiency with the emphasis on the assessment of the impact on the recipient. The subject of the research will be a priority axis 4 "Improvement of Waste Management and Removal of Old Environmental Burdens" within the Operational Programme Environment 2007 - 2013. In the literature review basic concepts and important definitions are defined, followed by the comprehensive and complex searches of the problematics from different angles of view. First of all, the global aspect of environmental protection is discussed, broader historical, legal, economic and political contexts, that are essential fundament for understanding the current and future challenges both globally regionally, are summarized. Hereafter, a more detailed description of the situation of the European Union is followed, then the overview is completed by a detailed view at the state of the problematics in the Czech Republic. Subsequent chapters are devoted to a detailed analysis of the waste management sector, again in terms of global, European and national standpoint, in the end the view is focused on the key issue of the dissertation problematics of construction and demolition waste. In the application part the fulfilling of the stated objective of the dissertation through four defined hypothesis is solved. Hypotheses verify the efficiency and appropriateness of the setting of the system of subsidies in the frame of the monitored programme. The emphasis is imposed on the assessment of the impact of the allocated subsidies to the private entities on their economic and environmental efficiency.
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Dmitry Shostakovich: Piano Trio Nr. 2 e-minor, op. 67
Škodová, Judita ; PETRÁŠ, Miroslav (advisor) ; ERICSSON, Mikael (referee)
The master thesis concerns with the Trio for violin, cello and piano Nr. 2 e minor by one of the most significant Russian composers of all times – Dmitry Shostakovich. Presenting a brief biography of the composer, the thesis continues introducing Ivan Ivanovich Sollertinsky, a very interesting personality of the Soviet cultural life and also one of the closest friends and colleagues of Shostakovich, to whom he dedicated his Trio Nr. 2. Following, there is a general introduction of the circumstances that influenced the composer’s life in those days, therefore also his musical language and the final shape of his Trio Nr. 2, including the circumstances of its premiere and also the most significant performers of this piece. The greatest part of the thesis concerns with an overal analysis of all the four movements of the composition from both technical and expressional point of view.
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Optimization of Gaussian Mixture Subspace Models and Related Scoring Algorithms in Speaker Verification
Glembek, Ondřej ; Brummer, Niko (referee) ; Campbell,, William (referee) ; Burget, Lukáš (advisor)
Tato práce pojednává o modelování v podprostoru parametrů směsí gaussovských rozložení pro rozpoznávání mluvčího. Práce se skládá ze tří částí. První část je věnována skórovacím metodám při použití sdružené faktorové analýzy k modelování mluvčího. Studované metody se liší převážně v tom, jak se vypořádávají s variabilitou kanálu testovacích nahrávek. Metody jsou prezentovány v souvislosti s obecnou formou funkce pravděpodobnosti pro sdruženou faktorovou analýzu a porovnány jak z hlediska přesnosti, tak i z hlediska rychlosti. Je zde prokázáno, že použití lineární aproximace pravděpodobnostní funkce dává výsledky srovnatelné se standardním vyhodnocením pravděpodobnosti při dramatickém zjednodušení matematického zápisu a tím i zvýšení rychlosti vyhodnocování. Druhá část pojednává o extrakci tzv. i-vektorů, tedy nízkodimenzionálních reprezentací nahrávek. Práce prezentuje dva přístupy ke zjednodušení extrakce. Motivací pro tuto část bylo jednak urychlení extrakce i-vektorů, jednak nasazení této úspěšné techniky na jednoduchá zařízení typu mobilní telefon, a také matematické zjednodušení umožněňující využití numerických optimalizačních metod pro diskriminativní trénování. Výsledky ukazují, že na dlouhých nahrávkách je zrychlení vykoupeno poklesem úspěšnosti rozpoznávání, avšak na krátkých nahrávkách, kde je úspěšnost rozpoznávání nízká, se rozdíly úspěšnosti stírají. Třetí část se zabývá diskriminativním trénováním v oblasti rozpoznávání mluvčího. Jsou zde shrnuty poznatky z předchozích prací zabývajících se touto problematikou. Kapitola navazuje na poznatky z předchozích dvou částí a pojednává o diskriminativním trénování parametrů extraktoru i-vektorů. Výsledky ukazují, že při klasickém trénování extraktoru a následném diskriminatviním přetrénování tyto metody zvyšují úspěšnost.
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