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Role cytokinů ve vývoji a diferenciaci regulačních T buněk
Procházková, Jana ; Holáň, Vladimír (advisor) ; Kovář, Marek (referee) ; Stříž, Ilja (referee)
The development and function of T helper (Th) cells and regulatory T cells (Tregs) are plastic processes that are regulated by cytokines. In our project we first analyzed the effect of different cytokines on the development of induced (i) Tregs. It has been demonstrated that iTregs arise from CD4+ CD25- T cells upon stimulation with alloantigen in the presence of transforming growth factor β (TGF-β). The development of these Tregs and their proliferation were inhibited by interleukin (IL)-4 and IL-12. The aquired results also demonstrated distinct responses of naturally occuring (n) Tregs and iTregs to the regulatory action of IL-4 and an opposite role of IL-4 in maintenance of nTregs and iTregs phenotype. An important role in the induction of T cell subsets may play also mesenchymal stem cells (MSCs) which can, under specific conditions, produce TGF-β and IL-6. Depending on the current production of TGF-β or IL-6, MSCs can qualitatively regulate the ration between Tregs and Th17 cells. Anti-inflammatory Tregs and pro-inflammatory Th17 cells are induced upon stimulation in the presence of TGF-β and TGF-β and IL-6, respectively. In addition to our previous work we studied the role of IL-12 in the development of Tregs and Th17 cells. It was shown that Treg and also Th17 cell differentiation was...
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Hereditary immunological diseases in dogs
KREJČOVÁ, Lenka
This thesis summarizes the most important findings of primary immunodeficiencies in different breeds of dogs, their genetic background, symptoms, their treatment options: canine leukocyte adhesion deficiency, trapped neutrophil syndrome, Pelger-Huet anomaly, severe combined immunodeficiency, grey collies syndrome, selective IgA deficiency, complement C3 deficiency and growth hormone deficiency with hypoplastic thymus in dogs. This diseases, often with autosomal recessive inheritance, are for affected individuals usually fatal. Research of primary immunodeficiencies in dogs is substantial also because it often serves as a model for similar studies human deficiencies.
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