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Conservation genetics of the grey wolf in Central Europe
Valentová, Kamila Anna ; Hulva, Pavel (advisor) ; Galov, Ana (referee)
Conservation genetics of the grey wolf in Czech Republic and adjacent regions is studied in the present thesis. Analyses of twenty-one microsatellite loci, one sex-determining amelogenin gene and mitochodrial control region were used to verify species determination, identify individuals and estimate relationships between them, analyse population structure and estimate demographic trends based on samples collected between 2014 and 2021. Genetic detection of red fox and dog samples incorrectly assigned to wolves illustrates the hurdles of field monitoring of grey wolf. Direct evidence for the occurrence of F1 hybrids was not found. Wolves from Bohemia showed lower values of allelic richness in comparison to the ones from Western Carpathians, probably as a consequence of recent expansion. Geographic distances between detection sites of identical individuals were relatively small or moderate in this study, suggesting regular movements of animals within their home ranges. Only two long-distance dispersal events exceeding 300 km were detected. Results of parental analysis provided evidence of pack distribution within the studied area. Most relationships were detected between wolves in the northern region of Czech Republic where the first recolonizing wolf pack in 2014 was registered. Within the studied...
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Abundance of and threats to the snow leopard in various regions of its occurrence
Valentová, Kamila ; Kindlmann, Pavel (advisor) ; Hulva, Pavel (referee)
Snow leopard (Panthera uncia) is an endangered species and its population size is steadily declining. This thesis attempts to introduce and analyse the main factors threating its survival with reference to each country of its occurrence: China, Bhutan, Nepal, Pakistan, Afghanistan, Tajikistan, Uzbekistan, Kyrgyzstan, Kazakhstan, Russia and Mongolia. To conserve the remaining snow leopard populations, it is necessary to detect its distribution in various areas. Here, recent data about its worldwide distribution are presented. Snow leopard has a very secretive lifestyle, which makes estimation of its abundance quite difficult. Therefore, I also present here an overview of methods, such as sign survey, capture-recapture, predator:prey biomass ratios, photo-capture rate and genetic analyses, used for estimation of abundance of snow leopard with reference to conducted studies. I discuss their advantages and disadvantages under different conditions. Key words: snow leopard, abundance, threats, worldwide distribution, conservation
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Conservation genetics of the grey wolf in Central Europe
Valentová, Kamila Anna ; Hulva, Pavel (advisor) ; Galov, Ana (referee)
Conservation genetics of the grey wolf in Czech Republic and adjacent regions is studied in the present thesis. Analyses of twenty-one microsatellite loci, one sex-determining amelogenin gene and mitochodrial control region were used to verify species determination, identify individuals and estimate relationships between them, analyse population structure and estimate demographic trends based on samples collected between 2014 and 2021. Genetic detection of red fox and dog samples incorrectly assigned to wolves illustrates the hurdles of field monitoring of grey wolf. Direct evidence for the occurrence of F1 hybrids was not found. Wolves from Bohemia showed lower values of allelic richness in comparison to the ones from Western Carpathians, probably as a consequence of recent expansion. Geographic distances between detection sites of identical individuals were relatively small or moderate in this study, suggesting regular movements of animals within their home ranges. Only two long-distance dispersal events exceeding 300 km were detected. Results of parental analysis provided evidence of pack distribution within the studied area. Most relationships were detected between wolves in the northern region of Czech Republic where the first recolonizing wolf pack in 2014 was registered. Within the studied...
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Abundance of and threats to the snow leopard in various regions of its occurrence
Valentová, Kamila ; Kindlmann, Pavel (advisor) ; Hulva, Pavel (referee)
Snow leopard (Panthera uncia) is an endangered species and its population size is steadily declining. This thesis attempts to introduce and analyse the main factors threating its survival with reference to each country of its occurrence: China, Bhutan, Nepal, Pakistan, Afghanistan, Tajikistan, Uzbekistan, Kyrgyzstan, Kazakhstan, Russia and Mongolia. To conserve the remaining snow leopard populations, it is necessary to detect its distribution in various areas. Here, recent data about its worldwide distribution are presented. Snow leopard has a very secretive lifestyle, which makes estimation of its abundance quite difficult. Therefore, I also present here an overview of methods, such as sign survey, capture-recapture, predator:prey biomass ratios, photo-capture rate and genetic analyses, used for estimation of abundance of snow leopard with reference to conducted studies. I discuss their advantages and disadvantages under different conditions. Key words: snow leopard, abundance, threats, worldwide distribution, conservation
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