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Study of plasma protein binding of radiopharmaceuticals
Hafinec, Václav Matyáš ; Lázníček, Milan (advisor) ; Ramos Mandíková, Jana (referee)
Study of Plasma Protein Binding of Radiopharmaceuticals Summary The purpose of this work is the study of binding of substances (177 Lu-DOTA- [Lys3]bombesin, 177 Lu-NOTA-[Lys3]bombesin, 177 Lu-PCTA-[Lys3]bombesin, and 177 Lu- DOTA-MG47) to plasma proteins by equilibrum dialysis in 37řC, particularly using plasma samples of beef, rabbit, rat and human. Within this group, these substances were compared interspecifically. The substances 177 Lu-DOTA-[Lys3]bombesin, 177 Lu-NOTA-[Lys3]bombesin, 177 Lu- PCTA-[Lys3]bombesin, and 177 Lu-DOTA-MG47 are the newly developed receptor- specific radiolabeled peptides. For all the newly collected data, the interspecific comparison and subsequent statistical evaluation was performed. The indicated bombesin derivates were compared and statistically analyzed even between themselves. During the interspecies comparisons and the determination of the statistical significance of the data, there were found statistically significant and statistically highly significant differences between some of the examined samples. A highly significant difference was found during comparing with samples of 177 Lu-NOTA- [Lys3]bombesin and statistical evaluation, there was found a statistically highly significant difference. Despite the differences found, it is clear that the plasma binding concerning...
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The role of the radiologist assistant in radionuclide brain examination
Horčičková, Zuzana ; Vlček, Petr (referee) ; Špalková, Ingrid (advisor)
Bachelor's thesis "The Role of the radiotherapist in radionuclide brain examinations" is engaged Nuclear medicine imaging methods in the examination of the brain and the role of the radiotherapist at radioisotope nuclear examination in the brain. The work is divided into nine parts. In the first and second part describes anatomy and physiology of the nervous system. The third part is devoted to regional brain blood perfusion - an examination of brain perfusion and assessment of cerebrovascular reserve capacity, which is described preparation and advance in examination, used radiofarmaceutical, evaluation of SPECT examination and contraindication. For testing cerebrovascular reserve capacity are described options burdens. Furthermore, in this chapter refers to the differential diagnostics of dementia, the detection of epileptic focus, the diagnosis of brain death and diagnostics of brain trauma. The fourth part is direct on receptor diagnostics. With respect to commercially supplied by diagnostic ligand is the most common examination dopamine system. The fifth part deals with 18F-FDG - PET examination of the brain. Distribution and intensity of glucose metabolism in the brain can be visualized. The sixth part deals with the diagnosis of brain tumors. In this chapter described the examination using of SPECT...
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Study of interspecies differences in plasma protein binding of 18F-labeled radiopharmaceuticals
Nováková, Dana ; Lázníček, Milan (advisor) ; Bárta, Pavel (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Candidate: Mgr. Dana Nováková Consultant: Prof. PharmDr. Ing. Milan Lázníček, CSc. Title of Thesis: Study of interspecies differences in plasma protein binding of 18 F-labeled radiopharmaceuticals In this thesis, the binding ability of three radiopharmaceuticals containing radionuclide 18 F to the plasma proteins was examined in bovine, pig, human and rat plasma, and the interspecies comparison was performed. Examined radiopharmaceuticals included 18 F-FDG, 18 F-fluorocholine and 18 F-thymidine. The two main methods used are equilibrium dialysis and ultrafiltration. These methods are based on separation of free ligand from the linked component. During the separation, semi-permeable membrane and special filters (AMICON and VIVASPIN) were used for equilibrium dialysis and ultrafiltration, respectively. Free fraction of drugs is considered to be an important parameter for the availability of the drug distribution into the tissues and cells, interaction with receptors, excretion and metabolism. All experiments were carried out at the physiological temperature of 37řC. Obtained results indicate low plasma protein binding of the three radiopharmaceuticals in all studied species, which suggests...
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A patient after a diagnostic application of radiopharmaceuticals as a source of radiation - mutual radiation strain of the personnel and patients
MARKOVÁ, Iveta
ABSTRACT A patient after a diagnostic application of radiopharmaceuticals as a source of radiation - mutual radiation strain of the personnel and patients My work focuses on the radiation protection on account of the potential influence of ionizing radiation with its negative effects on a human organism. In my work I concentrated on observing the obtained personal equivalent doses of the ionizing radiation in the Department of Nuclear Medicine in the Hospital of České Budějovice, plc. I also focused on the measurement and assessment of the dose rate obtained by the employees of the Department of Nuclear Medicine in the Hospital of České Budějovice, plc. from treated patients with regard to the distance and type of the applied radiopharmaceutical. The methods chosen for the actual measuring, gathering, recording and assessment of the data are described in a great detail and presented in the methodology of my work. The data obtained from the personal TL dosimeters is registered and filed in the Department of Nuclear Medicine in the Hospital of České Budějovice, plc. on a long term basis. Therefore it enabled me to analyze the equivalent dose of four nurses during the last five years. The results were compared with the figures determined in the regulation 307/2002 of the Code, as amended by regulation 499/2005 of the Code. The results are then clearly presented in tables and graphs. The collected data has confirmed that the values of equivalent doses obtained from the personal TL dosimeters of four nurses working in the Department of Nuclear Medicine in the Hospital of České Budějovice, plc. do not exceed figures determined by the regulation 307/2002 of the Code, as amended by regulation 499/2005 of the Code. The measured dose rates decrease with the distance and are proportional to the activity and type of radiopharmaceutical.
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Effect of New Positron Radiopharmaceutical Shielding Equipment on Radiation Load Reduction of PET Centre Personnel.
MAŇASOVÁ, Olga
The bachelor thesis focuses on radiation protection of nuclear medicine personnel while using positron radiopharmaceuticals. The thesis is aimed at evaluating efficient and equivalent doses of PET centre personnel at Na Homolce Hospital where new shielding equipment with an activity dosing device was installed in 2006. Positron radiopharmaceuticals are characterized by emission of 2 annihilation gamma photons of high energy (511 keV) that are created upon interaction of a positron with an electron. Due to the high energies, radiological personnel receives higher radiation loads than upon application of common, particularly technecium-based radiopharmaceuticals used in nuclear medicine. The methodology consists in comparing efficient and equivalent doses before and after installation of the new shielding equipment. On base of results we can state that the doses to personnel were significantly reduced after installation of new shielding equipment. The doses were reduced by half during three years. New shielding is relevant from optimalization of radiation protection perspective. Statistical processing and graphic illustrations form part of the bachelor thesis.
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