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Characterization of serine proteases of Schistosoma mansoni involved in interaction with host
Ulrychová, Lenka ; Horn, Martin (advisor) ; Schabussova, Irma (referee) ; Sojka, Daniel (referee)
Schistosomiasis is a serious parasitic disease, caused by blood flukes of the genus Schistosoma. It remains a global health problem in the 21st century, with more than 250 million people infected in 78 countries. Current therapy relies on the drugs praziquantel and oxamniquine, for which there are concerns of emerging drug resistance. Proteases of schistosomes are involved in critical steps of host-parasite interactions and are promising targets for the development of new therapeutic strategies against schistosomiasis. This work focuses on the characterization of Schistosoma mansoni serine proteases (SmSPs) and the determination of their role in the interaction with the human host using a variety of genomic, bioinformatic, RNA- and protein-based techniques. First, the major types of proteolytic activities secreted by the blood-dwelling developmental stages of S. mansoni were classified using functional proteomics. The analysis revealed the complexity of proteolytic activities secreted by the schistosome life stages parasitizing the human host. All stages secreted significant serine protease activities, and consequently their genes were retrieved from the genome database and annotated. Localization in adult worms determined by fluorescence in situ RNA hybridization revealed complex expression...
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Subcelulární lokalizace a úloha komplexu exocyst v savčích buňkách během cytokineze
Ulrychová, Lenka ; Hudeček, Jiří (advisor) ; Entlicher, Gustav (referee)
Cytokinesis is the last step of cell cycle when two individual daughter cells separate in process called abscission. This process involves various cellular membrane structures such as endoplasmic reticulum or trans-Golgi network. Moreover, recent investigation has also highlighted an important role of recycling endosomes. The membrane dynamics appear to be important during cell division especially for the formation of new plasma membrane between two daughter cells. Numerous studies suggest that cytokinesis is tightly linked with highly sophisticated transmembrane shuttle that is controlled by Ras-superfamily members such as Rab and Ral proteins. Moreover, during last years has also been revealed the involvement of tethering factors which mediate the fusion of intracellular vesicles with the target plasma membrane. The best known tethering factor is the evolutionary conserved exocyst complex found in all eukaryotic cells. This protein complex is composed of eight subunits (Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70 and Exo84) and was found to interact with members of Ras- superfamily suggesting its involvement in the regulation of cytokinesis. Although the exact mechanism remains shrouded in fog this work suppose the possible interactions among Ras- like proteins and exocyst members which may...
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Application of set-covering problem to fire district station Ovčáry
Ulrychová, Lenka ; Skočdopolová, Veronika (advisor) ; Kuncová, Martina (referee)
This thesis deals with the set-covering problem which belongs to distribution problems of the linear programming and in a practice can be found in many forms. The main goal of the standard set-covering problem is the construction of a new operating station which contains a degree of danger, but this thesis is focused on one operating station and examines the decision about its construction like a station of type JPO I, the problem contains so-called degrees of danger. Thesis is based on the real data provided by Fire Brigade of the Central region (Hasičský záchranný sbor Středočeského kraje). The theory of the distribution problems and optimization system MPL (which is the problem solved by) is presented in the first part. There a reader can also find a little information from the history of The Fire Brigade of the Czech Republic (HZS ČR). In the next part there is the description of modification for examining area coverage of the territory, where fire units are included instead of operating stations.
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