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Important food-borne pathogens in raw cow´s milk
HRUBEŠOVÁ, Kristýna
This work talks about the problems of food-borne pathogens in raw milk, with a special focus on Campylobacter jejuni and Staphylococcus aureus including its negative influence on health of the consumers. The topic of consumption of raw milk was lately a much discussed question and is further described in the text. With consideration to possible occurrence of food-borne pathogens, it is important to thermally treat raw milk before consumption. Despite this fact there is still a large number of consumers who don?t respect this technical procedure and they threaten their own health.
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Health problems in Ara chloroptera and Ara macao breeding (review)
JANDOVÁ, Ivana
Red and green Macaw (Ara chloroptera) and Scarlet Macaw (Ara macao) belong to the most bred big parrots in our country. They are resistant species, however, in breeding they can suffer from different illnesses. The aim of this work was to deal with the existing knowledge of their veterinary problems. The work is divided into several main parts according to the qualities of illnesses. There are infectious and invasive diseases, genetic disorders, tumoral diseases or veterinary problems caused by environmental impact. In the last but one part different injuries of these birds are mentioned and the last part contains health problems of digestion, respiration or psyche. Finally, basic aspects of the health problems in the breeding of these species are analysed.
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Poultry cryptosporidiosis
KURAL, Vladimír
A total of 270 samples of domestic hen (Gallus gallus f. domestica) from 20 farms were collected during two consecutive years (from 2011 to 2012). Microscopical examination of aniline-carbol-methyl violet stained fecal smears revealed 5 positive samples originating from one farm. DNA was extracted from Cryptosporidium positive samples and all microscopically negative samples. Nested PCR was performed to amplify the partial SSU rRNA gene of Cryptosporidium. The sequence analyses of PCR-positive specimens identified 8 samples as a novel genotype, working titled Cryptosporidium hen genotype. The sequences identified as hen genotype matched most closely with Cryptosporidium bovis which was originally reported from cattle in the most cases.
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Methodics of molecular detection of Barley yellow dwarf virus in vectors by RT-PCR
Jarošová, Jana ; Jaňourová, Blanka ; Kumar, Jiban
Barley yellow dwarf is one of the most important viral diseases of cereals in the world. It can cause significant yield losses in most cereal species, including wheat, barley, rice, corn, oat and rye. In this methodology we provide assays for reverse transcription - polymerase chain reaction (RT-PCR) for sensitive detection of barley yellow dwarf virus directly in the vectors, in aphids. This way it is possible to evaluate infectivity of vectors and from that conclude subsequent measures. The methodology is meant to serve the employees of State Phytosanitary Administration.
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