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Comparative analysis of the communication strategies in chosen media of candidates for the presidential elections in 2023 in the second round
Suchomel, Jakub ; Zima, Michal (advisor) ; Nečas, Vlastimil (referee)
In this thesis, a comparative analysis of the communication strategies of the presidential candidates in the 2023 elections Ing. Andrej Babiš and arm. gen. v.v. Ing. Petr Pavel, M.A. is carried out. The work starts with a description of the election process. The AMI Digital Index 2022, which is a survey in the field of social networks, is presented. In the next chapter, the theoretical framework of the work is presented, starting with the characteristics of the examinated media. The following is a brief description of the course of presidential elections in the past. Then the process of data collection is described and the method of social network analysis is presented. The following are individual analyses of social networks. First, six analyses of Andrej Babiš's communication are presented, as well as Petr Pavel's. This is followed by a description of the most symbolic themes of the individual campaigns. The next part is devoted to the characteristics of the analysis of pre-election debates, and then the analyses themselves and their comparison are discussed. Before the very conclusion, the candidates' post-election communication processes are briefly described. The last part of the thesis is the conclusion, which contains the results of qualitative comparison and comparison of individual...
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The role of mitochondrial energy metabolism in cell senescence
Zima, Michal ; Trnka, Jan (advisor) ; Pecinová, Alena (referee)
Cellular senescence represents status, when the cells cease to divide and remain in permanent cell cycle arrest. Senescence is considered to be an active response of the cell to various extrinsic and intrinsic types of stress such as certain oncogene activation, exposing to several cytokines or drugs and damaged and/or uncapped telomeres. Senescent cells are characterised by extensive modification of gene expression, flattened and enlargement of cellular body. Hypothetically, altered gene expression may lead also to increase of certain surface proteins expression. Such protein can be L1 cell adhesive molecule (L1CAM), which is expressed heterogeneously within the population. This Thesis describes current knowledge of cellular senescence, the mechanism, which may result in establishment of senescence phenotype, and also the characteristic markers of senescence. Thesis also puts together the heterogeneity of L1CAM expression in A375 senescent cells with oxygen consumption rate and extracellular acidification rate performed by Seahorse XFe24 metabolic analyser. Therefore, ells were sorted according to their levels of expressing L1CAM onto low and high L1CAM expressing subpopulations. Obtained data show potential correlation between the rate of L1CAM expression in A375 cells and the metabolic rate. Key...
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Changes in oxidative phosphorylation during development of cellular senescence
Zima, Michal ; Hodný, Zdeněk (advisor) ; Kašparová, Dita (referee)
Cellular senescence represents a state of permanent cell cycle arrest. It is considered to be an active response of the cell to various extrinsic and intrinsic types of stress, which are damaged and/or uncapped telomeres, activation of certain oncogenes, DNA damage and effects of several cytokines. This thesis describes current mechanisms which may result in establishment of senescence phenotype, putting those facts in association with changes in oxidative phosphorylation. In thesis are also mentioned features of senescence cells and their impact on the neighborhood. Special attention is focused on the role of reactive oxygen species in promotion of cellular senescence, mechanisms of their elevation, the role of NADPH oxidases and the inhibition of mitochondrial oxidative phosphorylation complexes by activity of cytokine signaling pathways STAT3 and TGFbeta. Key words: cellular senescence, reactive oxygen species, cytokines, mitochondria, oxidative phosphorylation chain, NADPH oxidases, Signal Transducer and Activator of Transcription 3 (STAT3), TGF-β, DNA damage response (DDR)
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Senolytics - current state
Sekáč, Dávid ; Hodný, Zdeněk (advisor) ; Zima, Michal (referee)
Cellular senescence is a state of the permanent cell cycle arrest caused by different stresses or cell to cell fusion. Senescent cells, unlike naturally aged cells, exhibit a specific phenotype, referred to as senescence associated phenotype (SASP). It is characterized by the production of biologically active substances such as interleukins, chemoattractants or proteases that affect their surroundings. Long-term survival of these cells in the body is the cause of age-related diseases. Under normal circumstances, number of senescent cells is maintained in the body by the immune system. However, the age-related abrogation of immune system function per se (immunosenescence) contributes to accumulation of senescent cells in tissues and aging of organism. This work describes origin, positive and negative effects of cell senescence, elimination of senescent cells by the immune system and current state of development of new substances causing specific lysis (killing) of senescent cells (senolytics).
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The reaction on Marshall plan in chosen periodics of Czechoslovak press in 1947
Zima, Michal ; Suk, Pavel (advisor) ; Šírová, Tereza (referee)
The Marshall plan meant important step in recovering of European countries from World War II. The aim of this thesis is to describe the Marshall plan and put it into historical context of Czechoslovakian republic. The main emphasis is to analyse selected journals such as Rude pravo, Svobodne slovo and Pravo lidu. The author will focus on the reactions of journals on refusing the Marshall plan by Czechoslovakian government. The main question of the thesis is to answer what were the reactions on this process in journals and in society like.
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The role of mitochondrial energy metabolism in cell senescence
Zima, Michal ; Trnka, Jan (advisor) ; Pecinová, Alena (referee)
Cellular senescence represents status, when the cells cease to divide and remain in permanent cell cycle arrest. Senescence is considered to be an active response of the cell to various extrinsic and intrinsic types of stress such as certain oncogene activation, exposing to several cytokines or drugs and damaged and/or uncapped telomeres. Senescent cells are characterised by extensive modification of gene expression, flattened and enlargement of cellular body. Hypothetically, altered gene expression may lead also to increase of certain surface proteins expression. Such protein can be L1 cell adhesive molecule (L1CAM), which is expressed heterogeneously within the population. This Thesis describes current knowledge of cellular senescence, the mechanism, which may result in establishment of senescence phenotype, and also the characteristic markers of senescence. Thesis also puts together the heterogeneity of L1CAM expression in A375 senescent cells with oxygen consumption rate and extracellular acidification rate performed by Seahorse XFe24 metabolic analyser. Therefore, ells were sorted according to their levels of expressing L1CAM onto low and high L1CAM expressing subpopulations. Obtained data show potential correlation between the rate of L1CAM expression in A375 cells and the metabolic rate. Key...
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Changes in oxidative phosphorylation during development of cellular senescence
Zima, Michal ; Hodný, Zdeněk (advisor) ; Kašparová, Dita (referee)
Cellular senescence represents a state of permanent cell cycle arrest. It is considered to be an active response of the cell to various extrinsic and intrinsic types of stress, which are damaged and/or uncapped telomeres, activation of certain oncogenes, DNA damage and effects of several cytokines. This thesis describes current mechanisms which may result in establishment of senescence phenotype, putting those facts in association with changes in oxidative phosphorylation. In thesis are also mentioned features of senescence cells and their impact on the neighborhood. Special attention is focused on the role of reactive oxygen species in promotion of cellular senescence, mechanisms of their elevation, the role of NADPH oxidases and the inhibition of mitochondrial oxidative phosphorylation complexes by activity of cytokine signaling pathways STAT3 and TGFbeta. Key words: cellular senescence, reactive oxygen species, cytokines, mitochondria, oxidative phosphorylation chain, NADPH oxidases, Signal Transducer and Activator of Transcription 3 (STAT3), TGF-β, DNA damage response (DDR)
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Immigration and acculturation: review of factors affecting acculturation and specifications of social workers interventons
Zima, Michal ; Šiklová, Jiřina (advisor) ; Polo, Věra (referee)
Ciele: Cieľom práce bolo osvetliť proces adaptácie imigranta na novú situáciu a novú spoločnosť a poukázať na jednotlivé faktory, ktoré ovplyvňujú a formujú individuálne imigrantslé skúsenosti a pržívanie. V kontexte imigrantovej skúsenosti presídlenia sme taktiež poukázali na špecifiká intervencií sociálnych pracovníkov. Výskumná časť sa zameriava na priblíženie subjektívneho prežívania imigrantov, prehľad problémov, s ktorými sa počas akulturácie stretávajú a na celkové vykreslenie jednotlivých životných príbehov. Metódy: Výskum mal formu kvalitatívnej štúdie s použitím jednej prípadovej štúdie, ktorá predstavovala určitú škálu imigrantskej populácie. Základom prípadovej štúdie bolo pološtrukturované interview, zamerané na udalosti a pocity spojené s presídlením a následnou adaptáciou v novej spoločnosti. Medzi ďalšie zdroje informácií patrilo pozorovanie správania so zameraním sa na špecifické reakcie voči svojmu okoliu, monitorovanie tém, ktoré sa v našej interakcii javili ako konfliktné a aspektov, ktoré zostávali tabu, ako aj analýza súkromných a publikovaných písomných záznamov vlastného prežívania z tohoto obdobia. Výsledky: Výsledky analýzy prípadove štúdie poukázali na dôležitosť niektorých faktorov, ktoré mali a majú univerzálnu pôsobnosť na participanta samotného ako ja na proces jeho adptácie a...
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