"CECE 2012. International Interdisciplinary Meeting on Bioanalysis /9./" - Výsledky hledání - Digitální repozitář

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	Influence of the O-phosphorylation of serine, threonine and tyrosine in proteins on the amidic N-15 chemical shielding anisotropy tensors Emmer, J. ; Vavrinská, A. ; Sychrovský, Vladimír ; Benda, Ladislav ; Kříž, Z. ; Koča, J. ; Boelens, R. ; Sklenář, V. ; Trantírek, L. Density functional theory was employed to study the influence of O-phosphorylation of serine, threonine, and tyrosine on the amidic N-15 chemical shielding anisotropy (CSA) tensor in the context of the complex chemical environments of protein structures. Úplný záznam
	Comparison of concentration of ions in PM1 aerosol sampled on nitrate cellulose and teflon filters Kořínková, Alena ; Mikuška, Pavel ; Večeřa, Zbyněk ; Křůmal, Kamil Concentration of ions in PM1 aerosol sampled on nitrate cellulose and teflon filters were studied in this work. Úplný záznam
	Chemical composition and volatility of essential oils investigated in frame of study: Saving of culture heritage on paper Kubátková, Nela ; Křůmal, Kamil ; Večeřa, Zbyněk ; Mikuška, Pavel Chemical composition of essential oils and concentrations of individual components in gas phase were presented in this contribution. Úplný záznam
	Caspase 3 chemiluminiscence activity determination in apoptotic cells and design of Caspase 3 sensor Lišková, Marcela ; Klepárník, Karel ; Pazdera, P. ; Foret, František Apoptosis, or programmed cell death, is an essential physiological process that plays a critical role in development of tumor or autoimmune disorders. In some instances knowledges of this pathway can be used for inhibit or prevent cancer development and other diseases. One of the earliest and most consistent observed features of apoptosis is the induction of a series of cytosolic proteases – caspases. Úplný záznam
	Sensor based on Förster resonance energy transfer with quantum dot Lišková, Marcela ; Datinská, Vladimíra ; Klepárník, Karel ; Foret, František Förster resonance energy transfers (FRET) are based on the nonradiative transfer of energy between a donor and acceptor dye in the distance 1-10 nm. With respect to this, quantum dots (QDs) with a high absorbance can serve as suitable donors of energy in FRET. Here, we present architectures of two sensors based on QD conjugates useful in imaging and diagnostics of cancer. One sensor, based on an oligonucleotide conjugate, can be used for the detection of complementary oligonucletide chains. The other was designed for the detection of enzymes inside cells. Úplný záznam
	Behavior of sheathless and electrodeless electrospray electrophoresis during inorganic ion separation Tarantová, Anna ; Foret, František This work is aimed at optimization of a separation process in a thin capillary for future application in mass spectrometry (CE-MS). We have used an experimental design where the voltage only on the capillary entrance was applied. The opposite end was sharpened into an electrospray tip and situated opposite to the grounded vacuum inlet electrode. This system is called a sheathless and electrodeless interface. Úplný záznam
	A simple device for quantitation of Caspase 3 in individual apoptotic embryonic cells Hegrová, Jitka ; Klepárník, Karel ; Přikryl, Jan ; Lišková, Marcela ; Matalová, Eva ; Foret, František A newly developed system for the determination of Caspase 3 activity in individual apoptotic embryonic cells is described. Caspase 3, a cysteine-aspartic acid protease activated in the apoptotic pathway, plays an essential role in the programmed cell death. Moreover, failure of apoptosis is one of the main contributions to tumor development. Úplný záznam
	A simple sample pretreatment device with supported liquid membrane for direct injection of untreated body fluids and in-line coupling to a commercial capillary electrophoresis instrument Pantůčková, Pavla ; Kubáň, Pavel ; Boček, Petr A simple sample pretreatment device employing extractions across supported liquid membranes (SLMs) was designed for in-line coupling to a commercial CE instrument. The device can be easily assembled/disassembled and SLMs can be replaced after each extraction thus minimizing sample carry-over, avoiding tedious SLM regeneration and reducing total pretreatment time and costs. Úplný záznam
	Saccharides in PM2.5 aerosols in Brno Křůmal, Kamil ; Kubátková, Nela ; Mikuška, Pavel ; Večeřa, Zbyněk Contribution summarizes the concentrations of saccharides (monosaccharides, disaccharides and sugar alcohols) in PM2.5 aerosols in urban area during different seasons (winter, spring,summer and autumn 2010 and winter 2011). We optimized conditions for the extractions of saccharides from quartz filters. Úplný záznam
	Characterization of atmospheric particulate matter by diffusive gradient in thin film (DGT) technique Gregušová, Michaela ; Dočekal, Bohumil In this study, characteristics of heavy metal contamination in road dust collected from urban area with heavy traffic in Brno, Czech Republic was investigated. The DGT probes were exposed in aqueous suspensions of particulate matter for different time periods to monitor responses of the sample matter. The road dust and DGT sorption gels were decomposed by microwave assisted extraction and analyzed by inductively coupled plasma mass spectrometry for estimation of trace metals contents. The results revealed that the traffic appears to be responsible for the high levels of Cu, Pb, Ni and Sb and also that DGT technique, as alternative to conventional extraction procedures is a useful tool for characterization of metals mobility. Úplný záznam

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