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Presence and function of glycosidas in the reproductive tract of mammals
Slavík, Martin ; Postlerová, Pavla (advisor) ; Tuzhilkin, Roman (referee)
Glycosidases are enzymes that hydrolytically cleave polysaccharide structures and have fundamental importance above all for processing the glycocalyx on the surface of the sperm during the fertilization process, so that the sperm can finally successfully interact with the zona pellucida and fuse with the oocyte membrane. The bachelor thesis investigates the activities of several important glycosidases (β-galactosidases, α-galactosidases, α-mannosidases and neuraminidases) in mixed samples of reproductive fluids, specifically it is epididymal fluid, seminal plasma and oviductal fluid, originating from the domestic pig. Another sample in which the activity was measured of the aforementioned enzymes was ejaculated sperm. The activities of the given enzymes were determined using fluorescent substrates in slightly acidic and neutral pH. In addition to determining specific activity of individual enzymes, this bachelor's thesis was enriched with qualitative information regarding glycoproteins in male and female reproductive fluids and ejaculated sperm lysate. Glycoproteins are the dominant component of the sperm glycocalyx and many of them also represent the corresponding naturally occurring substrates for the above-mentioned glycosidases. Based on electrophoretic separation followed by staining, the...
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Use of uranine-probe method to determine evaporation rate from porous media
Pohan, Tomáš ; Slavík, Martin (advisor) ; Mareš, Jakub (referee)
Evaporation from porous media affects many natural processes. In my work, I focused on the second evaporation phase, calculating the evaporation rate using Fick's law based on air temperature and air humidity above the surface, the water-vapor permeability of the porous material, and the evaporation front depth below the surface. To determine this depth, I used the uranine-probe method using sodium fluorescein. The work aimed to calculate the evaporation rate using Fick's law and values of the evaporation front depth determined by the uranine-probe, for 6 sandy soil samples and 3 types of rocks. A sub-goal was to verify the applicability of the uranine-probe for determining the depth of the evaporation front in sandy soil and in 12 different rock samples. Results show successful determination of evaporation front depth for both sand and rocks. The uranine-probe method's accuracy averaged 2.0 mm for sand. The difference in the values determined by the uranine-probe method compared to another, independent method (detection of the evaporation front from the color change of the fluorescein solution) in the sand was on average 1.7 mm, but I did not study this for the rock samples. All evaporation experiments confirmed that the evaporation rate, both measured and calculated, decrease with increasing depth...
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Contaminant transport in karst environment and tracer tests in karst system near Turnov
Malechová, Kateřina ; Slavík, Martin (advisor) ; Weiss, Tomáš (referee)
Karst areas are an important source of drinking water but they are at significant risk of contamination. The theoretical part of the thesis focuses on different types of contaminants, their sources and their ways of transport in karst areas. Due to the high velocity of water flow in karst areas, most contaminants are transported rapidly and spread over long distances. Therefore, it is necessary to actively address the issue of contamination in karst environments, focusing mainly on contamination prevention and water flow studies. In the practical part of the thesis, a quantitative tracer test was carried out in the karst system near Turnov using NaCl as a tracer. The measurements confirmed a high water flow velocity of several kilometers per day and the dominance of advection as the main transport process. These results are consistent with previous research. The connection between the sinking stream Šálení smyslů and the Slapy and Bezednice springs was also reconfirmed, with a total recovery of around 50%. This implies that water from the Šálení smyslů most likely flows to other, yet unknown, locations. No communication with the Bartošova pec cave or with the springs used as a source for the local brewery has been demonstrated, so the question remains where all the water from the sinking stream...
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Measuring the retention curve of rocks: the example of sandstone from the Jizera formation
Fataciune, Sotir ; Slavík, Martin (advisor) ; Ondovčin, Tomáš (referee)
Retention curve describes the ability of an unsaturated porous medium to retain water at different suction pressures. It thus plays an important role in providing information on the movement and occurrence of water in the vadose zone. While the determination of the retention curve for soil is common, it is far less common in the case of rocks. The aim of this bachelor thesis is to determine the retention curve of sandstone from the Jestrebicka jehla in Kokorinsko using the tensiometer - weight method. A partial aim is to compare this curve with two previous measurements on the same sandstone: i) measurements by student A. Studencová, using the tensiometer - weight method, and ii) measurements made by the laboratory of Research Institute for Soil and Water Conservation, according to a slightly modified method of CSN ISO 11274. There were notable differences between the retention curves. The retention curve according to the CSN for a given suction pressure showed lower moisture values, which is probably due to insufficient saturation of the sample and air entrapment in the rock pores. Relatively large differences in the retention curve between two independent tensiometer - weight method measurements were also found, which are probably due to insufficient contact between the tensiometer and the porous...
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Analysis of pitch perception depending on the spectrum
Slávik, Martin ; Indrák, Michal (referee) ; Jirásek, Ondřej (advisor)
This thesis is dealing with the research of the influence of individual harmonic components on the perception of sound height. As a part of the work has been made an internet questionnaire on a web address www.analyzavyšek.9e.cz, where individual respondents completed listening test compound of 46 samples. This experiment is based on semantic differential method. Individual samples are compound of sine wave – tone and examined sample, which respondents evaluates on three bipolar scales. The samples were either modelled by additive synthesis or modified sampled sounds of instruments. Individual samples were divided to four major groups and other minor groups for clarity. The purpose was to find which parts, combinations and harmonic components of sound and tone can affect perception of tone height.
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The Study of Production's Operation Management
Slavík, Martin ; Škaroupka, Jan (referee) ; Jurová, Marie (advisor)
In my diploma thesis I have focused on the optimization of ‘Production Operational Management’ in terms of change management and work organization in the department of thermo case and pre-production within the company Automotive Lighting, s.r.o. which is a producer of automobile light technique. The intention of the first part is to produce general characteristics of the company. The analysis of the current stage results from the production system and the organizational structure particularly in the pre-production department of thermo cases. Based on this analysis I have designed the proposal so that it consists of a new organizational structure and a new work organization with a view to customer satisfaction within a demanding product while taking into consideration quality and time. In the final part of this thesis I have looked at the contribution and feasibility conditions of the output proposal.
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Measurement of Stream Flow Rate Using Salt Dilution Method
Burdych, Marek ; Ondovčin, Tomáš (advisor) ; Slavík, Martin (referee)
Tracer dilution method for measuring flow rate in surface streams is the main topic of the bachelor's thesis. The first part contains a summary of the most important flow measuring techniques for surface streams, including current meter, float method, FlowTracker, ADCP, volumetric method, and computing techniques based on channel cross-section data. Large part is dedicated to tracer dilution method. The findings of field measurements of flow made on the Lo- děnice River using the salt dilution approach are detailed in the second section. Five conductometers were used to measure the water conductivity at three di- fferent sites. Conductometry was performed simultaneously in two different distances from injection site. Throughout the experiment, a sixth conductome- ter recorded the background conductivity value as a standard. The predicted flow values are compared to those acquired from nearby hyd- rometric station of the Czech Hydrometeorological Institute (CHMI) and from observations made using Flowtracker. Diferences in measured flow rate were interpreted as tracer losses. These losses were estimated. Keywords: hydrology, surface water, water stream, tracer dilution, conducti- vity, tracer
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The influence of evapotranspiration on the groundwater of floodplain forests: Libický luh
Vašková, Hana ; Weiss, Tomáš (advisor) ; Slavík, Martin (referee)
The recherche part of this bachelor thesis deals with evapotranspiration from floodplain forests and its influence on the depth of the groundwater level and mineralization of groundwater. As floodplain forests in the Czech Republic are represented only in smaller fragments, there is a mention of the effect of mineralization on plants. The thesis describes the principle and use of stable isotopes in hydrogeology and the comprehensive characteristics of the area of interest of the experimental part, the Libický luh National Nature Reserve. The experimental part includes the observation of the level, conductivity, temperature, pH, and determination of the isotopic composition of the underground and surface water in Libický luh, where high mineralization of the groundwater was previously detected. The thesis discusses the effect of evapotranspiration on the content of dissolved substances in the water in Libický luh as a possible cause of this phenomenon. It was found that as the temperature rises, the groundwater level drops, and that mineralization remains relatively constant throughout the year. The drop in the groundwater level is evidently caused by intensive evapotranspiration from the floodplain forest during the growing season. However, longer time series of conductivity or hydrochemical...
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Selected issues in hydrology and hydrogeology of the Trebon Basin
Nymsa, Zdeněk ; Slavík, Martin (advisor) ; Nedvěd, Jakub (referee)
In the last few decades, the average temperatures in the Czech Republic have been increasing, which has led to increase in evapotranspiration. Previous studies have shown that increases in evapotranspiration during the summer months can lead to significant reductions in flows and even to drying up of small watercourses. Additionally, snow cover has also been on the decline in recent years, resulting in no flow enhancement in spring months and therefore lower soil moisture problems ensue. Due to the possible decrease in precipitation and/or increase in evapotranspiration, there is also the risk of less groundwater recharge in the future. The aim of my thesis is to verify the correlation between several selected parameters in the Trebon Basin, namely the relationship between evapotranspiration, precipitation and baseflow and groundwater levels in boreholes. A sub-objective is to verify the long- term evolution of these parameters in the Trebon Basin. Atmospheric precipitation and its variability during the year have not changed since the 1960s, while the magnitude of potential evapotranspiration has statistically significantly increased over this time and its variability during the year has decreased. The difference between atmospheric precipitation and potential evapotranspiration has been...
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Hydrogeological character of selected hardrock environments and groundwater recharge in populated areas
Krupař, Josef ; Bruthans, Jiří (advisor) ; Slavík, Martin (referee)
The purpose of this thesis is to adress the hydrogeology of hard-rock environment and groundwater recharge in urban areas. The theoretical part describes hydrogeology character of granite and ophiolite rock environment with emphasis on features such as transmissivity, storativity and hydraulic conductivity that corresponds to the fissuring of the rock environment. It is clear that fault tectonics is the most important factor that influence the hydraulic conductivity. Storativity, on the other hand, is most affected by fissured systems. Ophiolites are a more significant water supply aquifer than granites in terms of storativity. This thesis also describes groundwater recharge in urban areas and not only from precipitation but also through surface water reservoirs, leaks from pipeline network and by import of water from surrounding watershed. Practical part compares values of precipitation from 2013 to 2022 from four ČHMU stations. It compares PET (Potential evapotranspiration), API (antecendant precipitation index) values and their difference. Subsequently, in the practical part, the normalized relative groundwater recharge from the measured values of the well level is compared with the flow rate at all monitored locations and it is evaluated whether the time course of the recharge according to the...
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