|
|
HPLC EVALUATION OG FUMAGILLIN IN THE MEDICINE
Strýčková, Kristýna ; Mokrý, Milan (advisor) ; Sochor, Jaroslav (referee)
HPLC EVALUATION OG FUMAGILLIN IN THE MEDICINE Rigorous thesis Mgr. Kristýna Strýčková Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control In this rigorous thesis is described analytic evaluation of fumagillin by using HPCL and stated the amount of fumagillin in the medicine. The measurment was implemented on chromatographic colony in size 3 x 150 mm I.D. with the Seladon SGX NH2 filling, 7μm. There was used the mobile phase composed of methanol: amonium acteate (aqueous solution 0,005 mol/l, v/v )in the ratio of 60:40, at the flow rate of 1ml/min and pressure of 2,5-2,6 MPa. The detection was performed at 335 nm using the UV detektor. The samples were spraeyd in cubage 20 μ/l. The international standard method was drawn up for determination of fumagillin in medicine. The most suitable sample from the selected conditions was nimesulid. Concerning validation parameters were verified linearity, accuracy, robustness, selection and detector limit. Further there was examined the stability in conditions of room temperature and the temperature od 2-8˚C. It was determined 0,0238 g of fumagillin in 1 g of medicine that is stated as 95,2% of neccessary amount.
|
|
|
Analytical evaluation of drugs using chromatographic methods III.
Strýčková, Kristýna ; Mokrý, Milan (advisor) ; Sochor, Jaroslav (referee)
Analytical evaluation of drugs using Chromatographic Methods III. Thesis Kristýna Strýčková Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control The thesis draws up the optimum chromatographic conditions for the HPLC analysis of fumagillin in honey and the procedure of the isolation of the drug from honey. The optimum results were provided by the analysis on the Tessek column with the Separon SGX NH2 filling, while using the mobile phase composed of methanol : ammonium acetate (aqueous solution 0.005 mol/l, v/v) in the ratio of 60:40, at a flow rate of 1 ml/min and pressure of 25 to 26 bar. The detection was performed at 335 nm using the UV detector. The internal standard method was drawn up for the determination of fumagillin in honey. Nimesulid suited the selected conditions most. As far as the validation parameters are concerned, the linearity was verified on the basis of calibration curve.
|
|
|
HPLC EVALUATION OG FUMAGILLIN IN THE MEDICINE
Strýčková, Kristýna ; Mokrý, Milan (advisor) ; Sochor, Jaroslav (referee)
HPLC EVALUATION OG FUMAGILLIN IN THE MEDICINE Rigorous thesis Mgr. Kristýna Strýčková Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control In this rigorous thesis is described analytic evaluation of fumagillin by using HPCL and stated the amount of fumagillin in the medicine. The measurment was implemented on chromatographic colony in size 3 x 150 mm I.D. with the Seladon SGX NH2 filling, 7μm. There was used the mobile phase composed of methanol: amonium acteate (aqueous solution 0,005 mol/l, v/v )in the ratio of 60:40, at the flow rate of 1ml/min and pressure of 2,5-2,6 MPa. The detection was performed at 335 nm using the UV detektor. The samples were spraeyd in cubage 20 μ/l. The international standard method was drawn up for determination of fumagillin in medicine. The most suitable sample from the selected conditions was nimesulid. Concerning validation parameters were verified linearity, accuracy, robustness, selection and detector limit. Further there was examined the stability in conditions of room temperature and the temperature od 2-8˚C. It was determined 0,0238 g of fumagillin in 1 g of medicine that is stated as 95,2% of neccessary amount.
|
guest ::
RSS feed.