|
|
Bioceramic materials bioactivity testing via simulated body fluid
Holbusová, Eva ; Novotná, Lenka (oponent) ; Salamon, David (vedoucí práce)
These days, there is a possibility of joint replacement implantation, more common dental implants, or regeneration of bone fractures using bone graft replacements is more accessible than in history. Bioceramic materials are used for these purposes. Bioceramics is a non-toxic ceramic material. It is characterized by its bioactivity and biocompatibility. Bioactivity testing of bioceramics is performed using simulated body fluid (SBF). In addition, the change in the concentration of calcium ions was investigated during the testing. The aim of the work was to determine whether the concentration of calcium ions changes between bioceramics and SBF. Bioceramic samples were prepared by two methods, namely freeze-casting and cold isostatic pressing. What was soaked in c-SBF was prepared according to Kokubo. The analysis of SBF was performed using a colorimetric method with a reagent of Murexide solution and reagent KIT. The concentration of calcium ions in the SBF after soaking samples in a testing cycle 0, 3, 7, 14, and 21 days was measured using a UV/VIS spectrophotometer. In addition, X-ray diffractometry was used to determine the phase composition of the material before soaking in SBF. The change in calcium ion concentration during the test cycle in the bioceramic – SBF system was confirmed with UV/VIS spectrophotometry. The most significant increase in concentration was recorded on the third day of soaking. The assumption of an apatite layer formation on the soaked bioceramic samples was supported by a change in the concentration of calcium ions SBF and the change in weight of the material after soaking. It was found that using indirect colorimetric determination delivered better results when the Murexide indicator was used instead of KIT.
|
|
|
Bioceramic materials bioactivity testing via simulated body fluid
Holbusová, Eva ; Novotná, Lenka (oponent) ; Salamon, David (vedoucí práce)
These days, there is a possibility of joint replacement implantation, more common dental implants, or regeneration of bone fractures using bone graft replacements is more accessible than in history. Bioceramic materials are used for these purposes. Bioceramics is a non-toxic ceramic material. It is characterized by its bioactivity and biocompatibility. Bioactivity testing of bioceramics is performed using simulated body fluid (SBF). In addition, the change in the concentration of calcium ions was investigated during the testing. The aim of the work was to determine whether the concentration of calcium ions changes between bioceramics and SBF. Bioceramic samples were prepared by two methods, namely freeze-casting and cold isostatic pressing. What was soaked in c-SBF was prepared according to Kokubo. The analysis of SBF was performed using a colorimetric method with a reagent of Murexide solution and reagent KIT. The concentration of calcium ions in the SBF after soaking samples in a testing cycle 0, 3, 7, 14, and 21 days was measured using a UV/VIS spectrophotometer. In addition, X-ray diffractometry was used to determine the phase composition of the material before soaking in SBF. The change in calcium ion concentration during the test cycle in the bioceramic – SBF system was confirmed with UV/VIS spectrophotometry. The most significant increase in concentration was recorded on the third day of soaking. The assumption of an apatite layer formation on the soaked bioceramic samples was supported by a change in the concentration of calcium ions SBF and the change in weight of the material after soaking. It was found that using indirect colorimetric determination delivered better results when the Murexide indicator was used instead of KIT.
|
host ::
RSS.