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There is something in the air: Diversity of lichen symbionts in the aeroplankton
Veselá, Veronika ; Škaloud, Pavel (advisor) ; Vančurová, Lucie (referee)
Lichens that reproduce primarily sexually rely on the presence of their symbionts in the environment. A new lichen cannot develop without direct contact between the filaments of the germinating spore and a compatible symbiotic alga. However, the results of earlier studies do not provide solid evidence for photobiont presence on lichen-colonized substrates. The objective of this thesis was to assess various environmental types (air, bark, soil, stone, and artificial substrates) as potential sources of symbiotic algae. To achieve in-depth understanding of the diversity of green algae at a long-studied Central Bohemian study-site, Illumina MiSeq metabarcoding of the ITS2 rDNA region was combined with traditional sequencing of lichen algal ITS rDNA and morphological observations. The methods used revealed 283 species-level lineages and 1313 OTUs, many of which belonged to unknown species or higher taxonomic categories. All photobiont species associating solely with sexual lichens at the site could also be found in the vicinity of lichen thalli. As a result, both the substrates and the air, where these symbiotic algae occurred as well, may act as photobiont sources. In contrast, many symbionts that associate only with asexual lichen species, were never found at the site outside of lichen thalli....
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Charakterisation of fungal aerosol in size segregated aerosol
Shamratova, Kseniia ; Hovorka, Jan (advisor) ; Kozáková, Jana (referee)
This bachelor thesis deals with the issue of bioaerosol, properties of airborne fungal spores, describes their significance, concentration, methods of spreading and influence on human health. This work provides information about sampling and detection methods of bioaeerosol. Was proved the suitability of using of the first step of the high volume cascade impactor (Hi-Vol) BGI-900 to collect biaerosol samples for subsequent scanning electron microscope (SEM) analysis. Using the optimized method, bioaerosol samples were isolated from the polyurethane foam, which is the impact substrate of the impactor. Bioaerosol samples were taken from 3 locations in the Czech Republic: Prague, Březno, Láz. During the SEM analysis of 15 bioaerosol samples, 605 SEM images were taken, on 340 of them were identified fungal spores. The smallest number of spores were found in Prague. Approximately the same amount of fungal spores was found in Březno and Láz. Fungal spores were identified into 9 genus, 1 family, and 1 species. The percentage of deformed spores in Prague was 48%, in Březno 23%, in Láz 25%. The greatest variability of spores was at the Březno and Láz localities. The most common fungal genus was the Cladosporium genus. No positive or negative correlation was observed between the number of fungi and the...
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Pollen charakterisation in size segregated aerosol
Kolpakova, Anna ; Hovorka, Jan (advisor) ; Zíková, Naděžda (referee)
This bachelor thesis, in literature review, deals with bioaerosol issue, describes meaning of pollen, ways of pollen spreading and its influence on human health, summarize methods of pollen sampling from the air and common ways of pollen grains identification. In the experimental part, the use of the first stage of a High Volumetric Cascade Impactor (Hi-Vol) BGI-900 for bioaerosol sampling and subsequent SEM analysis was proved. Method of pollen separation from the impaction substrate, polyurethane foam, into homogeneous deposit on Nylon filter was optimized. Representative portion of the deposit was analyzed by SEM. There were taken 485 SEM pictures from 12 samples in 3 localities in the ČR. Pollen grains were identified in 295 SEM and determined into 9 genus and 4 families and grain deformities were quantified to be 24, 18, 50% for Prague, Brezno and Láz localities. Number of pollen increased with total aerosol mass in Prague locality only. Thre aleo identified insect secrction products borochomomes in the samples. Key words: pollen, pollen grain, bioaerosol, cascade impactor, SEM.
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Bioaerosols
Hladíková, Dita ; Hovorka, Jan (referee) ; Hůnová, Iva (advisor)
Bioaerosols and aerobiology are similar terms associated with the study of chemical, physical and biological fields. Bioaerosols in the air is a part of the aerosol containing particles of biological origin, such as viruses, bacteria, fungal spores, house dust mites and pollen. These particles can affect health of living organisms in terms of allergies, toxicity, carcinogenicity or infection and ultimately affect the planet's climate system because of acting as condensation nuclei for the formation of precipitation. The most studied biological effects of the above-mentioned on human health are reported respiratory symptoms and pulmonary diseases. This is due to almost continuous human exposure to these particles, both in the domestic environment, which includes mainly house dust mites, animal allergens, molds and bacteria, and outdoors, where a person is exposed to pollen and outdoor molds. In the case of interference with the climate system of the Earth there is a direct or indirect influence. Indirect effects include the creation of clouds and precipitation, which has the effect on the hydrological cycle of the planet. Scattering and absorption of solar radiation is considered a direct effect on climate change. Foreign literature was a crucial source of information on this topic.
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