National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Effect of Exercise on Lipid Metabolism in White Adipose Tissue
Gazdová, Tatiana ; Horáková, Olga (advisor) ; Tauchmannová, Kateřina (referee)
Obesity and old age are more prevalent among the current human population than ever before. Increased fat accumulation and hormonal dysregulation are common hallmarks of both these states. It is well reported that both states are associated with impaired lipolysis and glucose homeostasis as a result of disrupted metabolic pathway signalling. Current research offers exercise as a possible remedy, which could improve substrate handling in obesity and old age. Increased energy expenditure, myokine production, and improved insulin sensitivity are all potential benefits of exercise that could mitigate the health risks associated with obesity and ageing. In this work we aim to explore the effect of acute endurance exercise protocol on lipolytic activity of visceral and subcutaneous white adipose tissue depots of lean and young, obese, and aged mice. By the addition of forskolin, isoproterenol, and insulin into the explant media we hope to assess the functionality of β-adrenergic signalling in our subjects. Post exercise lipolytic rate, measured as non-esterified fatty acid release, increased in both depots in all test groups. Glycerol release did not follow the same pattern. Additionally, lipolytic rate, measured as non-esterified fatty acid release, in visceral adipose tissue was higher in samples from...
Regulation of immunometabolism in white adipose tissue
Gazdová, Tatiana ; Horáková, Olga (advisor) ; Beňová, Andrea (referee)
High-fat diet promotes the development of diet-induced obesity, which leads to further complications such as insulin resistance and type II diabetes. The underlying cause for development of obesity associated pathologies is disruption of adipose tissue homeostasis. Excessive lipid accumulation and rapid white adipose tissue expansion stimulate infiltration, proliferation, and activation of immune cells involved in inflammation propagation. Immune cells within white adipose tissue have the ability to modulate adipocyte function as well as whole-body metabolism. These interactions and function modulations are the core topics of immunometabolism, a rapidly developing field of research focused on interpreting how immune system modulates metabolism on cellular as well as systemic level. In obesity, pro-inflammatory immune cells, for example M1 macrophages and neutrophils, outnumber homeostasis-promoting anti-inflammatory immune cells in white adipose tissue and alter the tissue environment. As a result, pro-inflammatory cytokines prevent adipocytes from adequately responding to extracellular stimuli. The resulting interactions between immune cells and adipocytes maintain inflammation and promote ectopic lipid accumulation. Experimental studies suggest that white adipose tissue inflammation can be...

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