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Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Ledvina, Vojtěch ; Klepárník, Karel
Caspases are proteases that play key role in the process of apoptosis, the programmed\ncell death. Among them, caspase-3 and -7 are main executioner caspases that cleave\nmany vital proteins during apoptosis and after their widespread activation, the process\ncannot be reversed. To analyze caspase-3/7 activation within single cells, a miniaturized\ndevice for parallel analysis of eight samples was developed. The assay is based on the\nmodified luciferin-firefly luciferase bioluminescence (BL) system. Individual\nsuspended cells were collected and transferred into detection microvials using a\nmicromanipulator. The bioluminescence was detected using a photon counting head\nwith cooled photcathode. The LOD suitable for detection of active caspase-3/7 in both\napoptotic and non-apoptotic cells was reached.
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Intuition in creative process of animation movie
Nováčková, Jana Kristýna ; DĚCKÁ, Eliška (advisor) ; ČIHAŘOVÁ, Libuše (referee)
This work deals with the relation of emotions to contemporary experimental animation. It engages in the emotional experience of a viewer to non-narrative film storytelling and its means of expression.
At the same time, it examines what the creative processes and attitudes of filmmakers towards emotions in experimental animation are. Analysis of the questionnaires also reveals whether the creator counts on the viewer's specific interpretation of the film.
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The Role of a Nurse in Care of a Patient with Mononucleosis
LAŽANSKÁ, Soňa
Theoretical background Infectious mononucleosis belongs to relatively widespread viral infectious diseases in the Czech Republic. Epstein-Baar virus (EBV) participates in occurrence of the infection by 80 %, infectious mononucleosis may more rarely be caused by cytomegalovirus. Each individual factually suffers from infection caused by EBV during his/her life, however its course is unapparent in most cases. An affected individual or an individual not affected, who is a virus carrier may be considered the source of the infection. Infectious mononucleosis usually affects children and teenagers, in older individuals the infection is quite rare. The disease might start rapidly or gradually with prodromes like headache, sore throat, nausea, fatigue and perspiration. Infectious mononucleosis is often confused with other diseases like e.g. tonsillitis, or more serious progress of flue, particularly for the symptoms accompanying the disease. Fever, lymphadenopathy and hepatosplenomegaly are the most frequent symptoms. The disease may also cause numerous unpleasant complications, particularly hematologic, neurologic, cardio-pulmonary and autoimmunity complications. A nurse plays a very substantial role in client care. It is very important for a nurse to have sufficient knowledge and to know the right procedure of all interventions he/she meets within diagnostics and treatment of infectious mononucleosis. These are particularly biological material collection, per os administration or intravenous administration of medicines. A nurse should also be familiar with the Priessnitz compress application technique. In non-self-sufficient clients a nurse is also responsible for client's hygiene. A nurse has an important role in client education providing information on dietetic measures. A nurse has to lead a client to adherence to the prescribed diet and body calm. Everything depends on client's current condition. A client should also avoid mental strain during convalescence. We should note that not all forms of infectious mononucleosis require hospitalization. The aim of the thesis The aim of the thesis was to find out the role of nurses in the care of patients with infectious mononucleosis and also to find how satisfied patients with the nursing care and education are after going through mononucleosis. This was why the following research questions were chosen: What is the role of a nurse in care of a patient with mononucleosis? How satisfied are patients with the nursing care after going through mononucleosis? How satisfied are patients with education in diet regime adherence after going through mononucleosis? Applied Methods Qualitative research was chosen for finding out the required information. The information was gained by means of deep interviews with nurses working at the infectious department of České Budějovice Hospital and with clients after going through infectious mononucleosis. All the interviews were recorded on a voice recorder and then transcribed. The transcribed interviews were subject to detailed analysis by the paper and pencil method with open coding. Results Six categories were set upon analysis of the interviews with nurses: Awareness of the infectious mononucleosis disease among nurses, Nursing care of a client with infectious mononucleosis, Regime measures, Information on dietetic measures, Recommendations after infectious mononucleosis and Education of a client with infectious mononucleosis. Five categories were set upon detailed analysis of the interviews with the clients: Recommended treatment, Nursing care, Recommended measures, Methods of information communication and Information sources for clients. Subcategories, in which the key data are coded, are assigned to the individual categories.
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Neural Networks Between Integer and Rational Weights
Šíma, Jiří
The analysis of the computational power of neural networks with the weight parameters between integer and rational numbers is refined. We study an intermediate model of binary-state neural networks with integer weights, corresponding to finite automata, which is extended with an extra analog unit with rational weights, as already two additional analog units allow for Turing universality. We characterize the languages that are accepted by this model in terms of so-called cut languages which are combined in a certain way by usual string operations. We employ this characterization for proving that the languages accepted by neural networks with an analog unit are context-sensitive and we present an explicit example of such non-context-free languages. In addition, we formulate a sufficient condition when these networks accept only regular languages in terms of quasi-periodicity of parameters derived from their weights.
Fulltext: content.csg -  PDF
Plný tet: v1237-16 - PDF
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Natural houses in gardens
Formánková, Kristýna ; Kunt, Miroslav (advisor)
Thesis discusses natural houses which are supposed to be part of our lives in the future. These houses may be for someone and they might prefer smarter building with non-recyclable materials when choosing a new housing. But it is necessary to think about our future and sustainable development. We also need to tell ourselves what is really a priority for our planet. There comes a time when they will prefer building materials which can be used somehow after some time or be recycled. This is related to the design of the garden. It is designed to fulfil an ornamental, economic and social function. The garden is an integral part of each house, therefore it is important for each element used to be creating the garden as a linked harmonious whole. The study deals with the concept of a natural houses home in accordance with the maximum use of the garden.
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Effect of snowpack on runoff generation during rain on snow event.
Juras, Roman ; Máca, Petr (advisor) ; Ladislav , Ladislav (referee)
During a winter season, when snow covers the watershed, the frequency of rain-on-snow (ROS) events is still raising. ROS can cause severe natural hazards like floods or wet avalanches. Prediction of ROS effects is linked to better understanding of snowpack runoff dynamics and its composition. Deploying rainfall simulation together with hydrological tracers was tested as a convenient tool for this purpose. Overall 18 sprinkling experiments were conducted on snow featuring different initial conditions in mountainous regions over middle and western Europe.
Dye tracer brilliant blue (FCF) was used for flow regime determination, because it enables to visualise preferential paths and layers interface. Snowpack runoff composition was assessed by hydrograph separation method, which provided appropriate results with acceptable uncertainty. It was not possible to use concurrently these two techniques because of technical reasons, however it would extend our gained knowledge. Snowmelt water amount in the snowpack runoff was estimated by energy balance (EB) equation, which is very efficient but quality inputs demanding. This was also the reason, why EB was deployed within only single experiment.
Timing of snowpack runoff onset decrease mainly with the rain intensity. Initial snowpack properties like bulk density or wetness are less important for time of runoff generation compared to the rain intensity. On the other het when same rain intensity was applied, non-ripe snowpack featuring less bulk density created runoff faster than the ripe snowpack featuring higher bulk density. Snowpack runoff magnitude mainly depends on the snowpack initial saturation. Ripe snowpack with higher saturation enabled to generate higher cumulative runoff where contributed by max 50 %. In contrary, rainwater travelled through the non-ripe snowpack relatively fast and contributed runoff by approx. 80 %.
Runoff prediction was tested by deploying Richards equation included in SNOWPACK model. The model was modified using a dual-domain approach to better simulate snowpack runoff under preferential flow conditions. Presented approach demonstrated an improvement in all simulated aspects compared to the more traditional method when only matrix flow is considered.
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Design of Experiment for Non-Stationary Processes of Production
Jadrná, Monika ; Macák, Tomáš (advisor)
The doctoral thesis is concerned with the services sector and the area of mass production. Particularly, the optimization of the product portfolio of the travel agency and the optimization of production rounds of ammunition.
The theoretical part deals with the current overview of discussed topic. Further, the terminology and methods of the decision-making process are defined to support decision making. The theoretical basis of research focused on the choice of appropriate input variables in the area of services, and on the choice of a particular material option in the production area and appropriate equipment for the production. The theoretical part forms the basis for the practical part of the thesis.
For the doctoral thesis was chosen an enterprise operating in the defined sector. Product portfolio for the services sector is optimised using Fuzzy logic and Fuzzy sets so that the enterprise can maintain its competitiveness in todays highly ambitious market. Product portfolio for manufacture is optimised for achieving desired properties of the product.
The main aim of the thesis is to propose a new methodological approach for the management of selected business processes in their nonstationary time course. The aim of the practical implementation is to verify the functionality of the proposed methodological approach, both in the area of services and in the field of mass production.
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Non-invasive methods for sex and steroid homones determination in gibbons of the genus Nomascus
Bolechová, Petra ; Bartoš, Luděk (advisor) ; Pavel, Pavel (referee)
The gibbon primates of the family Nomascus are classified as critically endangered species, and, to date, basic understanding and information about their biology is missing. With regard to the status of these animals in the wild and captive populations in zoos, being familiar with their reproduction, may improve captive breeding programs. Data collection in this study was to be carried out by the practical use of non-invasive methods (polymerase chain reaction for DNA extraction and enzyme immunoassays), using faecal samples for analysis. The first method, polymerase chain reaction, was used for sex determination in juveniles, because of their coat colour and visual similarity of secondary sex characteristic in both sexes; it is not possible to determinate sex without handling the animal. Another main purpose of this study is to try and answer the hypothesis regarding the ovarian cycle of females, factors influencing their hormone concentration and also the onset of sexual maturity in females and the timing of their fur colour change. Hypotheses were checked by monitoring the concentration of progesterone and oestrogen faecal metabolites and by evaluation of the composition of breeding groups of gibbons with the ZOO influence. During a four year period (from 2010 till 2014), there were a total of 51 animals analysed from 16 different zoos with a faecal sample count totalling 1618 samples. The results confirmed the use of noninvasive methods for sex determination, and thus ensuring the maximum welfare standards. Endocrinological analysis confirmed the hypothesis of the influence of the environment (ZOO) to the hormone concentrations and female´s pregnancy effect in conjunction with her age on the final results of the male - father hormone concentrations. The initial information in this study is the confirmation of the onset of ovarian cycle in young females without connection to their fur colour change and a significant factor of a mother´s impact and her territoriality. This study is the first to present a long term monitoring of ovarian cycles in females and hormone concentrations of other individuals, both male and female, and from various age groups. The results allow us to understand the possible impact of zoo environments on the reproductive status of these gibbons and contribute to the general improvement of breeding management.
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DCTOOL-A3
Bakule, Lubomír ; Papík, Martin ; Rehák, Branislav
DCTOOL-A3 is a documentation of Matlab routines developed for the design of decentralized control of large scale complex systems. The current beta version covers three areas as follows:\nReport 4.1 deals with the event-triggered control design for unstructured uncertain systems. Both non-quantized and quantized feedback is analyzed. The results are given in terms of linear matrix inequalities (LMIs). Logarithmic quantizer is used. Numerical example illustrates the effectiveness of the presented results.\nReport 4.2 presents a new decentralized overlapping wireless control design with a switched communication protocol. The method is applied by simulations on the 20-story building structure including the test of robustness of the methods against sensor failures and network node dropouts of a digital network.\nReport 4.3 presents the construction of a new decentralized wireless controller and a set of heuristic algorithms for evaluation of packet dropouts, sensor faults and actuator faults. The digital network operates at the standard frequency used in well-known widely-used industrial protocols. The results are tested at the Benchmark model decomposed into two disjoint substructures. The results are published. Thus, the details are omitted here.
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