|
|
Evaluation of new "fused-core" analytical columns in UHPLC system - a comparison with BEH UHPLC analytical columns
Nežádalová, Jitka ; Nováková, Lucie (advisor) ; Kujovská Krčmová, Lenka (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical chemistry Candidate: Jitka Nežádalová Supervisor: PharmDr. Lucie Nováková, Ph.D. Title of Diploma thesis: Evaluation of properties of new "fused - core" analytical columns in UHPLC system and a comparison with the BEH UPLC analytical columns This thesis was focused on testing properties of new analytical columns filled with porous shell particles (so-called fused-core) in UHPLC system and a comparison with the BEH UPLC analytical column. The following analytical columns were tested: Kinetex PFP particle size of 1,7 µm and 2,6 µm (Phenomenex) and Ascentis Express Phenyl-Hexyl particle size of 2,7 µm (Sigma- Aldrich), which are columns with porous shell particles and Acquity BEH Phenyl column with totally porous particles size of 1,7 µm. To compare the properties of the columns, 7 estrogen steroids were used using PDA detector at a wavelength of 280 nm for detection. Measurements were carried out using isocratic elution. The Mobile phase consisted of ultrapure water and acetonitrile. Columns were evaluated in terms of efficiency, peak symmetry, resolution and the back pressure, which was generated on the columns. The efficiency of the columns was expressed as a number of theoretical plates and by van Deemter...
|
|
|
Evaluation of new "fused-core" analytical columns in UHPLC system - a comparison with BEH UHPLC analytical columns
Nežádalová, Jitka ; Nováková, Lucie (advisor) ; Kujovská Krčmová, Lenka (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical chemistry Candidate: Jitka Nežádalová Supervisor: PharmDr. Lucie Nováková, Ph.D. Title of Diploma thesis: Evaluation of properties of new "fused - core" analytical columns in UHPLC system and a comparison with the BEH UPLC analytical columns This thesis was focused on testing properties of new analytical columns filled with porous shell particles (so-called fused-core) in UHPLC system and a comparison with the BEH UPLC analytical column. The following analytical columns were tested: Kinetex PFP particle size of 1,7 µm and 2,6 µm (Phenomenex) and Ascentis Express Phenyl-Hexyl particle size of 2,7 µm (Sigma- Aldrich), which are columns with porous shell particles and Acquity BEH Phenyl column with totally porous particles size of 1,7 µm. To compare the properties of the columns, 7 estrogen steroids were used using PDA detector at a wavelength of 280 nm for detection. Measurements were carried out using isocratic elution. The Mobile phase consisted of ultrapure water and acetonitrile. Columns were evaluated in terms of efficiency, peak symmetry, resolution and the back pressure, which was generated on the columns. The efficiency of the columns was expressed as a number of theoretical plates and by van Deemter...
|
guest ::
