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Impact of nitrogen fertilization and drought on gliadins content in winter wheat
Odstrčilová, Eva ; Obruča, Stanislav (referee) ; Hrstka, Miroslav (advisor)
In this diploma thesis the impacts of both nitrogen fertilization and drought on the content of proteins of the gliadin fraction in case of a winter wheat variety Tiguan were observed. Selected samples were cultivated at two locations in a total duration of two years. Two different conditions were selected: one without the nitrogen fertilization (0 kg N/ ha) and the other with the nitrogen fertilization in 140 kg N/ ha concentration. Second observed factor was the drought which was ensured by roofs above the crops and their cultivation in common climatic environment. Gliadin fraction was separated by the A-PAGE method and the protein quantification carried out by a computer densitometry. Such nitrogen fertilization caused a significant increase of gliadins, especially -1,2 and -5 fractions. Stress induced by the drought caused an increase of gliadin content compared to the control sample, particularly in case of - and - fractions. The most important factor influencing the gliadin content in grains was therefore the nitrogen fertilization in a dry environment. The observed increase of gliadin content was lower in case of the sample which was fertilized in a humid environment than in case of the sample which was fertilized in the dry one.
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Pectin - its properties and industrial applications
Odstrčilová, Eva ; Vránová, Dana (referee) ; Omelková, Jiřina (advisor)
This thesis deals with pectins substances in food processing and pharmaceutical industry. Pectin is very using component in food processing products, mainly due to jelly and thickening ability. The thesis is dedicated plant cell wall, where is located pectin including other material component of plant cell wall. Further it is described structure of pectin, including his production and use in industry. The last part of the thesis is dedicated enzymatic degradation of plants origin materials. Pectin is very using, but its structure and biosynthesis is still not fully explored.
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Impact of nitrogen fertilization and drought on gliadins content in winter wheat
Odstrčilová, Eva ; Obruča, Stanislav (referee) ; Hrstka, Miroslav (advisor)
In this diploma thesis the impacts of both nitrogen fertilization and drought on the content of proteins of the gliadin fraction in case of a winter wheat variety Tiguan were observed. Selected samples were cultivated at two locations in a total duration of two years. Two different conditions were selected: one without the nitrogen fertilization (0 kg N/ ha) and the other with the nitrogen fertilization in 140 kg N/ ha concentration. Second observed factor was the drought which was ensured by roofs above the crops and their cultivation in common climatic environment. Gliadin fraction was separated by the A-PAGE method and the protein quantification carried out by a computer densitometry. Such nitrogen fertilization caused a significant increase of gliadins, especially -1,2 and -5 fractions. Stress induced by the drought caused an increase of gliadin content compared to the control sample, particularly in case of - and - fractions. The most important factor influencing the gliadin content in grains was therefore the nitrogen fertilization in a dry environment. The observed increase of gliadin content was lower in case of the sample which was fertilized in a humid environment than in case of the sample which was fertilized in the dry one.
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Pectin - its properties and industrial applications
Odstrčilová, Eva ; Vránová, Dana (referee) ; Omelková, Jiřina (advisor)
This thesis deals with pectins substances in food processing and pharmaceutical industry. Pectin is very using component in food processing products, mainly due to jelly and thickening ability. The thesis is dedicated plant cell wall, where is located pectin including other material component of plant cell wall. Further it is described structure of pectin, including his production and use in industry. The last part of the thesis is dedicated enzymatic degradation of plants origin materials. Pectin is very using, but its structure and biosynthesis is still not fully explored.
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