Národní úložiště šedé literatury Nalezeno 2 záznamů.  Hledání trvalo 0.00 vteřin. 
A NOVEL BIOAUGMENTATION APPROACH IN BIODEGRADATION OF ORGANIC POLLUTANTS IN CONTAMINATED SOIL
Lhotský, O. ; Filipová, Alena ; Innemanová, P. ; Velebová, R. ; Cajthaml, Tomáš
Polycyclic aromatic hydrocarbons (PAHs) belong among organic compounds that accumulate in the environment mainly resulting from human activities. Because of their potentially negative effects on human health, methods of their removal from the environment are intensively studied. In this work, a biodegradation method of bioaugmentation based on sequential stepwise mixing of inoculated soil with non inoculated soil was used. The subject of this work was an application of a bacterial consortium on a real contaminated (7399 mg.kg-1 of PAHs) soil. 8 bacterial strains isolated from other contaminated sites were inoculated. DNA was isolated from the samples during the inoculation process and the degradation of PAHs was monitored after 4 months.
TEMPERATURE INFLUENCE ON DESORPTION AND DETERMINATION OF BIOACCESSIBLE PAHS
Čvančarová, Monika ; Pokorná, Lenka ; Velebová, R. ; Najmanová, P. ; Cajthaml, Tomáš
Hydrophobic organic pollutants tend to adsorb onto soil particles which can cause a significant decrease in their bioaccessible fractions. Definition of the bioaccessibility is currently not unified; however it represents a part of total PAHs amount which is potentially available for biological processes. It is an important parameter which can be used for prediction of microbial degradation and environmental risk evaluation. This paper studies desorption behavior of PAHs at 20 and 60˚C. The desorption of PAHs from long-term contaminated soils was evaluated using resin Amberlite XAD- 2. A two-phase desorption model was used to calculate the desorption kinetic curves and F fractions which represent bioaccessible amount of PAHs. The results showed that the desorption of PAHs was significantly influenced by temperature. The greatest differences in the desorption behavior were observed at 5–6 ring PAHs. We compared the F fractions and the rate constants of individual PAHs which were determined at 20 and 60˚C. The results were also compared with sequential supercritical fluid extraction which has been already used for prediction of microbial degradation.

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