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Abuse of Children and adolescent
Sutrová, Veronika ; Koťa, Jaroslav (advisor) ; Klapálková, Veronika (referee)
The topic of the bachelor thesis is the issue of abuse of children and adolescents. At the beginning of the theoretical part, the work is focused on the definition of the term abuse and its various forms, namely mental and physical abuse, neglect, bullying and sexual abuse. The child abuse and neglect syndrome is also mentioned here. In the next chapter, the topic is the victims and perpetrators of abuse. More precisely description of typical figures and characteristics of people, which are involved in most cases. The conclusion of the theoretical part is targeted at the topic of prevention of abuse and assistance to victims of abuse and similar acts. The practical part of the bachelor's thesis contains three casuistries, which show specifically how the process of abuse takes place and what consequences it leaves. Key words children, adolescents, abuse, misuse, physical abuse, mental abuse, abuse prevention, syndrome CAN
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Optimization of new active surfaces based on plasmonic nanoparticle assemblies for SERS, SERRS and surface-modified luminescence studies of selected molecules
Sutrová, Veronika
Two types of 3-dimensional (3D) Ag nanosponge aggregates were prepared and tested as samples for surface-enhanced Raman scattering (SERS) and as active surfaces for surface-enhanced luminescence. 3D Ag nanosponge aggregates were assembled from 2D fused fractal aggregates (D = 1.87 ± 0.02) prepared by modification of Ag nanoparticle (NP) hydrosol resulting from the reduction of AgNO3 by NH2OH∙HCl. For SERS measurements, 3D Ag nanosponge aggregates with incorporated [Ru(bpy)3]2+ cations and chloride anions were prepared and overlayed by a thin layer of aqueous phase. For SEL measurements, the 3D Ag nanosponge aggregates were assembled from fused fractal aggregates of chloride-modified Ag NPs. After preparation the active surface was overlayed by a 1×10-5 M aqueous solution of [Ru(bpy)3]2+ . The SERRS (1×10-15 M) and SER(R)S (1×10-14 M) limits of detection of [Ru(bpy)3]2+ determined at 445 and 532 nm excitations, respectively, correspond to the single molecule level of the complex detection. Its achievement is attributed to a large electromagnetic mechanism enhancement experienced by [Ru(bpy)3]2+ incorporated in "hot spots", an efficient localization of "hot spots" in the 3D aggregate to the focus of the laser beam in micro-Raman spectral measurements and to a molecular resonance contribution to the...
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Optimization of new active surfaces based on plasmonic nanoparticle assemblies for SERS, SERRS and surface-modified luminescence studies of selected molecules
Sutrová, Veronika
Two types of 3-dimensional (3D) Ag nanosponge aggregates were prepared and tested as samples for surface-enhanced Raman scattering (SERS) and as active surfaces for surface-enhanced luminescence. 3D Ag nanosponge aggregates were assembled from 2D fused fractal aggregates (D = 1.87 ± 0.02) prepared by modification of Ag nanoparticle (NP) hydrosol resulting from the reduction of AgNO3 by NH2OH∙HCl. For SERS measurements, 3D Ag nanosponge aggregates with incorporated [Ru(bpy)3]2+ cations and chloride anions were prepared and overlayed by a thin layer of aqueous phase. For SEL measurements, the 3D Ag nanosponge aggregates were assembled from fused fractal aggregates of chloride-modified Ag NPs. After preparation the active surface was overlayed by a 1×10-5 M aqueous solution of [Ru(bpy)3]2+ . The SERRS (1×10-15 M) and SER(R)S (1×10-14 M) limits of detection of [Ru(bpy)3]2+ determined at 445 and 532 nm excitations, respectively, correspond to the single molecule level of the complex detection. Its achievement is attributed to a large electromagnetic mechanism enhancement experienced by [Ru(bpy)3]2+ incorporated in "hot spots", an efficient localization of "hot spots" in the 3D aggregate to the focus of the laser beam in micro-Raman spectral measurements and to a molecular resonance contribution to the...
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Optimization of new active surfaces based on plasmonic nanoparticle assemblies for SERS, SERRS and surface-modified luminescence studies of selected molecules
Sutrová, Veronika ; Vlčková, Blanka (advisor) ; Procházka, Marek (referee)
Two types of 3-dimensional (3D) Ag nanosponge aggregates were prepared and tested as samples for surface-enhanced Raman scattering (SERS) and as active surfaces for surface- enhanced luminescence. 3D Ag nanosponge aggregates were assembled from 2D fused fractal aggregates (D = 1.87 ± 0.02) prepared by modification of Ag nanoparticle (NP) hydrosol resulting from the reduction of AgNO3 by NH2OH·HCl. For SERS measurements, 3D Ag nanosponge aggregates with incorporated [Ru(bpy)3]2+ cations and chloride anions were prepared and overlayed by a thin layer of aqueous phase. For SEL measurements, the 3D Ag nanosponge aggregates were assembled from fused fractal aggregates of chloride- modified Ag NPs. After preparation the active surface was overlayed by a 1×10-5 M aqueous solution of [Ru(bpy)3]2+ . The SERRS (1×10-15 M) and SER(R)S (1×10-14 M) limits of detection of [Ru(bpy)3]2+ determined at 445 and 532 nm excitations, respectively, correspond to the single molecule level of the complex detection. Its achievement is attributed to a large electromagnetic mechanism enhancement experienced by [Ru(bpy)3]2+ incorporated in "hot spots", an efficient localization of "hot spots" in the 3D aggregate to the focus of the laser beam in micro-Raman spectral measurements and to a molecular resonance contribution to the...
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Development and testing of new types of active surfaces for investigation of surface-modified optical processes of biologically important molecules
Sutrová, Veronika ; Vlčková, Blanka (advisor) ; Procházka, Marek (referee)
Hydrosols of silver nanoparticles (AgNPs) prepared by reduction of AgNO3 by either NaBH4 (BH-AgNP hydrosol), or NH2OH·HCl (HA-AgNP hydrosol) modified by the addition of HCl were characterized by SPE spectra (SPE = surface plasmon extinction) and transmission electron microscopy, and their SERS (Surface-enhanced Raman Scattering) activity was tested by measuring the SERS or SERRS (Surface-enhanced Resonance Raman Scattering) spectra of 2,2'-bipyridine (bpy) and 5,10,15,20-Tetrakis(1-methyl-4-pyridinio)-21H,23H-porphine (H2TMPyP) testing adsorbates and by determining of their SERS and SERRS spectral detection limits at λexc = 532 nm. For both types of AgNP hydrosols, it was found that the addition of HCl and the addition of testing adsorbate leads to an intergrowth of AgNPs and to the formation of their compact aggregates, in combination with the formation of new Ag(0) adsorption sites. The limit of detection in a macrosampling setup was 1·10-9 M in the system HA-AgNP hydrosol /HCl/bpy and 1·10-5 M in the system BH AgNP hydrosol/HCl/bpy and, in both causes the Ag(0)-bpy surface complex was detected. About one order of magnitude lower detection limit of bpy in the system with HA-AgNP hydrosol is caused by the efficient intergrowth of AgNP in HA-hydrosol resulting into an efficient formation of "hot...
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