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The current state and prospects for sustainable development of tourism in Slovakia.
Libantová, Silvia ; Indrová, Jarmila (advisor) ; Chaloupka, Radek (referee)
Tourism contributes to the prosperity of destinations by bringing financial resources, new jobs, a higher level of services and infrastructure development. However, if tourism does not develop sustainably, it is accompanied by negative factors, as well. Especially, negative impacts on the environment, such as: water and soil contamination, increased noise and overcrowded destination during the high season. As a response to the worsening state of the environment and possible irreversible changes caused by irresponsible human behavior, the idea of sustainable development is becoming more important. The theoretical part describes tourism in the Slovak Republic and highlights plans for sustainable tourism development. In the practical part, the results of the questionnaire, related to the awareness of sustainable tourism in the Slovak Republic, are evaluated. The main objective of this thesis is to evaluate the current situation in the field of sustainable tourism and to make suggestions for its future development.

Changes in structure and phase composition in the surface of tram rail
Švábenská, Eva ; Roupcová, Pavla ; Schneeweiss, Oldřich
We have investigated structure and phase composition of surface layer of tram rails after long time running and the results were compared with those obtained on the original part of material. Changes due to effects of severe plastic deformation together with thermal shocks by friction process were expected. The information about structure and phase composition was obtained by optical and scanning electron microscopy, X-Ray Powder Diffraction, Mössbauer Spectroscopy and Glow Discharge Emission Spectroscopy (GDOES) and this was completed by microhardness measurements. The results show that the surface layer in comparison with the original material exhibits important changes in grain structure, an increase in microhardness and high content of iron oxide and hydrooxides. According to the depth profile of the chemical composition measured by GDOES there is an increase in carbon content in the surface layer which can be effect of up-hill diffusion.

A Comparison of Preconditioning Methods for Saddle Point Problems with an Application to Porous Media Flow Problems
Axelsson, Owe ; Blaheta, Radim ; Hasal, Martin
The paper overviews and compares some block preconditioners for the solution of saddle point systems, especially systems arising from the Brinkman model of porous media flow. The considered preconditioners involve different Schur complements as inverse free Schur complement in HSS (Hermitian - Skew Hermitian Splitting preconditioner), Schur complement to the velocity matrix and finally Schur complement to a regularization block in the augmented matrix preconditioner. The inverses appearing in most of the considered Schur complements are approximated by simple sparse approximation techniques as element-by-element and Frobenius norm minimization approaches. A special interest is devoted to problems involving various Darcy, Stokes and Brinkman flow regions, the efficiency of preconditioners in this case is demonstrated by some numerical experiments.

STRAIN ENGINEERING OF THE ELECTRONIC STRUCTURE OF 2D MATERIALS
del Corro, Elena ; Peňa-Alvarez, M. ; Morales-García, A. ; Bouša, Milan ; Řáhová, Jaroslava ; Kavan, Ladislav ; Kalbáč, Martin ; Frank, Otakar
The research on graphene has attracted much attention since its first successful preparation in 2004. It possesses many unique properties, such as an extreme stiffness and strength, high electron mobility, ballistic transport even at room temperature, superior thermal conductivity and many others. The affection for graphene was followed swiftly by a keen interest in other two dimensional materials like transition metal dichalcogenides. As has been predicted and in part proven experimentally, the electronic properties of these materials can be modified by various means. The most common ones include covalent or non-covalent chemistry, electrochemical, gate or atomic doping, or quantum confinement. None of these methods has proven universal enough in terms of the devices' characteristics or scalability. However, another approach is known mechanical strain/stress, but experiments in that direction are scarce, in spite of their high promises.\nThe primary challenge consists in the understanding of the mechanical properties of 2D materials and in the ability to quantify the lattice deformation. Several techniques can be then used to apply strain to the specimens and thus to induce changes in their electronic structure. We will review their basic concepts and some of the examples so far documented experimentally and/or theoretically.

Micro-electromembrane extraction across multiple aqueous and organic phases for selective pretreatment of raw biological samples
Kubáň, Pavel ; Boček, Petr ; Seip, K. F. ; Gjelstad, A. ; Pedersen-Bjergaard, S.
Micro-electromembrane extraction (μ-EME) across multiple aqueous and organic\nsolutions was developed for selective extractions of various analytes from untreated\nbiological samples. The novel approach requires μL volumes of samples and organic\nsolvents, which form consecutive immiscible plugs in a transparent polymeric capillary\nand the extraction process is accelerated by application of d.c. voltage. Number of the\nplugs, their composition, volume and sequence in the extraction capillary can be chosen\nspecifically for each particular application and high variability and selectivity of the\nextraction process can be achieved. In this contribution, we demonstrate suitability of\na five-phase μ-EME system for simultaneous extractions of cations and anions from\nurine and for selective extractions of basic analytes based on their acid-base strength\nfrom plasma.

Highly sensitive analysis of chlorophenols and sulfonamides in waters by electrophoretic focusion on inverse electromigration dispersion gradient with ESI-MS detection
Malá, Zdeňka ; Gebauer, Petr ; Boček, Petr
This work presents a new methodology for high-sensitivity analyses by capillary\nelectrophoresis (CE) with electrospray-ionization mass spectrometric (ESI-MS)\ndetection, based on electrophoretic focusing on an inverse electromigration dispersion\n(EMD) gradient. The technique is based on a robust electrolyte system providing a\ngradient of required properties, suitable for routine analyses of trace amounts of weak\nacids with pKa values between approx. 6.5 and 9. Examples analyses of several\nchlorophenols and sulfonamides are presented, with LODs around 3x10-9 M for spiked\ndrinking water without any sample pretreatment.

Investigation of bioactive proteins in pressurized water extract of Sambucus nigra L. branches
Hohnová, Barbora ; Šalplachta, Jiří ; Roth, Michal
Investigation of high-value substances in plant materials has been of increasing interest\nin last years. In this study, PHWE, SDS-PAGE and MALDI-TOF techniques were\nemployed for investigation of bioactive proteins in Sambucus nigra L. branches.

ELECTRON BEAM REMELTING OF PLASMA SPRAYED ALUMINA COATINGS
Matějíček, Jiří ; Veverka, J. ; Čížek, J. ; Kouřil, J.
Plasma sprayed alumina coatings find numerous applications in various fields, where they enhance the properties of the base material. Examples include thermal barriers, wear resistance, electrical insulation, and diffusion and corrosion barriers. A typical structure of plasma sprayed coatings, containing a multitude of voids and imperfectly bonded interfaces, gives them unique properties - particularly low thermal conductivity, high strain tolerance, etc. However, for certain applications such as permeation barriers or wear resistance, these voids may be detrimental.\nThis paper reports on the first experiments with remelting of plasma sprayed alumina coatings by electron beam technology, with the purpose of densifying the coatings and thereby eliminating the voids. Throughout the study, several parameters of the e-beam device were varied - beam current, traverse velocity and number of passes. The treated coatings were observed by light and electron microscopy and the thickness, structure and surface morphology of the remelted layer were determined and correlated with the process parameters. Based on the first series of experiments, the e-beam settings leading to dense and smooth remelted layer of sufficient thickness were obtained. In this layer, a change of phase composition and a marked increase in hardness were observed.\n

Geodiversity values as a basis for geosite and geomorphosite assessment: a case study from Žďárské vrchy Highland
Bajer, A. ; Kirchner, Karel ; Kubalíková, L.
Paper presents geodiversity values, geodiversity is understood as a set of geological, geomorphological and pedological components, including systems consisting of this components and geological, geomorphological and pedological processes. Geodiversity values are analyzed in relation to ecosystem values (e.g. cultural, functional value). Geodiversity values were used as basis for a methodical approach to geosite and geomorphosite assessment. Methodological approach is presented at selected sites in the top part of the Žďárské vrchy Highland. The acquired results can serve as a basis for geoconservation of PLA Žďárské vrchy, development of geotourism as well as an offer for extension geoeducational activities.\n

NUMERICAL SUPPORT FOR STUDY OF STRESS RATIO EFFECT ON FATIGUE CRACK BEHAVIOUR IN THREE POINT BEND SPECIMEN MADE FROM VIBRATED CONCRETE
Seitl, Stanislav ; Thienpont, T.
The fatigue behaviour of concrete has become more important for the design of structures due to more slender/slim structures, which are more sensitive to fatigue loading, or due to wind turbines, which are typically exposed to high-fatigue loading. The fatigue behaviour of concrete was investigated with respect to the influence of various stress ratios. Pilot analysis of fatigue crack propagation rate in three-point bend specimens made from ordinary vibrated concrete was done.