Národní úložiště šedé literatury Nalezeno 1 záznamů.  Hledání trvalo 0.00 vteřin. 
Plasma-membrane alkali-metal-cation transporters involved in salt tolerance of pathogenic Candida species
Krauke, Yannick ; Sychrová, Hana (vedoucí práce) ; Malcová, Ivana (oponent) ; Heidingsfeld, Olga (oponent)
of Ph.D. Thesis Conclusions All the aims of the thesis were achieved. The tolerance to alkali metal cations of four pathogenic Candida species was studied in detail and revealed differences among the yeasts. These differences in sall tolerance remained the same under various growth conditions. For the first time, the internal sodium and potassium concentrations of several Candida species were estimated under high salt-stress grolvth. The internal K./Na* ratio was not in relation with the salt tolerance revealing different adaptation mechanisms to salt stress in Candida species. A first study on combinatory use of fluconazole and NaCl revealed severe synergistic effects of both compounds, leading to grovvth inhibition and increased internal Na* concentrationsi n C. albicans.T he molecularb asis of this synergismr emains to be established. Ihe C. dubliniensis, C. glabrata and C. parapsilosis Cnhl NalH- antiporters were cloned and functionally characterized upon heterologous expression in S. cerevlslae to understand the mechanisms involved in the different salttolerances of Candida species. The three antiporters differed in their activity for alkali metal cations, which roughly correlated with the observed differences in salt tolerance among the species. Additionally, during the characterization of...

Chcete být upozorněni, pokud se objeví nové záznamy odpovídající tomuto dotazu?
Přihlásit se k odběru RSS.