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Determination of glutathion in plant samples using capillary electrophoresis
Skála, Michael ; Křížek, Tomáš (advisor) ; Kubíčková, Anna (referee)
This work is based on a previously published method for determination of both glutathione forms by capillary zone electrophoresis equipped with UV spectrometric detector. Theoretical part described biological and biochemical functions of glutathione as a molecule. It mentioned historically used methods for glutathione determination, their accuracy, precision and reliability and focused on electrophoretic methods, especially capillary zone electrophoresis. Basic principles of capillary zone electrophoresis were described, followed by an overview of approaches to treatment of different types of samples, with special focus on plant samples. At the end, method this work was based on was described. The experimental part describes the development and optimization of this method. Firstly, optimum chemical composition and ionic strength of background electrolyte were determined - two electrolytes were compared, with NaCl and without NaCl - no significant differences were observed, so NaCl was excluded from BGE. Next, optimum concentration of hexadimethrine bromide was determined. The effect of HDB concentration on electroosmotic flow was investigated. Calibration curve for different concentrations of analytes in water was constructed and repeatability, LOD and LOQ of the method were determined....
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Metadata and accessing informations on the internet
Škála, Michael ; Sklenák, Vilém (advisor) ; Vojíř, Stanislav (referee)
The aim of this bachelors thesis is firstly introduce reader with metadata by its theoretical side. Then make approach with examples of systems of my choise, which currently works with metadata, namely Wikipedia and digital libraries. In the next chapter I describe how web search engines works and also you can find here some of the most well-known representatives of search engines. The last part is aimed on web pages optimization for search engines with SEO and SEM techniques, which helps with making web pages more visible and potentional increase in traffic of web pages. The result of this work is introducing reader with basic functions of metadata and describe practical systems, in which you can find them. Also it suppose to familiarize the reader with functioning of web search engines and with optimization principles of web pages in which you can also use them and approach how metadata effect optimization.
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