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Preparation of Cosmetic Product Based on Microbial Extracts
Reichertová, Klára ; Trachtová, Štěpánka (referee) ; Hudečková, Helena (advisor)
This thesis is focused on the preparation of a cosmetic product based on microbial extracts. The thesis is divided into two main parts, theoretical and practical. The theoretical part describes the types of cultured microorganisms, microbial metabolites, legislation and safety of cosmetic products, composition of human skin and last but not least the applied analyses. The experimental part deals with the use of waste products of the food industry, specifically coffee grounds as a substrate for culture media. Furthermore, the cultivation is studied with a focus on the gain of metabolites useful in the cosmetic industry, the analysis of these metabolites by GC/FID and HPLC/PDA. The work addresses the preparation of microbial extracts, their testing including determination of polyphenol and flavonoid content and characterization of antioxidant activity and antimicrobial activity tested on bacterial cultures of Esterichia coli and Staphylococus epidermidis. The application of the analysed extracts and their application to the final product is also addressed. The last chapter discussed in this thesis to test the final product using Visioscope PC–35, instrument and performing sensory analysis. The cultured strains were Phaffia rhodozyma CCY 77-1-1, Cystophilobasidium macerans CCY 10-1-2, Rhodosporidium toruloides CCY 062-002-004 Chlorella vulgaris CCALA 924. The biomass obtained by cocultivation of Phaffia rhodozyma CCY 77-1-1 and Chlorella vulgaris CCALA 924 as well as the biomass of these two microorganisms cultured separately were used for the preparation of microbial extracts.
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Application of microbial oils in cosmetics
Reichertová, Klára ; Uhlířová, Renata (referee) ; Němcová, Andrea (advisor)
This bachelor thesis is focused on the application of microbial oil in a cosmetic product. Eight strains of yeasts of the genus Metschnikowia were examined, namely Metschnikowia pulcherrima 145, Metschnikowia pulcherrima 147, Metschnikowia pulcherrima 149, Metschnikowia andauensis 129, Metschnikowia andauensis 1241, Metschnikowia sinensis 1244, Metschnikowia zizyphicola 1247 and Metschnikowia shanxiensis 1250 for production of microbial oil. Substrates made of technical hemp (hemp flour, hydrolyzate and a mixture of leaves and flowers) were used for cultivation. The cultivation was performed at reduced temperature with the advantage of lipid production. The composition and ratio of fatty acids were determined by gas chromatography According to these resulst was choosen the best lipid producer and the most abundant substrate. This was the strain Metschnikowia zizyphicola 1247 and the substrate of hemp flour. At the end of the practical part was prepared a cream, into which the microbial oil was mixed. Finally, an evaluation of the oil with regard to its use in cosmetics and a comparison with vegetable oil with the same fatty acid profile are given.
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Application of microbial oils in cosmetics
Reichertová, Klára ; Uhlířová, Renata (referee) ; Němcová, Andrea (advisor)
This bachelor thesis is focused on the application of microbial oil in a cosmetic product. Eight strains of yeasts of the genus Metschnikowia were examined, namely Metschnikowia pulcherrima 145, Metschnikowia pulcherrima 147, Metschnikowia pulcherrima 149, Metschnikowia andauensis 129, Metschnikowia andauensis 1241, Metschnikowia sinensis 1244, Metschnikowia zizyphicola 1247 and Metschnikowia shanxiensis 1250 for production of microbial oil. Substrates made of technical hemp (hemp flour, hydrolyzate and a mixture of leaves and flowers) were used for cultivation. The cultivation was performed at reduced temperature with the advantage of lipid production. The composition and ratio of fatty acids were determined by gas chromatography According to these resulst was choosen the best lipid producer and the most abundant substrate. This was the strain Metschnikowia zizyphicola 1247 and the substrate of hemp flour. At the end of the practical part was prepared a cream, into which the microbial oil was mixed. Finally, an evaluation of the oil with regard to its use in cosmetics and a comparison with vegetable oil with the same fatty acid profile are given.
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