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PMD - polarization mode dispersion and its effects on data transport
Trávníček, Pavel ; Dostál, Otto (referee) ; Filka, Miloslav (advisor)
My graduation thesis is concerned with the effect of polarization mode dispersion on transmitted signal by optical line. It describes external and internal causes of origination of polarization mode dispersion. On the basis of these effects there is a differential group delay (DGD). It is the time delay in meantime within individual modes. DGD results in a time extension of impulsion and limitation of maximum bit rate. This thesis contains localization methods of part of gossamer filament with increased value of PMD by POTDR method. This method is based on the principium of back dispersion and its modifications SOP a DOP. Very important chapter of this thesis is the measurement of the polarization mode dispersion. There are described methods of PMD measurement. It is the interferometric and polarimetric method and the method of state of polarization. The major part of this thesis is about the reduction of polarization mode dispersion effect. This part is divided into two parts. First part is about the methods of PMD effect reduction and the second one is about the PMD compensation. The discrepancy between these two parts is the reduction way of PMD effect on transmitted channel. The compensation use the compensation components inserted into the transmission path. By PMD reduction methods we can solve the problems from the view of changing of fibre or all cable. For better understanding of polarization mode dispersion effect there are simulated 4 optical lanes of various parameters. The simulation output is the eye diagram which provides the view at results of transmission lane. In the final part there is the evaluation of measured parameters of optical lanes simulated in a laboratory.
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Evaluation of cytotype and morphological variability and hybridization rate in water-milfoils (Myriophyllum) in the Czech Republic and adjacent territories
Hrdinová, Magdalena ; Trávníček, Pavel (advisor) ; Koutecký, Petr (referee)
Myriophyllum L. (watermilfoil) belongs to one of the most invasive genus of the Northern Hemisphere. The three most agressive species, one of which is native to Europe (M. spicatum), are widespread throughout the majority of United states and two of them (M. aquaticum and M. heterophyllum) are becoming invasive in several Europaean countries as well. Therefore, Europaean populations of both native and invasive species, represents ideal place where to study processes which account for watermilfoils' invasivness. In this study, flow cytometry, morphometric analyses and germination experiments were employed to evaluate cytotype and morphological variability of Myriophyllum along with its potential to hybridize and propagate generatively. Five ploidy levels were detected in Europe, however no ploidy variability has been found within any species except for M. sibiricum (hexaploids and nonaploids) and one population of M. aquaticum (hexaploids and oktoploids). Flow cytometry proved to be useful tool for purposes of distinguishing species of different ploidy level and their potential hybrids. Nevertheless, the key species - M. sibiricum and M. spicatum - among which probably even more aggressive hybrids were recently identified, have similar hexaploid level. Thus flow cytometry cannot facilitate...
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Watermilfoils (Myriophyllum) in the central Europe
Hrdinová, Magdalena ; Trávníček, Pavel (advisor) ; Prančl, Jan (referee)
Genus Myriophyllum L. (watermilfoil) belongs to one of the most species-rich genera within Haloragaceae (Saxifragales). It includes three invasive species, which are of major concern - European M. spicatum L., North American M. heterophyllum MICHX. and South American M. aquaticum (VELL.) VERDC. All of them occur in Central Europe as well as native M. verticillatum L. and M. alterniflorum DC. lacking more significant invasive growth up to now. It is not only its invasivness that makes genus Myriophyllum so fascinating. It also displays extremely high phenotypic plasticity which is accompanied by determination difficulties. Moreover, recent research indicates that Myriophyllum tends to hybridize producing more invasive hybrids than parental taxa. Such traits pose a formidable challenge to scientists to deal with possible future invasive growth of non-indigenous Myriophyllum species in Central Europe. Therefore, thorough study of factors influencing evolution success of plants should be conducted. One of the key factors seems to be polyploidy. Research into polyploidy offers a new approach to watermilfoil issues. Additionally, according to preparatory study all native Central European Myriophyllum species can be easily distinguished by flow cytometry. That enables to study polyploidy in an...
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Genome size studies in plants - from intraspecific variation to ecological consequences
Lučanová, Magdalena ; Trávníček, Pavel (advisor) ; Leitch, Ilia (referee) ; Šmarda, Petr (referee)
Nuclear DNA content (genome size) is one of the basic characteristics of living organisms. In the Angiosperms, the range of genome size is 2,300-fold, which raises questions about the causes and consequences of this tremendous variation. This thesis deals with genome size in plants from the level of intraspecific homoploid variation, through intraspecific ploidy variation, to interspecies comparisons. On various study systems we investigated the dynamics and ranges of genome size variation, tried to reveal possible associations between genome size and selected biological traits, and assessed the extent to which differences in genome size are manifested at the ecological and evolutionary level. As a means of estimating genome size we applied flow cytometry (FCM). In Taraxacum stenocephalum we conducted a detailed study of its enormous genome size variation. We carried out crossings of parents with various genome sizes and compared these parental genome sizes with those of F1 offspring. We also attempted to reveal the association of genome size with various growth traits. In Galium valdepilosum and Arabidopsis arenosa we carried out an extensive flow-cytometric ploidy level screening and compared the distribution and ecological preferences of detected cytotypes. We studied the Andean genus...
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Ecological consequences of polyploidization in populations of the wetland plant Butomus umbellatus
Rydlo, Jan ; Fér, Tomáš (advisor) ; Trávníček, Pavel (referee)
This thesis aimed on comparison of environmental requirements and morphological and biological traits of diploid and triploid cytotypes of wetland plant flowering rush (Butomus umbellatus), occurring in Eastern Slovak Lowland in unique mixed populations, and to develop primers for variable microsatellite loci suitable for further genetic studies. In total, 72 populations of Butomus umbellatus were found. The analysis of relative genome size by the flow cytometry (FCM) technique revealed 34 exclusively triploid cytotype populations, 8 exclusively diploid populations and 30 mixed populations of Butomus umbellatus. In mixed populations, the numbers of diploid and triploid individuals are quite equivalent and the plants create combined compact communities. Analyses of environmental characteristics of sites with specific cytotypes (PCA, RDA, ANOVA) revealed no difference in site requirements of diploid and triploid Butomus umbellatus plants. Morphometric analyses (PCA, CDA) of traits of 36 diploid and 54 triploid plants confirmed differing morphology of both cytotypes occurring in the nature. Subsequent replanting of these plants into pots and cultivation further increased these differences. The ratio of width and length of internal perianthium is the most reliable parameter to distinguish diploid and...
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Sources of variation in the genus Anthoxanthum L.
Chumová, Zuzana ; Trávníček, Pavel (advisor) ; Mano Loureiro, Joao Carlos (referee) ; Kopecký, David (referee)
The genus Anthoxanthum (sweet vernal grass, Poaceae) represents a taxo- nomically intricate polyploid complex with a large phenotypic variation and still poorly resolved evolutionary relationships. The thesis deals with Anthoxanthum sect. Anthoxanthum in Europe, which comprises five traditionally recognized perennials (A. odoratum, diploid and tetraploid A. alpinum, A. maderense and A. amarum) and three annuals (A. gracile, A. aristatum and A. ovatum). Combination of different methods (DNA flow cytometry, sequencing of nrDNA ITS and two plastid regions, fluorescence and genomic in situ hybridization, climatic niche characterization and spatio-temporal modelling) was used to investigate ploidy and genome size variation of the genus across its European range, and to clarify the species origin and phylogeny of its members. Eight taxonomic groups that predominantly corresponded to traditionally recognized species were delimited on the basis of genome size values and phenotypic variation. While our data supported the merger of A. aristatum and A. ovatum, eastern Mediterranean populations traditionally referred to as diploid A. odoratum were shown to be cytologically distinct and may represent a new taxon. Genuine intraspecific variation in genome size was detected within the genus and the evidence for...
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Evolution of ascospore morphology and their dispersal in bryophilous Pezizales
Janošík, Lukáš ; Koukol, Ondřej (advisor) ; Trávníček, Pavel (referee)
Bryophilous Pezizales represent a species-rich group of fungi closely associated with bryophytes. Their ascospore morphology is highly variable and they strongly differ also in the genome size and ecology. They could thus represent an interesting model system for the research of evolution of ascospore morphology and their dispersal. The aims of my thesis were to test whether their genome size, number of nuclei in ascospores and ecology of host bryophyte influence their ascospore morphology and to experimentally test the effect of ascospore morphology on their active dispersal. I studied 52 species of bryophilous Pezizales. I reconstructed their phylogeny based on the sequences of three DNA regions, which I then used for the testing of relationships between individual variables using the phylogenetic generalized least squares. For the majority of species, I obtained measurements from morphometric analysis, genome size measurements using flow cytometry, and determined the number of nuclei in their ascospores using the fluorescent microscopy. I localised the infection apparatus and included also the ecological characteristics of the host bryophytes into the analyses. Using the experiments with horizontal ascospore discharge, I measured the distance of active ascospore ejection and recorded whether...
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