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National Repository of Grey Literature 12 records found  previous11 - 12  jump to record: Search took 0.01 seconds. 
Selected microbial processes in a bioreactor
 Klinková, Lucie ; doc.RNDr.Petr Zbořil, CSc. (referee) ; doc.Ing.Martin Mandl, CSc. (advisor)
This thesis focuses on the study of the influence of selected parameters on the course of microbial cultivation and evaluation of bioprocess. It is divided into two parts. The first part deals with the production of mutant forms of the protein cryptogein yeast Pichia pastoris. The theoretical part summarizes the findings of the yeast P. pastoris and its expression. It also deals with cryptogein that induces defense reactions in plants. In the experimental part was produced mutant cryptogein X24, in which the concentration of each fraction and the ability to transfer sterols. The second part of this thesis is focused on aerobic and anaerobic oxidation of elemental sulfur by Acidithiobacillus ferrooxidans. In the theoretical section, our knowledge on A. ferrooxidans, its metabolism and the importance of ATP in cell metabolism was summarized. In the experimental part, the above bioprocess was monitored using pH, biomass concentration, the rate of oxidation of elemental sulfur the cellular ATP content.
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Expression and characterization of Phr2 protein of Candida albicans
 Vlčková, Zuzana ; Ing.Zuzana Firáková (referee) ; Kováčová, Kristína (advisor)
Transglycosylases have an import function in yeast the cell wall. These enzymes cleave a molecule of donor, which is followed by transferring a newly created reducing end to a non-reducing end of a molecule of an acceptor. This process enables a growth, expansion and shaping of the yeast cell walls during their life. The aim of this Bachelor Thesis is to characterise the role of Phr2 recombinant protein of Candida albicans in a transglycosylase activity. Using fluorescent paper method, optimal pH and optimal temperature of transglycosylase activity of Phr2 protein were found. The same method was used to establish the acceptor and donor specificity of Phr2p was performed as another method for substrate specificity measurement. High donor and acceptor specificity of Phr2 protein to -1,3-glucan was confirmed. HPLC products of transglycosylase reaction catalysed by Phr2p with laminarin as a donor and L5-SR as an acceptor containing a fluorescent marker were detected by TLC and MALDI-TOF.
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National Repository of Grey Literature : 12 records found   previous11 - 12  jump to record:
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