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Properties of aminoclay complexes and biologically active substances
Dušek, Jakub ; Pekař, Miloslav (referee) ; Mravec, Filip (advisor)
This paper builds on previous research of aminoclay complexes in undergraduate studies. Theoretical part deals with study of current problems of aminoclay complexes with bioactive substances and the choice of substances for complexing with aminoclay. The experimental part consists of preparation of aminoclay complexes with selected bioactive agents at various concentrations. Verification of binding of bioactive agents to the aminoclay matrix was performed by Elemental Analysis (EA) and Fourier-transform infrared spectroscopy (FT-IR). For finding of the bound amount of bioactive substance were used the Ultra Performance Liquid Chromatography (UPLC®) and analysis of the cytotoxic properties of the formed complexes by used by the MTT assay. The main motivation of this study is to create new complexes with improved characteristics that would replace existing forms of substances used in pharmaceutical and biomedical applications.
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Analysis of biologically active substances in apricot oil
Valasová, Denisa ; Márová, Ivana (referee) ; Němcová, Andrea (advisor)
The Master’s thesis is focused on the analysis of biologically active substances in apricot kernel oil. Apricot oil has recently begun to be widely used in the cosmetics industry, particularly as a part of massage oils or as part of moisturizing creams. The aim of this thesis is to characterize and assess the quality of apricot oil, determination of the content of selected active ingredients, through a series of analytical methods, and subsequent use of this oil in cosmetics and food industry. The thesis is based on the comparison of the quality of pure apricot oil and the oil after the fermentation process. It has been found that apricot kernel oil actually contains large amounts of unsaturated fatty acids. The excellent antioxidant activity of apricot kernels was proven too, especially those after fermentation. In addition to antioxidant activity, the samples contain a relatively large amount of tocopherol after the fermentation. Tocopherol is important for the uptake of free radicals in lipid bilayers, thus slowing down aging, protect the membranes and also prevent from possible cardiovascular and oncological diseases. Furthermore, in the Master’s thesis there were confirmed not only the hydration effects of creams with apricot oil, but also their ability of inhibic transepidermal loss of water from the epidermis. Creams were tested on five probands, aged 23–24, with different skin types. After the application of creams to their skin, these properties greatly improved, the skin has been regenerated and hydrated for 24 hours after the treatment.
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Monitoring of vitamin E content in the samples of cereals
Cibulcová, Pavla ; Borkovcová,, Ivana (referee) ; Benešová, Karolína (advisor)
Thesis deals with the analysis of vitamin E in cereal samples, namely barley and wheat and malt produced from them. Selected samples were treated with low-temperature plasma, fungicide and biological treatment to elimination fungus and mycotoxins. The aim of the thesis was to find out the influence of the treatment, the combination of several types of treatment on vitamin E content in grain and malt. If the content of health-positive substances is not reduced and grain can be used for food, malt, or for feed purposes. The theoretical part focuses on the properties, occurrence, biochemistry and importance of vitamin E for living organisms. It also focuses on the properties of cereals that are a significant source of vitamin E. Malt production and sample treatment are also described. The experimental part describes the field experiment, individual types of treatment, extraction of vitamin E from the samples and determination of vitamin E content by high performance liquid chromatography with fluorescence detection. The results are compared with the current literature. It was found that the treatment did not have a significant negative effect on vitamin E content.
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