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National Repository of Grey Literature

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	Measurement of electric potential on biological objects Rúbal, Radek ; Friedl, Martin (referee) ; Klusáček, Stanislav (advisor) Resource kit for measuring voltage sources with high impedance was designed. This resource kit is suitable for measuring electric potentials on biological objects, especially on plants. Based on the design, workplace controlled from PC that allows automatic measurement was implemented. Workplace functionality was experimentally verified. Experiments led to use of electrodes that minimize interfering electrochemical voltage. Electric potential changes were recorded not only in connection with different substrates but also with the change of light intensity. Detailed record
	Realisation of method for fluorescence lifetime and spectral changes evaluation using advanced confocal microscopy techniques Rúbal, Radek ; Janoušek, Oto (referee) ; Čmiel, Vratislav (advisor) Content is focused on fluorescence lifetime imaging techniques. Fluorescence lifetime is computed from data acquired with using of Leica TCS SP8X confocal microscope sequential scanning. Algorithms and software for the computation, imaging and analysis of fluorescence lifetime is presented. Software is allowing both 2D and 3D imaging of fluorescence lifetime. Techniques are used for fluorescence lifetime imaging of mesenchymal cells and fibroblasts tainted with SPIO-Rhodamin complex. Detailed record
	Measurement of electric potential on biological objects Rúbal, Radek ; Friedl, Martin (referee) ; Klusáček, Stanislav (advisor) Resource kit for measuring voltage sources with high impedance was designed. This resource kit is suitable for measuring electric potentials on biological objects, especially on plants. Based on the design, workplace controlled from PC that allows automatic measurement was implemented. Workplace functionality was experimentally verified. Experiments led to use of electrodes that minimize interfering electrochemical voltage. Electric potential changes were recorded not only in connection with different substrates but also with the change of light intensity. Detailed record
	Realisation of method for fluorescence lifetime and spectral changes evaluation using advanced confocal microscopy techniques Rúbal, Radek ; Janoušek, Oto (referee) ; Čmiel, Vratislav (advisor) Content is focused on fluorescence lifetime imaging techniques. Fluorescence lifetime is computed from data acquired with using of Leica TCS SP8X confocal microscope sequential scanning. Algorithms and software for the computation, imaging and analysis of fluorescence lifetime is presented. Software is allowing both 2D and 3D imaging of fluorescence lifetime. Techniques are used for fluorescence lifetime imaging of mesenchymal cells and fibroblasts tainted with SPIO-Rhodamin complex. Detailed record

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