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Numerical image processing methods for rotating condenser microscope images processing
Týč, Matěj ; Martišek, Dalibor (referee) ; Druckmüller, Miloslav (advisor)
Tato práce popisuje vznik obrazu v transmisním světelném mikroskopu s proměnnou aperturou kondenzoru. Tento způsob pořizování obrazu je výhodný při pozorování tlustých vzorků, což jsou objekty, u kterých je v mikroskopickém měřítku významná výška. Klasické transmisní mikroskopy pro tento výzkum vhodné nejsou, protože výsledný obraz, který produkují, obsahuje patrné informace z velkého objemu vzorku mimo oblast, která je vyšetřovaná. Tento problém byl vyřešen po vynálezu konfokálního mikroskopu, který je ovšem daleko dražší a má i některé nevýhody navíc. Cílem je zpracovat obrazy pořízené pomocí kondenzoru s rotující aperturou tak, aby došlo k redukci podílu nežádoucí informace a obraz se tak stal "čistším". Tato metoda zpracování obrazu nemohla být použita v minulosti, protože tehdejší počítače neměly dostatečnou paměť a výkon. Na tuto metodu se dá nahlížet jako na převod množiny výstupů z mikroskopu s vylepšenou osvětlovací soustavou na odpovídající množinu výstupů konfokálního mikroskopu.
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Novel cancer biomarkers derived from quantitative phase imaging of biopsy cells
Plišková, Diana ; Týč, Matěj (referee) ; Kolářová, Jana (advisor)
The main objective of this work is the development of novel cancer biomarkers usable in personalized treatments. To understand why this issue is important, a brief description of cancer, including statistical results over the past years, is provided. The work also describes individual methods of light microscopy that can be used in cell analysis and subsequent image processing consisting of segmentation, tracking, feature extraction and classification. In this work, the main cell features, such as cell motility and shape, are presented. These features can be potential biomarkers in the treatment of cancer.
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Simulation of imaging in Reflected-light Holographic Microscope with optical aberrations
Effenberger, Adam ; Kvasnica, Lukáš (referee) ; Týč, Matěj (advisor)
The aim of this work is the simulation of a moving cell on a noisy backgroud. Experience gained during this work was used for correction of artificially created data. Subsequently it was used for the correction of real experiment a data. Spyder, open source cross-platform IDE for scientific programming in the Python language, was used for it. The simulation was successful, as well as the correction of artificially created data. The correction of real experiment data was only partly suscessfull . It gives us enough room for further improvement.
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Three-Dimensional Reconstruction of Image in Digital Holographic Microscopy
Týč, Matěj ; Karásek,, Vítězslav (referee) ; Martišek, Dalibor (referee) ; Chmelík, Radim (advisor)
This thesis deals with the topic of 3D image processing for digital holographic microscopy - numerical refocusing. This method allows to perform mathematically accurate defocus correction on image of a sample captured away from the sample plane and it was applicable only for images that were made made using coherent illumination source. It has been generalized to a form in which it is also applicable to devices that use incoherent (non-monochromatic or extended) illumination sources. Another presented achievement concerns hologram processing. The advanced hologram processing method enables obtaining more data mainly concerning precision of quantities from one hologram — normally, one would have to capture multiple holograms to get those. Both methods have been verified experimentally.
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Novel cancer biomarkers derived from quantitative phase imaging of biopsy cells
Plišková, Diana ; Týč, Matěj (referee) ; Kolářová, Jana (advisor)
The main objective of this work is the development of novel cancer biomarkers usable in personalized treatments. To understand why this issue is important, a brief description of cancer, including statistical results over the past years, is provided. The work also describes individual methods of light microscopy that can be used in cell analysis and subsequent image processing consisting of segmentation, tracking, feature extraction and classification. In this work, the main cell features, such as cell motility and shape, are presented. These features can be potential biomarkers in the treatment of cancer.
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Three-Dimensional Reconstruction of Image in Digital Holographic Microscopy
Týč, Matěj ; Karásek,, Vítězslav (referee) ; Martišek, Dalibor (referee) ; Chmelík, Radim (advisor)
This thesis deals with the topic of 3D image processing for digital holographic microscopy - numerical refocusing. This method allows to perform mathematically accurate defocus correction on image of a sample captured away from the sample plane and it was applicable only for images that were made made using coherent illumination source. It has been generalized to a form in which it is also applicable to devices that use incoherent (non-monochromatic or extended) illumination sources. Another presented achievement concerns hologram processing. The advanced hologram processing method enables obtaining more data mainly concerning precision of quantities from one hologram — normally, one would have to capture multiple holograms to get those. Both methods have been verified experimentally.
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Three-dimensional reconstruction of image in digital holographic microscopy
Týč, Matěj ; Chmelík, Radim (advisor)
This thesis deals with the topic of 3D image processing for digital holographic microscopy - numerical refocusing. This method allows to perform mathematically accurate defocus correction on image of a sample captured away from the sample plane and it was applicable only for images that were made made using coherent illumination source. It has been generalized to a form in which it is also applicable to devices that use incoherent (non-monochromatic or extended) illumination sources. Another presented achievement concerns hologram processing. The advanced hologram processing method enables obtaining more data mainly concerning precision of quantities from one hologram — normally, one would have to capture multiple holograms to get those. Both methods have been verified experimentally.
|
|
|
Numerical image processing methods for rotating condenser microscope images processing
Týč, Matěj ; Martišek, Dalibor (referee) ; Druckmüller, Miloslav (advisor)
Tato práce popisuje vznik obrazu v transmisním světelném mikroskopu s proměnnou aperturou kondenzoru. Tento způsob pořizování obrazu je výhodný při pozorování tlustých vzorků, což jsou objekty, u kterých je v mikroskopickém měřítku významná výška. Klasické transmisní mikroskopy pro tento výzkum vhodné nejsou, protože výsledný obraz, který produkují, obsahuje patrné informace z velkého objemu vzorku mimo oblast, která je vyšetřovaná. Tento problém byl vyřešen po vynálezu konfokálního mikroskopu, který je ovšem daleko dražší a má i některé nevýhody navíc. Cílem je zpracovat obrazy pořízené pomocí kondenzoru s rotující aperturou tak, aby došlo k redukci podílu nežádoucí informace a obraz se tak stal "čistším". Tato metoda zpracování obrazu nemohla být použita v minulosti, protože tehdejší počítače neměly dostatečnou paměť a výkon. Na tuto metodu se dá nahlížet jako na převod množiny výstupů z mikroskopu s vylepšenou osvětlovací soustavou na odpovídající množinu výstupů konfokálního mikroskopu.
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