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Reconstruction methods in holographic microscopy
Findejsová, Anna ; Odstrčilík, Jan (referee) ; Kolář, Radim (advisor)
This master’s thesis focuses on the image reconstruction from holographic microscope. The first part of the thesis summarizes problems of holography, describes its principle and application particularly in live cell biology. In the second part the description of several methods used for off-axis hologram reconstruction is provided. The last part describes the implementation of the basic step in holographic reconstruction – the elimination of autocorrelation and twin image – and then also the reconstruction of 3D information of the sample.
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Fresnel Incoherent Correlation Holography (FINCH)
Bouchal, Petr ; Slabý, Tomáš (referee) ; Chmelík, Radim (advisor)
In the Bachelor's thesis, the recently proposed method known as Fresnel Incoherent Correlation Holography (FINCH) is examined both theoretically and experimentally. Its main advantage consists in a possibility to realize holographic reconstruction of 3D objects illuminated by incoherent light. In FINCH, the object recording is performed applying methods of optical holography and digital diffractive optics. The object reconstruction is realized numerically and utilizes principles of digital holography. In experiments, the modern optoelectronic devices known as Spatial Light Modulators are effectively used. The Bachelor's thesis includes a short review including description of the basic principles of FINCH but its own contribution consists in the mathematical description of the method and creation of the numerical simulation model in Matlab. The main result of the thesis is design and realization of experiments enabling verification of the method. In the Bachelor's thesis, results of two independent experiments realized with different types of Spatial Light Modulators HOLOEYE and HAMAMATSU are presented. An agreement of experimental results with theoretical predictions is very good. A short discussion of the obtained results, further research topics and FINCH applications is also included in the Bachelors's Thesis.
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Optical Field Evaluations for Hologram Synthesis
Fedorko, Matúš ; Polok, Lukáš (referee) ; Zemčík, Pavel (advisor)
The primary concern of this work is computer generated holography, in particular accelerating computation of synthetic holograms by means of modern-day massively parallel graphics chips. It starts by introducing fundamental holography principles together with mathematical and physical concepts behind it. Following discussion then deals with OpenCL, which is a relatively new, but well recognized standard for GP-GPU programming. Finally, the last two chapters of this work give an overview of problem analysis and its implementation in C++.
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Three-Dimensional Reconstruction of Image in Digital Holographic Microscopy
Týč, Matěj ; Karásek,, Vítězslav (referee) ; Martišek, Dalibor (referee) ; Chmelík, Radim (advisor)
This thesis deals with the topic of 3D image processing for digital holographic microscopy - numerical refocusing. This method allows to perform mathematically accurate defocus correction on image of a sample captured away from the sample plane and it was applicable only for images that were made made using coherent illumination source. It has been generalized to a form in which it is also applicable to devices that use incoherent (non-monochromatic or extended) illumination sources. Another presented achievement concerns hologram processing. The advanced hologram processing method enables obtaining more data mainly concerning precision of quantities from one hologram — normally, one would have to capture multiple holograms to get those. Both methods have been verified experimentally.
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Synthetic Holography
Tvarog, Drahoslav ; Chmelík, Radim (referee) ; Kotačka, Libor (advisor)
The thesis presents method of a creation of computer-generated holograms. The first part is devoted to an introduction of theoretical principles of a generation of synthetic holograms. The second part of the discourse deals with several practical methods of synthetic holograms creation. We present a computer code computing a discrete Fourier transform of an input image. The output of the code is an image matrix binarized in a specifically given way and printed on a transparent slide. When reconstructing the synthetic hologram we initially illuminate the slide with CGH by a plane wave and a reconstructed pattern is magnified through a camera objective to obtain a pattern observable by naked eyes. Finally, we analyze the quality of reconstructed pictures obtained from the synthetic holograms.
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3D Game Exploiting Focusing on Holographic Display
Lisický, Tomáš ; Materna, Zdeněk (referee) ; Chlubna, Tomáš (advisor)
This thesis is anylising the possibilities of 3D display in gaming environment, specifically 8.9'' model of the first generation by Looking Glass company. The main emphasis is put on the usage of the given display's focusing, thus in which game mechanics and environments it is usable with desired effect on player and his perspective sense. The thesis' purpose is also to survey the market of available and historical games, which use 3D displays or other stereoscopic elements for visualisation or other purposes. After survey comes analysis of found game genres and their game mechanics, evaluation of their usage, performance and the option of implementation on Looking Glass display. The final product is a short demo of 3D game, which uses these game mechanics in combination with Looking Glass' focusing capabilities. To realise connection between this display and gaming environment, an open-source development platform for PC games – Unity – was used.
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Non Realistic Visualization on Lume Pad
Chalivopulosová, Aneta ; Karas, Matej (referee) ; Milet, Tomáš (advisor)
This bachelor's thesis focuses on methods of non-realistic rendering on Lume Pad. This thesis aims to test the functionality, quality, and user-friendliness of a few selected non-realistic effects applied to 3D scenes on the holographic tablet Lume Pad. These non-realistic post-processing effects are implemented and described in this thesis. Namely, edge detection, ordered dithering, random dithering, thresholding, hatching, color shading, wobbling effect and wobble carving effect, and pencil effect. The text also describes a theoretical introduction to holography, stereoscopy, and the light field.
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Fiber guided illumination module
Kropáč, Vlastimil ; Kvasnica, Lukáš (referee) ; Dostál, Zbyněk (advisor)
This diploma thesis describes the design of the illumination system for a Coherence--Controlled Holographic Microscope (CCHM). The theoretical part mentions the history of microscopy, the principle of holography and individual types of interference microscopy. To get closer to the topic, individual light sources and an overview of current illumination systems are mentioned. The diploma thesis also describes the procedure of designing a fiber-optic illumination module for microscopy from optical design through design of construction to the last step, which is assembly and testing of the module.
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Phase imaging below the diffraction limit
Nečesal, Daniel ; Bouchal, Zdeněk (referee) ; Dvořák, Petr (advisor)
Tato diplomová práce se zabývá konstrukcí interferenčních zařízení pro výzkum měření fáze světla na mikro-strukturách a fáze plazmonů na nano-strukturách. V první kapitole je vybudován teoretický základ pro optiku a nano-fotoniku používané v budoucích kapitolách. Následně je popsána interference vln a jejich praktické použití pro prolomení difrakčního limitu pomocí holografie plazmonů. Prvním experimentální sestavou je Machův-Zehnderův holografický mikroskop. Je popsán způsob, jak ho sestavit z běžně dostupných součástek a jak je navržen ovládací software k jeho používání. Následně jsou popsány výsledky naměřené pomocí tohoto zařízení. V poslední kapitole se zaměříme na sestavení holografického SNOM přístroje pro studování plazmonů a jejich interference. Nejdříve popíšeme základní princip a navržení softwaru pro automatizaci měření, pro zrychlení vědeckých postupů. Nakonec předložíme výsledky měření mikroskopu.
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Nanophotonics
Dvořák, Petr ; Rezek, Bohuslav (referee) ; PhD, Jakub Dostálek, (referee) ; Šikola, Tomáš (advisor)
This thesis deals with an experimental research of the surface plasmon polaritons (SPPs) using Scanning Near-field Optical Microscopy (SNOM). The first chapter provides theoretical background and a description of most of the physical phenomena and relevant dependencies studied in this work. These dependencies include the dependence of the resulting SPP image on homogeneity, polarization, wavelength and phase of the illumination, on the geometry of the interference structures and on the tilt of the sample with respect to the illumination. Further, this work presents a new experimental method which, using the numerical simulations or SNOM measurements, allows to estimate the sensitivity of SNOM probe to detect the individual electric intensity components of the near-field. At the end of the thesis, the work presents a new microscopic technique which enables a 3D quantitative imaging of phase distribution above the plasmonic metasurfaces.
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