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Study of Biological Properties of Hyaluronic Acid Derivatives
Svanovský, Evžen ; Lázníček, Milan (advisor) ; Tilšer, Ivan (referee) ; Mičuda, Stanislav (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of pharmacology and toxikology Candidate: Mgr. Evžen Svanovský Supervisor: Prof. PharmDr. Ing. Milan Lázníček, CSc. Title of Doctoral Thesis: Study of biological properties of hyaluronic acid derivatives This PhD thesis, is dealing with physiological properties of Hyaluronic Acid labelled with 111 In isotope. HA is naturally occurring Glycosaminoglycan within organisms with many important functions on the cellular and organ level, including physiological and pathological processes. Its character of physiologically occurring substance makes its tracing in the organism very difficult. Thus preparation of suitable derivative and repeatable procedure of labelling of this molecule became the most important point of the whole work. During the experimental part DTPA-HA molecule created by CPN Dolni Dobrouč has been labelled using well-known method used for labelling of DTPA molecules with 111 In. A reproducible procedure has been created where 0,4 M Acetate buffer pH 5,5 is mixed with an equal volume of DTPA-HA purified by gel filtration and required amount of isotope 111 In and the whole mixture is mixed for 0,5 h. This method was used for labelling of DTPA-HA molecules of three different molecular weights 10, 100 and 400kDa,...
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Study of Biological Properties of Hyaluronic Acid Derivatives
Svanovský, Evžen ; Lázníček, Milan (advisor) ; Tilšer, Ivan (referee) ; Mičuda, Stanislav (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of pharmacology and toxikology Candidate: Mgr. Evžen Svanovský Supervisor: Prof. PharmDr. Ing. Milan Lázníček, CSc. Title of Doctoral Thesis: Study of biological properties of hyaluronic acid derivatives This PhD thesis, is dealing with physiological properties of Hyaluronic Acid labelled with 111 In isotope. HA is naturally occurring Glycosaminoglycan within organisms with many important functions on the cellular and organ level, including physiological and pathological processes. Its character of physiologically occurring substance makes its tracing in the organism very difficult. Thus preparation of suitable derivative and repeatable procedure of labelling of this molecule became the most important point of the whole work. During the experimental part DTPA-HA molecule created by CPN Dolni Dobrouč has been labelled using well-known method used for labelling of DTPA molecules with 111 In. A reproducible procedure has been created where 0,4 M Acetate buffer pH 5,5 is mixed with an equal volume of DTPA-HA purified by gel filtration and required amount of isotope 111 In and the whole mixture is mixed for 0,5 h. This method was used for labelling of DTPA-HA molecules of three different molecular weights 10, 100 and 400kDa,...
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