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Use of microcalorimetric techniques in the study of stress adaptation of microorganisms
 Korfantová, Stanislava ; Slaninová, Eva (oponent) ; Krouská, Jitka (vedoucí práce)
This bachelor thesis uses microcalorimetric techniques to study stress adaptation of wild strain of H. halophila and H. halophila strain adapted to lower salinity of medium in the environment of levulinic acid, which occurs naturally in hydrolysates of lignocellulosic materials. These can serve as inexpensive substrates on which H. halophila is capable of producing polyhydroxybutyrate. Microcalorimetric measurements showed that the wild-type H. halophila strain could adapt to levulinic acid to a concentration of 5 g/l. The adapted strain, which was assumed to have poorer stress management based on lower PHB yields, could adapt to all observed concentrations. The results were compared with a conventional method that confirmed the results of microcalorimetry. Microcalorimetry has been evaluated as a universal and susceptible method that is suitable for studying the metabolic activity of microorganisms. To better interpret the obtained microcalorimetric results, it is necessary to supplement the technique with information from other analytical techniques. The bachelor thesis uses spectrophotometric and gravimetric determination and gas chromatography with a flame ionization detector to characterize the wild and adapted strain before monitoring the adaptation to levulinic acid. The techniques offered enriching information to supplement the microcalorimetry outputs.
Úplný záznam
Use of microcalorimetric techniques in the study of stress adaptation of microorganisms
 Korfantová, Stanislava ; Slaninová, Eva (oponent) ; Krouská, Jitka (vedoucí práce)
This bachelor thesis uses microcalorimetric techniques to study stress adaptation of wild strain of H. halophila and H. halophila strain adapted to lower salinity of medium in the environment of levulinic acid, which occurs naturally in hydrolysates of lignocellulosic materials. These can serve as inexpensive substrates on which H. halophila is capable of producing polyhydroxybutyrate. Microcalorimetric measurements showed that the wild-type H. halophila strain could adapt to levulinic acid to a concentration of 5 g/l. The adapted strain, which was assumed to have poorer stress management based on lower PHB yields, could adapt to all observed concentrations. The results were compared with a conventional method that confirmed the results of microcalorimetry. Microcalorimetry has been evaluated as a universal and susceptible method that is suitable for studying the metabolic activity of microorganisms. To better interpret the obtained microcalorimetric results, it is necessary to supplement the technique with information from other analytical techniques. The bachelor thesis uses spectrophotometric and gravimetric determination and gas chromatography with a flame ionization detector to characterize the wild and adapted strain before monitoring the adaptation to levulinic acid. The techniques offered enriching information to supplement the microcalorimetry outputs.
Úplný záznam

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