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Rezistence blýskáčka řepkového (Meligethes aeneus, Fabr. 1775) k pesticidům na vybraných lokalitách jižní Moravy
Kubátová, Kateřina
My thesis is devoted to the resistance of pollen beetle (Meligethes aeneus, son. Brassicogethes aeneus Fabricius, 1775) to pesticides in selected localities in southern Moravia. Collecting adults was performed in the spring of 2013 by tapped into polyethylene bags. Adults are collected from the stands of oilseed rape, mustard and poppy. Tests were performed on the basis of the methodology of Insecticide Resistance Action Committee number 011, 021, 025. In total, we tested the pollen population from 18 locations. For testing, we used the following chemical substances: Biscaya 240 OD, cypermethrin, lambda-cyhalothrin, tau-fluvalinate, etofenprox, chlorpyrifos(ethyl). We evaluated 100% of the registered dose of pesticides after 24 hours. From the results of the individual populations, we found large differences in resistance to chemicals.
Rezistence blýskáčka řepkového (Meligethes aeneus, Fabr. 1775) k insekticidům
Jetmarová, Zuzana
The aim of this thesis was to find the current occurrence of resistant sub-population of pollen beetle (Meligethes aeneus Fabricius, 1775) to the selected species active ingredients and the preparation. Adults of pollen beetles were collected from commonly used fields of oilseed rape plants at the time of the first process of flowering until the end of the entire flowering process. The sample collection was carried out during years 2012 and 2013 in twenty locations in the Czech Republic. The Levels of resistance were assessed in a laboratory test called Adult-vial- Test according to official methods used by the International Resistance Action Committee (IRAC). The first part of this thesis describes the biology of pollen beetles. It also describes other pests found on the oilseed rape plant. It further explains the basic concepts and summarizes the occurrence of resistant sub-populations of Pollen beetle in the Czech Republic and other European countries. The second part of this thesis constitutes the practical aspect of the research where there is testing of the resistance of sub-populations of pollen beetles on used active ingredients and the preparation of Biscaya 240 OD.
Rezistence blýskáčka řepkového (Brassicogethes aeneus Fabricius, 1775) k různým druhům insekticidů
Tóth, Pavel
The aims of my PhD thesis are to find out occurence of resistance population of pollen beetle, degree of resistance in population and to suggest possible change of the antiresistant strategy. At the same time it was investigated identification of pollen beetle species (Brassicogethes spp., Meligethes spp. and others) and their dominance in oilseed rape. Populations were collected from commonly used fields of oilseed rape, mustard and poppy in the region of central and southern Moravia and Bohemian-Moravian Highlands in 2009, 2010, 2011, 2012. The individuals were exposed active ingredients lambda-cyhalothrin, ethofenprox, cypermethrin, tau-fluvalinate, chlorpyrifos (ethyl) and the preparation Biscaya 240 OD according to methodology of Insecticide Resistancee Action Committee (IRAC) number 011, 021, 025. Decreased effectivenes active ingredients from group pyrethroids a neonicotinoids among individual year were found. There are resistance populations. The Cross Resistance were discovered between active ingredient lambda-cyhalothrin and ethofenprox. Active ingredient chlorpyrifos (ethyl) were the most effective of all active ingredients which were tested. Pollen beetle (Brassicogethes aeneus) was the most frequent species in all compared samples (2009 -- 2011). From the accompanying species B. subaeneus and B. viridescens were markedly more frequent in this study. To find out differences among the individual pollen beetle species during the laboratory tests of their susceptibility to insecticides is very important. It shouldn't used or combined active ingredient lambda-cyhalothrin and ethofenprox and another active ingredient should changed intensively.
Methodology for evaluation of resistance of pollen beetle to insecticides
Stará, Jitka ; Falta, Vladan ; Kocourek, František
The methodology of evaluation of pollen beetle resistance to insecticides includes approaches that enable easy and reliably to detect resistance to pyrethroids and organophosphates. The methodology includes also information about mechanisms of resistance of insects to insectidies and methods, how to evaluate the resistance. To prevent or slow-down development of resistance in pollen beetle, antiresistant strategies are proposed.
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