|
|
The polymorphism of sericin 2 gene in silkworm (Bombyx mori)
TYLLEROVÁ, Helena
In our study, we examined the polymorphism of gene Ser2 from domesticated silkmoth Bombyx mori and its closest wild relative B. mandarina. As a starting material for our work, we used the restriction map of allele C isolated from hybrid lineages 200 and 300 of European silkmoth B. mori (Michaille et al. 1990a). We also used the published sequence of allele D which was isolated from {\clq}qDaizo`` p50 strain of B. mori (Kludkiewicz et al 2009). Based on the published sequence, we designed PCR primers and performed sequence analysis of 4 different alleles of gene Ser2, including the almost complete genomic sequence of allele C (except for two short regions containing repetitive DNA). We then compared the sequences and found that the alleles differ significantly not only in intron lengths and the sizes of exon 9, but also in exon arrangements. While the allele C contains 12 exons (1, 2, 3, 4, 5, 6, 7, 8a, 9, 10, 11, 8b), the alelle D and other alleles analysed have 13 exons (1, 2, 3, 4, 5, 6, 7, 8, 9a, 10a, 9b, 10b, 11). Allele C showed very unique arrangement of exons, which suggested that it may represent an ancestral form of Ser2 gene. We analysed the arrangment of exons located at the 3´end of the Ser2 gene by PCR and electrophoresis in 70 available strains of B. mori and B. mandarina. We also tried to find a similar allele to the allele C and we amplified and sequenced 2 kb region from 55 Bombyx strains. Phyllogenetic analysis of gene Ser2 gene suggested that the C allele of now probably extinct European silkmoth hybrids 200 and 300 does not seem to be an ancestral form of Ser2 gene, but it rather seems to be the result of complex secondary rearrangements.
|
|
|
The analyse of polymorphisms of chicken
TYLLEROVÁ, Helena
Chicken growth hormone gene was examined in chosen strains from DOMINANT CZ breeding program - Rhode Island Red (RIR), New Hampshire (NH) and the final hybrids RIRxNH and NHxRIR. Polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) analyses reveal three MspI restriction sites of intron 1 region. These restriction sites give rise to six RFLP fragments {--} 540 bp, 390 bp, 270 bp, 240 bp, 150 bp, 120 bp. Based on restriction patterns, three alleles of growth hormone were determined. From a possible six genotypes, only four were found in all populations. The allele A1 is the most abundant in RIR strain (0.925), allele A3 is the most abundant in NH strain (0.550). The allele A1 was present in the most of the hybrids.
|
guest ::
