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Vliv suroviny a technologického postupu při výrobě rybích klobás
Klvaňová, Petra
This bachelors thesis follows up the effects of raw material and technological procedure in production of fish sausages. The sausages for the experiment were made of refrigerated or frozen muscles of the Carp (Cyprinus carpio). Then an organoleptic analysis was done, we evaluated these descriptors: overall look, the surface of the intestine, texture, appearence on the cut, scent, taste, salinity of the product and overall impression. Samples were put forward cooled (4 °C) and warmed (90 °C/15 minutes). The organoleptic analysis determined these results. The fish sausages made of refrigerated material and evaluated as cold had these most rated descriptors; scent and overal impression. On the other hand the fish sausages made of frozen material and evaluated cold, did not get positive score in any of descriptors. By analysing the fish sausages made of frozen material and evaluated cold, were the most rated decriptors; texture, appearance on the cut and taste. However, the fish sausages made of frozen material and evaluated warmed, were the most rated decriptors; overal look, the surface of the intestine and salitiny of the product. Based on the results we can say that the most suitable material for producing the fish sausages are frozen muscles. The technological procedure did not have any influence on the final product.
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Manipulace zárodečných buněk jako nástroj pro management a produkci izogenních linií u ryb
FRANĚK, Roman
Isogenic lines in fish represent a fundamental approach to control the genetic background of experimental animals. All individuals from a given isogenic line share the same genotype. So far, isogenic fish lines have been produced only by repeated uniparental inheritance - androgenesis and gynogenesis. Homozygous progeny is produced in the first generation of uniparental inheritance, and each homozygous individual produces a different isogenic line after second generation of uniparental inheritance. Despite optimized procedures for inducing uniparental inheritance, isogenic lines have been successfully produced in only a few species of fish. Doubled haploids after first uniparental inheritance have affected fitness as well as reproductive performance. Long-term maintenance is considerably problematic even when isogenic line is established already, due to low viability and poor reproductive characteristics. The situation is further complicated by the fact that isogenic lines are usually naturally monosex, thus uniparental inheritance must be re-used for further reproduction, or sex reversal needs to be applied in part of isogenic line. Several types of germ cell manipulation were performed in presented thesis. Protocols for cryopreservation of spermatogonia and oogonia have been developed and optimized to maximize post-thaw viability. The physiological activity of cryopreserved cells was confirmed by transplantation into a surrogate host. Cryopreserved and subsequently transplanted cells retained colonization activity comparable to non-frozen control germ cells. More importantly, male germ cells were able to transdifferentiate from oogonia. The success of transplantation was confirmed by detection of expression of genes associated with gametogenesis in carp by RT-PCR. In the next study, the results of cryopreservation experiments were followed, where sterile goldfish was identified as a suitable host for homozygous carp cells. Germ cells obtained from several homozygous individuals were individually transplanted into sterile goldfish. This procedure has a potential to increase the chance of producing a viable gamete for isogenic line production. Germ cells from homozygotes with affected gametogenesis can be transferred to fully viable recipients, thereby increasing the efficiency of isogenic line production overall. In addition, the use of a goldfish as a surrogate parent will ensure that part of the germline chimeras will be male and female, thus isogenic gametes of both sexes can be obtained and no further intervention for further reproduction of the isogenic line. The suitability of triploid zebrafish, which can potentially be used as recipients for cells from homozygotes to produce isogenic lines, has been confirmed for zebrafish. Spermatogonia and oogonia from diploid donors were transplanted into artificially induced triploid larvae. Donor-derived sperm was were obtained upon maturation of triploid recipients. Transplanted oogonia transdifferentiated into spermatogonia and spermatozoa with female sex chromosomes have been produced, which may be of interesting for further studies of sex determination in zebrafish. A new germline transfer technique has been developed using striped embryos. Donor cells were transplanted from the blastula stage to the swim-up larvae. With this approach, undifferentiated primordial germ cells were able to colonize the genital groove and initiate gametogenesis. After reaching sexual maturity, germline chimeras were obtained with gametes and viable progeny. Although the overall efficacy of this method was lower compared to other transplantation methods, this study may be of relevance for germline rescue in poorly viable embryos or lethal mutants.
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Stravitelnost krmiv pro kapra obecného
MAREŠ, Josef
This bachelor thesis evaluates the composition and apparent digestibility of various types of fish feed and various ingredients applicable to the feeding of common carp. This bachelor thesis also evaluates the suitability of individual components based on the digestibility of individual nutrients. The results show that the right combination of fish feed improves efficiency, reduces feed consumption and increases growth. Last but not least, the negative impact of feeding on the quality of the aquatic environment should be reduced. This bachelor thesis summarizes existing digestibility values of individual feed ingredients for common carp and then its own experiment, which was very time- consuming. Some already known values have been confirmed and some new information about the content of individual components of feed and their subsequent use has been found. The results for wheat and maize were compared with results of other authors. Triticale, daphnia and chironomidae larvae were used for the first time, so the values could not be compared, nevertheless it could be used as a basic information for further research in this area. The average content of individual cereal parameters were: 16% protein content and 64% digestibility, 2% fat content and 70% digestibility, 74% BNLV content and 74% digestibility. Fiber content 5% and ash content 2% with very low digestibility. Phosphorus content 0.6% and digestibility 30%. Average content of individual parameters for natural foods was: protein content 55% and digestibility 86%, fat content 5% and digestibility 74%, BNLV 20% and digestibility 41 %, fiber content 7% and digestibility 8%, ash content 14% and digestibility 30%, phosphorus content 1.2% and 74% digestibility. The resulting element was to determine the most suitable feed for pond aquaculture. According to the available results, wheat combined with natural food is the best option. Wheat is very energy rich, but has a low protein content. Conversely, natural food is energy rich in easily usable protein. The optimal ratio of natural food to wheat was 0.25:2. With this ratio we can meet the ideal nutritional requirements of common carp (31% digestible protein, 0.6-0.7 % phosphorus, 3200 kcal?kg-1 digestible energy). With the right feed, we are able to reduce the negative impact on water quality and this is one of the main goals for the future.
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The impact of vertical distribution of zoobenthos in a pond in response to the biomass stocking of carp ponds
VÁLEK, Pavel
The impact of vertical distribution of zoobenthos in a pond in response to the biomass stocking of carp ponds was tested in the Rod, Dobrá Vůle, Pohořelec, Haslauerteich and Neuteich ponds. Except for the Rod pond, various carp biomass was represented in all ponds. Sampling was conducted during the growing season (June and July), and outside the growing season (September). Physico-chemical parameters of water were measured for each sample. All samples were sorted by groups, counted and weighed. The results are presented in graphs. Zoobenthos density of the Dobrá Vůle, Haslauerteich and Neuteich ponds showed a rising tendency towards deeper parts of the pond sediment. The density of these ponds reached the maximum values in depths of 10-14 cm. This tendency was observed in the occurrence of midges in these ponds. The highest density of midges was observed in the Haslauerteich pond (1,687 individuals per square metre). The above-mentioned ponds were stocked by the common carp (K2-3). The largest density of the tubifexes in the Dobrá Vůle, Haslauerteich and Neuteich ponds was observed during the September sampling in the shallow parts of the sediment (0-6 cm). The maximum density was 1,031 individuals per square metre. On the Pohořelec pond, the occurrence of zoobenthos during both samplings was recorded only in the depth of 10-12 cm. Other representatives of zoobenthos were present in the Rod, Haslauerteich and Neuteich ponds. Larvae of Sialis and Chaoboridae were observed as well. The density and biomass was greater in the September sampling. We have noticed a greater settlement in various depths. Their maximum density was in the Haslauerteich pond (218 individuals per square metre).
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The impact of vertical distribution of zoobenthos in a pond in response to the biomass stocking of carp ponds
VÁLEK, Pavel
The impact of vertical distribution of zoobenthos in a pond in response to the biomass stocking of carp ponds was tested in the Rod, Dobrá Vůle, Pohořelec, Haslauerteich and Neuteich ponds. Except for the Rod pond, various carp biomass was represented in all ponds. Sampling was conducted during the growing season (June and July), and outside the growing season (September). Physico-chemical parameters of water were measured for each sample. All samples were sorted by groups, counted and weighed. The results are presented in graphs. Zoobenthos density of the Dobrá Vůle, Haslauerteich and Neuteich ponds showed a rising tendency towards deeper parts of the pond sediment. The density of these ponds reached the maximum values in depths of 10-14 cm. This tendency was observed in the occurrence of midges in these ponds. The highest density of midges was observed in the Haslauerteich pond (1,687 individuals per square metre). The above-mentioned ponds were stocked by the common carp (K2-3). The largest density of the tubifexes in the Dobrá Vůle, Haslauerteich and Neuteich ponds was observed during the September sampling in the shallow parts of the sediment (0-6 cm). The maximum density was 1,031 individuals per square metre. On the Pohořelec pond, the occurrence of zoobenthos during both samplings was recorded only in the depth of 10-12 cm. Other representatives of zoobenthos were present in the Rod, Haslauerteich and Neuteich ponds. Larvae of Sialis and Chaoboridae were observed as well. The density and biomass was greater in the September sampling. We have noticed a greater settlement in various depths. Their maximum density was in the Haslauerteich pond (218 individuals per square metre).
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Kryoprezervace a transplantace spermatogonií kapra obecného
FUČÍKOVÁ, Michaela
Cryopreservation and transplantation of germ cells in fish provides a suitable tool for preserving genetic information. By method of surrogate reproduction, the offspring with characters of the chosen donor can be obtained. In this case of our commercially important species common carp. However, for the successful cryopreservation of the germ cells, a suitable protocol for each species must be established. Several cryoprotectants were tested. The best of them, Me2SO, regarding the viability of spermatogonia, was tested for its different concentrations depending also on the rate of freezing. Further testing, related to the effect of tissue size, incubation time and added sugar, was performed. The result of the assay identified best cryomedium composed of 2.5M dimethylsulfoxide, added sugar of 0.3M glucose, 1.5% BSA and 25nM Hepes dissolved in PBS. The most suitable size of tissue was 100 mg, incubation time was 30 min and coolig rate was -1 ° C/min. This protocol ensures the highest viability rate of cryopreserved spermatogonia of common carp. The second part of the work was to verify the success of the transplantation of cryopreserved and fresh spermatogonia into a suitably chosen recipient, the goldfish, which shares similar reproductive characteristics with carp, but also offers reduction of space requirements or resistance to koi-herpes virus. The transplanted germ cells colonized the germ line and started gametogenesis in 42.5% (cryopreserved spermatogonia) and 52.5% (fresh spermatogonia) goldfish recipients, which demonstrated that the transplantation of cryopreserved spermatogonia of common carp can be successfully achieved.
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The potential of compound feed and cereals as a tool for keeping the production of carp at a good level in relation to the water quality.
MELKA, Václav
The aim of this diploma thesis was to test the production effectiveness of cereals and compound feed and at the same time, find out what influence they have on the water quality when applied in the semi-intensity carp farming. The feeding test was taking place in the period of 112 days in 10 experimental storage ponds. The feed which was tested, was cereals, extruded feed from the Aller Aqua company with 24% share of protein, pelleted feed glycid type feed with the 12,5% share of protein (KP) and pellet feed glycid type compound feed with 12,5% share of protein and reduced share of phosphorus (KPminus). A tested carp population of the same density was left without any additional feeding, to be fed only on the natural nutrition. Every one of these separated carp populations were fed three times a week in the morning and on the same spot of the fish tank. The testing was taking place twice.The measuring of the parametres of the water (temperature, oxygen, pH) took place once a fortnight, taking samples of the water once a month. At the end of the experiment, the level of phosphorus and the observed production parametres were evaluated.The highest production effectiveness was reached in the carps that were fed with cereals (FCR - Food Convertion Ration 2,41 - 0,46, SGR - Specific Growth Rate 0,54 - 0,07 %.d-1). The lowest production effectiveness was achieved with the pelleted feed KPminus (FCR - 3,72 - 0,30, SGR - 0,39 - 0,02 %.d-1). The highest retention of phosphorus (88%) in the fish biomass was reached with cereals. The lowest retention of phosphorus (60%) was reached in the carps fed with the pelleted feed KP. Feeding carps with tested feeds did not have any significant influence on the water quality. From both the economic and the environmental point of view, the best feed for carp pond farming is cereals.
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Využití vybraných komponentů rostlinného původu ve výživě ryb
Zugárková, Iveta
The main aim of this Bachelor´s thesis was to create a literary review on the use of selected plant components in fish nutrition. Part of the review contains data on fish bre-eding and nutrition, nutritional requirements regarding the nutrients and customization of fish food. The second part of the Bachelor´s thesis presents a draft on the methodo-logy of a feeding test. Both scaled and mirror forms of common carp (Cyprinus carpio L.) will be used in this test and fed different crops: feeding and low-phosphate wheat, feeding and pearl barley. The corps will be processed into granule to make the food intake and utilization easier. The test will star in May 2016 and be carried out in the recirculation system of the aquarium room at the Department of fishery and hydrobiolo-gy of the Mendel University in Brno. The aims of the test is to evaluate the production effectiveness of used crops, including their influence on the chemical composition of fish tissues and selected parameters of internal environment of fish bodies. The tested crop species were chosen based on the data on the influence of various crops on the phosphorus level reductions in fishponds. Because the plant food for carp represents the most expensive part of carp breeding, there is ongoing research for finding economically and nutritionally suitable food.
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Vliv vybraných těžkých kovů na fyziologii ryb
Šanda, Radek
The study aims on effects of mercury on haematological and biochemical parameters in blood plasma on common carp (Cyprinus carpio L.). The theoretical part of study is focused on phenomenon of occurrence of mercury and its forms in aquatic environment and fish. Practical portion of study includes the effects of administration to mercury and also the effects of co-administration of sodium chloride. In the first experiment we carried out the exposure of Cyprinus carpio L. by selected mercury concentrations for 5, 10 and 15 days. In the second experiments were carps exposed to 1.5 micro g.l-1 mercury with the addition of various concentrations of sodium chloride (29 mg.l-1, 300 mg.l-1 and 1000 mg.l-1) for 72 h. For the evaluation of haematological and biochemical biomarkers in blood plasma of carps, we exploited the Konelab T 20xt and Easy Lyte machines. The obtained data were statistically processed and evaluated.
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Diet of great cormorant (Phalacrocorax carbo) in conflict localities
Ráslová, Pavlína ; Čech, Martin (advisor) ; Vrána, Pavel (referee)
vi Abstract Continental subspecies of the great cormorant (Phalacrocorax carbo sinensis) is the Czech Republic's most important fish-eating predator. It causes damage to production fish, as well as sport fishermen and even after its removal from the Czech list of endangered species in 2013, it did not disappear from the spotlight of both local fishermen and researchers. This thesis evaluates the qualitative and quantitative composition of the cormorant's diet during winter 2011/2012 at two selected locations: the Žehuň pond (Kolín district) and Berounka river in the natural reserve Zábělá near Pilsen. This work studies the main components of cormorant's diet in the selected locations and evaluates potential fluctuations and changes in its composition during winter. The study also conducted the analysis of the proportion of commercially harvested valuable fish in cormorant's diet and studied its diversity of the species spectrum. The resulting data for the two sites were compared with each other and with relevant literature. Regurgitated pellets analysis, as well as analysis of diagnostic bones and fish remains located at the sleeping roosts (night roosts) were used for the diet analysis. It was found that at both sites roach was the most caught fish, but from the point of view of total biomass, it was...
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