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Precision-cut placental slices as a model to study inflammatory response
Bolboli, Bahareh ; Štaud, František (advisor) ; Carazo Fernández, Alejandro (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Bahareh Bolboli Supervisor: Prof. PharmDr. František Štaud, Ph.D. Consultant: Kasin Yadunandam Anandam, Ph.D. Title of the diploma thesis: Precision-cut placental slices as a model to study inflammatory response. Maternal inflammation during pregnancy is a recognized factor linked to an array of complications and potential neurodevelopmental and neuropsychiatric risks for offspring. Placenta isthe crucial interface between maternal and fetal domains. This organ not only shapes fetal development but also possesses the ability to respond to inflammatory stimulations, potentially in a sex-specific manner. Nonetheless, the fundamental immunoregulatory mechanisms orchestrating such responses remain unclear. To bridge this knowledge gap, our study harnessed an innovative approach: the ex vivo precision-cut placental slice model using rat placentas. This experimental design was tailored to probe acute inflammatory responses. Precision-cut placental slices, precisely 200 µm thick, were meticulously generated from both male and female rat placentas. These slices were subjected to varying concentrations of Lipopolysaccharide (LPS) or Polyinosinic: polycytidylic acid (Poly I:C) for discrete periods of 4...

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