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Ovlivnění stability šťávy zeleného ječmene pomocí obsahových látek z vybraných rostlinných druhů
Juříčková, Jana
The thesis „Affecting the stability of the juice of green barley using the content substances of selected plant species“ is usage of green barley substances, the green barley juice production and its stabilization with various plant extracts. In the theoretical part the aim is to define young green barley, important substances in the plant and how to obtain them, which plant extracts and preservation methods are used in barley juice production. The practical part of this thesis is about cultivating green barley in laboratory conditions, its processing with plant extracts addition and the effeciency monitoring for 3 weeks. Plant extracts have antimicrobial activity against various microorganism, and so the extracts are suitable for juice preservation. Moreover the extracts help to preserve the nutrients in the juice.A microbiological risk assessment was performed on a samples with the plant extracts and a microbiological evaluation was done in a stastistical analysis software. The result from the microbiological evaluation the most effective against microorganisms was Silybum m. extract.
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Vyhodnocení aplikace elicitorů na zdravotní stav révy vinné
Šmíd, Roman
This Masters thesis contains possibility in using plant extracts as alternative options for protecting grapevine against fungal diseases. Also Impact using of water plant extracts to grapevine state of health and their quantity and qualitative parameters. Theoretic part of this masters thesis describe the most significant fungal diseases of grapevines. In following article are described manufacturing possibilities and usefulness of plant extracts. Plants convenient to produce extracts. The last paragraph in theoretical part contains characterization of defending reactions of grapevines, attacked by pathogens. Beginning of experimental part deals with influence of plant extracts to grapevine state of health. There is also comparison followed parameters between all tested variants.
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Porovnání kvantitativních a kvalitativních vlastností odrůdy Ryzlink rýnský v ekologické a integrované produkci
Juga, Petr
This diploma thesis are focused on the comparison of integrated and organic production. The literary part deals with the problems of production systems in viticulture and explain their influence on the quality of grapes. It also describes plant extracts, used plants and their production. It describes the main fungal diseases that the vines invade. The experimental part describes the experiment itself, the plant protection products used and the methods used to evaluate the quantitative and qualitative parameters of Riesling grapes that have been observed during maturation. It deals with the evaluation and comparison of the monitored parameters. The thesis recommends an appropriate system of cultivation in organic and integrated production.
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Analysis of active substances contained in lavender and thyme extracts
Vašíčková, Milada ; Vysoká, Marie (referee) ; Skoumalová, Petra (advisor)
The presented bachelor thesis is focued on active substances contained in lavandula officinalis and common thyme extracts, mainly their possible antimicrobial and antioxidant effects. The theoretical part desrcibes chosen plants, compounds with antimicrobial and antioxidant effects, methods how to obtain and identify said compounds. In the experimental part, aquenous, ethanolic and oil extracts were prepared. Mentioned extracts were spectrophotometrically characterised for total content of polyphenols, total content of flavonoids and antioxidant activity. In conclusion possible applications of extracts in food industry instead of synthetic preservatives and their fotification were discussed.
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Preparation of bioactive wound dressings and testing their interaction with human cells
Bendová, Agáta ; Márová, Ivana (referee) ; Skoumalová, Petra (advisor)
The thesis was focused on the preparation and optimization of the preparation of wound dressing from materials with bioactive ingredients. In this work were prepared nanofiber dressings based on polyhydroxybutyrate and non-fibrous dressings from alginate and chitosan. Nanofibers were prepared by electrospinning and forcespinning methods. The bioactive components, which were used to functionalize the prepared dressings, were plant extracts, clotrimazole, ampicillin, lysozyme, and proteolytic enzymes. The theoretical part is focused on the description of the use of nanofibrous and non-fibrous materials in medicine, characterization of materials for the production of wound dressings and bioactive components. Furthermore, this section describes the methods used to prepare and characterize wound dressings. In the practical part were prepared aqueous and oil extracts from selected plants. Extracts were characterized for polyphenols content and antioxidant activity. PHB-based nanofibers were prepared using electrospinning and forcepinning methods. Nanofibers were enriched with selected plant oil extracts and clotrimazol. Modified nanofibres were detemined for antioxidant activity, short-term and long-term stability. Non-fibrous wound dressings were prepared from alginate and chitosan. These dressings were functionalized by the addition of selected aqueous extracts, ampicillin, lysozyme, papain, bromelain, and collagenase. Non-fibrous wound dressings were determined for antioxidant activity, short-term stability and proteolytic activity. The prepared wound dressings were tested for their antimicrobial effects on cultures of Micrococcus luteus, Serratia marcescens, Staphylococcus epidermidis and Escherichia coli. In conclusion, successfully prepared bioactive wound dressings with antioxidant and antimicrobial agents were tested for safety on human cells. The determination was performed using the MTT cytotoxicity test on human keratinocytes.
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Study of biological effects of some plant materials
Drabíková, Nela ; Byrtusová, Dana (referee) ; Márová, Ivana (advisor)
The presented bachelor thesis deals with the preparation of aqueous, ethanolic and hexane extracts obtained from plant sources with increased content of oil, their characterization and cytotoxicity testing. In the theoretical part, a review focused on basic information about the used plants and their effects on the human body was elaborated. Furthermore, the active ingredients of the extracts, methods of their determination and the principle of the cytotoxicity test were described. In practical part the content of polyphenols and flavonoids was determined spectrophotometrically. Further, total antioxidant activity was measured. The fatty acids composition and the percentage of individual fatty acids were determined by gas chromatography. Next, MTT cytotoxicity assay was performed using human cell culture to confirm the safety of extracts for potential use in food, cosmetic and pharmaceutical industries.
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Determination of rubidium in selected plant extracts by atomic absorption spectrometry
Šatrová, Lucie ; Hraníček, Jakub (advisor) ; Jelínek, Ivan (referee)
In this diploma thesis, rubidium in plant material samples was determined by atomic absoption spectrometry. Determination of plant material rubidium was performed on two different atomic absorption spectrometers (GBC 933 AA and ContrAA 700) for comparison. The selection of a proper method of atomization was essential, therefore optimizations for the flame atomizer and electrothermal atomizer were performed. On the GBC 933 AA, flame atomization was tested. The flow rate of the acetylene-air, vertical and horizontal flame profile, spectral interval width was optimized for the instrument. On the ContrAA 700, the conditions for flame atomization were optimized as well as for electrothermal atomization. The optimized parameters included the acetylene-air flow rate and the vertical flame profile again. For the electrothermal atomization, the temperature dependence of pyrolysis and the temperature dependence of atomization were optimized. Under experimentally determined optimal conditions, the determination of rubidium in fruit and vegetable juice samples was performed by the method of calibration curve. Rubidium usually accompanies toher alkali metals. In the absence of essential biogenic elements iportant for plant growth, rubidium is able to help out and take on the role of potassium.
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Vliv aplikace rostlinných extraktů na zdravotní stav PIWI odrůd
Radkovský, Filip
This thesis focuses on the protection of vine against fungal diseases. Application of alternative means in the form of aqueous extracts from ordinary available plant of their effect on health status Piwi varieties. The theoretical part of this thesis describes the most important fungal diseases of grapes. Further they are described interspecific varieties, which were used for the experiment. Literary part is devoted to the preparation of herbal liquors and extracts of herbs and their active substances in a plant extracts. The experimental part is devoted to the influence of herbal preparations on the health of Piwi varieties. Results were statistical and analytical evaluated. Monitored parameters were comparison with all tested variants.
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Testování antimikrobiální aktivity obalových materiálů s rostlinnými extrakty proti E. coli a koliformním bakteriím
Šabršula, Jan
This dissertation deals with evaluation and testing of food packiging materials antimicrobial aktivity that contain plant extracts and help in the fight against Eschericia coli and other coliform bacteria. This dissertation is divided into several parts. The first half of the work contains a literature review publishing about packaging materials containing plant extracts. It is also focused on literature review on the coliform bacteria, especially Escherichia coli genus, describes these microorganisms and their metabolic products, the effects of metabolic products which may negatively affect human health. Another part deals with measures to prevent undesirable microorganisms. The second half of the work has been engaged in actual testing of packaging materials containing plant extracts on relevant model organisms or directly on food. Data, acquired by testing are processed, compared with the figures in the available literatures and are listed at the end of work.
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