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Company´s Culture and its Influence on Customers
Tučková, Kristýna ; Kunertová, Helena (referee) ; Pokorný, Jiří (advisor)
This bachelor thesis deals with the corporate culture of ABC CYKLOSPORT s.r.o. and the promotion of the market. It is focused on maintaining customer satisfaction and the related increase in market share for the company. It is already established theoretical knowledge of the topic of corporate culture. In the second part there is a description and evaluation of questionnaire survey. These results constitute an established cornerstone for the design of measures that will lead to an increase in market share and streamline the work of employees.
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Influence of the citrulination of histon proteins on the expression of selected genes in myeloid cells
Tučková, Kristýna ; Dobeš,, Pavel (referee) ; Číž,, Milan (advisor)
Neutrophils are major cell type of innate immunity, that can eliminate pathogens by different mechanisms. One of these mechanisms is called NETosis, which leads to release of decondensed chromatin and citrullinated histone proteins. Citrullination is post-translational modification catalysed by peptidylarginine deiminase (PAD) and causing transformation of possitively charged arginin to neutral citrullin and can change expression of cytokine genes. Concetrations of pro-inflammatory cytokines (IL-8, TNF, IL-1) were measured after activation of PAD4 and induction of citrullination. Calcium ionophore was used to induce citrullinaton, Cl-amidine and TDFA were used as inhibitors. Production of cytokines was assessed by ELISA on protein level and by qPCR on mRNA level. It was found that induction of citrullination led to increased concentrations of IL-8 and IL-1. Elevated gene expression of IL-8 was confirmed on mRNA level. Both inhibitors were able to decrease level of histone H3 citrullination and IL-8 and IL-1 concentrations. Expression of TNF was not detected on protein and mRNA level.
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Increase the Competitivness of Firm
Tučková, Kristýna ; Žák, David (referee) ; Mikulec, Luděk (advisor)
This master´s thesis deals with problems competitiveness in the framework business of company ABC CYKLOSPORT s.r.o. and evaluates her contemporary position in the marketplace with sports goods. Results evaluation are important point by which unspools draft of measures largely focus on generation of competition advantage for company and increase number her customers.
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Influence of the citrulination of histon proteins on the expression of selected genes in myeloid cells
Tučková, Kristýna ; Dobeš,, Pavel (referee) ; Číž,, Milan (advisor)
Neutrophils are major cell type of innate immunity, that can eliminate pathogens by different mechanisms. One of these mechanisms is called NETosis, which leads to release of decondensed chromatin and citrullinated histone proteins. Citrullination is post-translational modification catalysed by peptidylarginine deiminase (PAD) and causing transformation of possitively charged arginin to neutral citrullin and can change expression of cytokine genes. Concetrations of pro-inflammatory cytokines (IL-8, TNF, IL-1) were measured after activation of PAD4 and induction of citrullination. Calcium ionophore was used to induce citrullinaton, Cl-amidine and TDFA were used as inhibitors. Production of cytokines was assessed by ELISA on protein level and by qPCR on mRNA level. It was found that induction of citrullination led to increased concentrations of IL-8 and IL-1. Elevated gene expression of IL-8 was confirmed on mRNA level. Both inhibitors were able to decrease level of histone H3 citrullination and IL-8 and IL-1 concentrations. Expression of TNF was not detected on protein and mRNA level.
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|
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Company´s Culture and its Influence on Customers
Tučková, Kristýna ; Kunertová, Helena (referee) ; Pokorný, Jiří (advisor)
This bachelor thesis deals with the corporate culture of ABC CYKLOSPORT s.r.o. and the promotion of the market. It is focused on maintaining customer satisfaction and the related increase in market share for the company. It is already established theoretical knowledge of the topic of corporate culture. In the second part there is a description and evaluation of questionnaire survey. These results constitute an established cornerstone for the design of measures that will lead to an increase in market share and streamline the work of employees.
|
|
|
Increase the Competitivness of Firm
Tučková, Kristýna ; Žák, David (referee) ; Mikulec, Luděk (advisor)
This master´s thesis deals with problems competitiveness in the framework business of company ABC CYKLOSPORT s.r.o. and evaluates her contemporary position in the marketplace with sports goods. Results evaluation are important point by which unspools draft of measures largely focus on generation of competition advantage for company and increase number her customers.
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