|
|
Homogenized illumination source for microscopy
Riznerová, Petra ; Slabý, Tomáš (referee) ; Dostál, Zbyněk (advisor)
This thesis deals with the design of an illumination system for homogeneous illumination of the sample of a coherence-controlled holographic microscope. The theoretical part of the thesis briefly describes the principle of interference and holographic microscopy in connection with the use of a suitable illumination system for microscopy, including methods of light homogenization. The definition of the parameters of the new miniaturized source is based on this part. The work includes two designs of an illumination system with different use of microlens arrays. Optical simulations are performed, which verify the correct selection of all optical elements of the illumination system. Following the optical models, optomechanical designs are created. These are compiled and tested. The light efficiency of the sources and the distribution of light intensity values in their output planes are measured.
|
|
|
Internet Educational Atlas Focusing on Soil Arthropods
Dvořáková, Jana ; Mourek, Jan (advisor) ; Říhová, Dagmar (referee)
The main objective of this thesis was to create an educational website aimed at soil arthropods. It can be used primarily by teachers of biology and ecology, lecturers of extracurricular science education or by the students who are interested in this issue. This photographic atlas of soil arthropods consists of my own micro images obtained by the use of light and scanning electron microscope. The micro images are accompanied by text to each group of soil arthropods, which is divided into sections containing information about the systematic classification, anatomy and morphology, biology and importance, eventually representatives of the group. The atlas is accompanied by other materials usable in the classroom, such as didactic test, didactic game and proposals for group work, worksheet, field and laboratory work or educational presentations focused on soil arthropods. These materials can be downloaded from the website, along with the entire atlas of soil arthropods. The atlas is available on the following address: https://sites.google.com/site/pudniclenovci/. The review of the literature deals with the importance of arthropods in the soil and their mutual interactions. Then I describe the methods of study of arthropods (sampling, sample preparation for light and scanning electron microscopy and...
|
|
| |
|
|
Microscopy: tool for material analysis
Pleskalová, Kateřina ; Řehořek, Lukáš (referee) ; Čupera, Jan (advisor)
This thesis deals with description of the microscopic techniques that are used to analyse materials. The introductory part briefly summarizes the history of microscopy. The first part of the thesis is focused on light microscopy and the confocal microscopy technique is more elaborated here. The second part of the thesis is focused on basic techniques of electron microscopy, which means transmission electron microscopy and scanning electron microscopy. At the end of each chapter, there is summarization of limits and advantages of these techniques. The preparation of metallographic samples is also briefly described for these microscopic techniques.
|
|
| |
|
|
Novel cancer biomarkers derived from quantitative phase imaging of biopsy cells
Plišková, Diana ; Týč, Matěj (referee) ; Kolářová, Jana (advisor)
The main objective of this work is the development of novel cancer biomarkers usable in personalized treatments. To understand why this issue is important, a brief description of cancer, including statistical results over the past years, is provided. The work also describes individual methods of light microscopy that can be used in cell analysis and subsequent image processing consisting of segmentation, tracking, feature extraction and classification. In this work, the main cell features, such as cell motility and shape, are presented. These features can be potential biomarkers in the treatment of cancer.
|
|
|
Heat treatment of iron alloys with carbon
Kubíček, Antonín ; Štěpánek, Roman (referee) ; Čupera, Jan (advisor)
This thesis deals with the processes of heat treatment, their impacts on microstructure of iron alloys with carbon and their influence on hardness of these alloys. In the research part of this thesis are discussed the alloys, which are found within the Fe-Fe3C metastable system, principles and technologies of heat treatment processing with focus on the annealing, methods of microstructure examination and hardness measurement. In the experimental part is documented and evaluated the influence of various types of annealing on hypoeutectoid and hypereutectoid steels and the influence of graphitization on the hypoeutectic cast iron. The evaluation involves comparison of microstructures obtained by light and scanning electron microscopy and comparing the hardness in the green state and after annealing.
|
|
|
Tapeworm parasitic in the digestive tract rays Amblyraja radiata on Svalbard
PADALÍKOVÁ, Petra
This bachelor thesis is interested in tapeworms of the stingray Amblyraja radiata. These rays Ambyraja radiata living in Svalbard. It is known about 5000 species of parasitic tapeworms which are parasites on vertebrates. The highest number of tapeworms lives in cartilaginous fish, and fish. Adults are found in the digestive system of vertebrates. In obtained samples were found three genera of tapeworms: Grillotia, Pseudanthobothrium, Echeneneibothrium. My objective was to determine the species. My aim was to compare the results with the literature and characterize the community tapeworm rays Amblyraja radiata in Svalbard. Identification of tapeworms was based on morphological characters. Morphological features are (eg. strobili maximum width, length cirrus sac, testes number and shape of hooks.) These characters I visualized by means of staining, light microscopy and electron microscopy. I used a carmine staining into individual samples tapeworms. Thanks to this coloring I achieved better visibility of internal organs, particularly genitals. Thanks to scanning electron microscopy I was allowed to observe the specimen surface.
|
|
|
Image Analysis in Microscopy and Videokymography
Sedlář, Jiří ; Flusser, Jan (advisor) ; Jan, Jiří (referee) ; Cristobal, Gabriel (referee)
13551398399831-171f83a418df200fec6f622f22b6fb3d.txt The Thesis describes new methods for automatic processing of image data in biology, physics and medicine. The developed methods reconstruct light microscopy images of growing microorganisms in intervals between observations, measure particles in atomic force microscopy images, and evaluate parameters of vocal fold vibrations in videokymographic images. All three problems have been hitherto solved primarily visually. The proposed methods allow automatic or computer-aided processing of the image data, and thus facilitate the evaluation process. Performance of the developed methods was tested on real images; the results were comparable with ground truth or results of visual evaluation. Application of the developed methods is not limited to the specific type of image data; the methods can be used in general for processing of images with similar characteristics. Page 1
|
|
|
Internet Educational Atlas Focusing on Soil Arthropods
Dvořáková, Jana ; Mourek, Jan (advisor) ; Říhová, Dagmar (referee)
The main objective of this thesis was to create an educational website aimed at soil arthropods. It can be used primarily by teachers of biology and ecology, lecturers of extracurricular science education or by the students who are interested in this issue. This photographic atlas of soil arthropods consists of my own micro images obtained by the use of light and scanning electron microscope. The micro images are accompanied by text to each group of soil arthropods, which is divided into sections containing information about the systematic classification, anatomy and morphology, biology and importance, eventually representatives of the group. The atlas is accompanied by other materials usable in the classroom, such as didactic test, didactic game and proposals for group work, worksheet, field and laboratory work or educational presentations focused on soil arthropods. These materials can be downloaded from the website, along with the entire atlas of soil arthropods. The atlas is available on the following address: https://sites.google.com/site/pudniclenovci/. The review of the literature deals with the importance of arthropods in the soil and their mutual interactions. Then I describe the methods of study of arthropods (sampling, sample preparation for light and scanning electron microscopy and...
|
guest ::
