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Automotive Alternator Innovation
Volek, Martin ; Hýsek, Roman (referee) ; Hájek, Vítězslav (advisor)
The work deals with the analytical electromagnetic design of automotive claw-pole alternator 14V/120A. This design is verified in program Maxwell with a RMxprt tool. The results of both calculations are compared. Designed alternator is also compared with the results measured in the laboratory on the alternators of similar type. Furthermore, there are analyzed individual power losses of machine and listed options of reducing these losses, which results in an increase in overall efficiency.
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Calibration of Digital Camera to Luminance Measurement
Volek, Martin ; Závada, Petr (referee) ; Baxant, Petr (advisor)
The thesis deals with the calibration of a digital camera for a purpose of luminance measurement. The aim is to create calibration approximation functions allowing the calculation of luminance. The first part analyses the basic light-technical parameters, as well as the principle of luminance measurement and the features of digital cameras for this purpose. An important issue, introduced in the present thesis, is to adapt a digital camera as a luminance meter. Furthermore, there is a description of the preparation of a site for the calibration, including necessary devices. The last part is dedicated to define the acquired calibration functions for the respective light sources and their mutual comparison.
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A role of demethylases from JARID 1 family proteins in forming splicing complex
Volek, Martin ; Stiborová, Marie (advisor) ; Martínek, Václav (referee)
Pre-mRNA splicing is a process in which introns are removed from pre-mRNA and exons are ligated. Recent studies confirm that 80 % of pre-mRNA splicing takes place cotranscriptionally, while the pre-mRNA is still attached to the RNA polymerase II. Therefore, pre-mRNA splicing occurs in close proximity to the chromatin, which might influence pre-mRNA splicing. Chromatin can be modified by several modifications. One chromatin modification is histone 3 lysin 4 tri-methylation (H3K4me3). This histone modification is found in the promoter-proximal region of genes, which are actively transcribed. The levels of this modification depend on the transcription activity RNA polymerase II. Indirect binding between H3K4me3 and the U2 snRNP particle via chromo- domain protein CHD1 has been proposed. After demethylation of H3K4me3, the role of this histone modification in forming of splicing complex can be possibly explained. Demethylation of H3K4me3 is catalyzed by the demethylase, JARID1C (Jmj domain, ARID domain). cDNA for JARID1C was cloned into a plasmid. After overexpression of JARID1C in HeLa cells, reduced levels of H3K4me3 were observed by Western blot and immunofluorescence. Differences in mobility of snRNP particles in HeLa cells were measured by fluorescence recovery after photobleaching (FRAP). For U2...
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Phenomenon smartphone
Skružný, Dominik ; Buchtík, Martin (advisor) ; Volek, Martin (referee)
The Bachelor thesis The Smartphone Phenomenon deals with the recent phenomenon of smartphones. Its aim is to place this phenomenon into selected parts of Castells' concept of network society. This work builds on Castells' concept and also tries to add some new facts to it. The author's attempt it to reach his goals through quantitative research done as a case study on heavy smartphone users. The author had done a series of semi-structured interviews and tried to obtain the data also through an experiment, in which the respondent's smartphones were taken away from him or her for one day. In the analytical part, the author analyses these interviews and experiments. The results of the analysis are categorized based on topics selected beforehand, in accordance with the theoretic background. The author then discusses the analytical and theoretical parts. This work emphasized the newly discovered aspects, which bring smartphones into the concept of network society.
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Clothes Make the Man: Dress as a Medium of Meanings
Märzová, Hana ; Šanderová, Jadwiga (advisor) ; Volek, Martin (referee)
In this thesis the meanings given by students to dress are examined. Then, these meanings are compared to those discussed in sociological literature dealing with dress and fashion. Dress is not only a protection of body against unpleasant weather but also a medium for meanings related to the wearer of the dress. Through a dress it is possible to express social status, social role, hobbies, opinions, membership in various groups and also lifestyle. Clothing and fashion are strongly associated with young people because the fashion is made for them and they contribute to its creation. For adolescents dress is a matter of particular importance because it enables them not only to express, but also to build their identity and thus reach adulthood. Dress has also an important role in our relations to other people. The analysis of interviews with students resulted in the finding that meanings given to dress by students are similar to those discussed in sociological literature. For interviewed students it is important to have a freedom to wear, what they want, because it enables them to be themselves. The findings from interviews rather contradict Simmel's assumption because a fashion is considered by interviewed students as uniform, while originality is located outside the fashion. Those students also...
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Influence of transcription regulatory elemets on pre-mRNA splicing
Volek, Martin ; Staněk, David (advisor) ; Malík, Radek (referee)
In the process of pre-mRNA splicing introns are removed from pre-mRNA and exons are joined together. Current studies show, that about 95 % of genes, which contain more than two exons, can undergo alternative splicing. In this process some exons are included in or excluded from the final mRNA. Majority of pre-mRNA splicing take place co- transcriptionaly at this time RNA polymerase II is still attached to pre-mRNA. Alternative splicing is complex process that takes place in a close proximity of DNA and histones that might modulate alternative splicing decisions. Futher studies have validated fibronectin gene (FN1) and his alternative exons EDA and EDB (extra domain A and B) as suitably model for studying alternative splicing. Study using FN1 minigene reporter system, which is composed from EDA exon and two surrounding introns and exons, has proved that insertion of transcription enhancer SV40 infront of promotor, the level of EDA inclusion is decreased. So far, has not been prooved if this mechanism can function in real genome context and if distal transcription elements can influence alternative splicing. In this study, we have predicted transcription enhancer for FN1 gene by using The Ensemble Regulatory Build and FANTOM 5. The predicted transcription enhancer, is located 23,5 kbp upstream of TSS...
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Influence of transcription regulatory elemets on pre-mRNA splicing
Volek, Martin ; Staněk, David (advisor) ; Malík, Radek (referee)
In the process of pre-mRNA splicing introns are removed from pre-mRNA and exons are joined together. Current studies show, that about 95 % of genes, which contain more than two exons, can undergo alternative splicing. In this process some exons are included in or excluded from the final mRNA. Majority of pre-mRNA splicing take place co- transcriptionaly at this time RNA polymerase II is still attached to pre-mRNA. Alternative splicing is complex process that takes place in a close proximity of DNA and histones that might modulate alternative splicing decisions. Futher studies have validated fibronectin gene (FN1) and his alternative exons EDA and EDB (extra domain A and B) as suitably model for studying alternative splicing. Study using FN1 minigene reporter system, which is composed from EDA exon and two surrounding introns and exons, has proved that insertion of transcription enhancer SV40 infront of promotor, the level of EDA inclusion is decreased. So far, has not been prooved if this mechanism can function in real genome context and if distal transcription elements can influence alternative splicing. In this study, we have predicted transcription enhancer for FN1 gene by using The Ensemble Regulatory Build and FANTOM 5. The predicted transcription enhancer, is located 23,5 kbp upstream of TSS...
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A role of demethylases from JARID 1 family proteins in forming splicing complex
Volek, Martin ; Stiborová, Marie (advisor) ; Martínek, Václav (referee)
Pre-mRNA splicing is a process in which introns are removed from pre-mRNA and exons are ligated. Recent studies confirm that 80 % of pre-mRNA splicing takes place cotranscriptionally, while the pre-mRNA is still attached to the RNA polymerase II. Therefore, pre-mRNA splicing occurs in close proximity to the chromatin, which might influence pre-mRNA splicing. Chromatin can be modified by several modifications. One chromatin modification is histone 3 lysin 4 tri-methylation (H3K4me3). This histone modification is found in the promoter-proximal region of genes, which are actively transcribed. The levels of this modification depend on the transcription activity RNA polymerase II. Indirect binding between H3K4me3 and the U2 snRNP particle via chromo- domain protein CHD1 has been proposed. After demethylation of H3K4me3, the role of this histone modification in forming of splicing complex can be possibly explained. Demethylation of H3K4me3 is catalyzed by the demethylase, JARID1C (Jmj domain, ARID domain). cDNA for JARID1C was cloned into a plasmid. After overexpression of JARID1C in HeLa cells, reduced levels of H3K4me3 were observed by Western blot and immunofluorescence. Differences in mobility of snRNP particles in HeLa cells were measured by fluorescence recovery after photobleaching (FRAP). For U2...
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