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Functional analysis of endothelial dysfunction in mice aorta following high-fat diet administration
Martínková, Adéla ; Němečková, Ivana (advisor) ; Vitverová, Barbora (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Supervisor: RNDr. Ivana Němečková, Ph.D. Author: Adéla Martínková Title of Thesis: Functional analysis of endothelial dysfunction in mice aorta following high-fat diet administration Background and aim: Endothelial dysfunction is associated with the impairment of vasodilatory response of vessel wall mediated by nitric oxide which is produced by endothelial cells under physiological conditions. Endothelial dysfunction can be induced by high fat diet (HFD) feeding in experimental animals. The aim of this diploma thesis was to determine the effect of HFD on the functional parameters of aorta, especially the effect of the vasodilatory factor acetylcholine on endothelial cells of the thoracic part of the mouse aorta. Methods: The 7 months old C57BL/6J male mice were fed high fat diet containing 42 % kcal fat for 12 weeks to induce endothelial dysfunction in the aorta. The control group was fed a standard laboratory diet during the whole experiment. Functional parameters of aorta were assessed with wire myograph 620M. Results: At the end of the experiment, the weight of the animals was statistically significantly lower in the HFD group compared to the control group of mice fed the standard...
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Functional analysis of endothelial dysfunction in mice aorta following high-fat diet administration
Martínková, Adéla ; Němečková, Ivana (advisor) ; Vitverová, Barbora (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Supervisor: RNDr. Ivana Němečková, Ph.D. Author: Adéla Martínková Title of Thesis: Functional analysis of endothelial dysfunction in mice aorta following high-fat diet administration Background and aim: Endothelial dysfunction is associated with the impairment of vasodilatory response of vessel wall mediated by nitric oxide which is produced by endothelial cells under physiological conditions. Endothelial dysfunction can be induced by high fat diet (HFD) feeding in experimental animals. The aim of this diploma thesis was to determine the effect of HFD on the functional parameters of aorta, especially the effect of the vasodilatory factor acetylcholine on endothelial cells of the thoracic part of the mouse aorta. Methods: The 7 months old C57BL/6J male mice were fed high fat diet containing 42 % kcal fat for 12 weeks to induce endothelial dysfunction in the aorta. The control group was fed a standard laboratory diet during the whole experiment. Functional parameters of aorta were assessed with wire myograph 620M. Results: At the end of the experiment, the weight of the animals was statistically significantly lower in the HFD group compared to the control group of mice fed the standard...
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European Arrest Warrant in the Light of Principle of Mutual Trust and Protection of Fundamental Rights
Martínková, Adéla ; Scheu, Harald Christian (advisor) ; Navrátil, Petr (referee)
European Arrest Warrant in the Light of Principle of Mutual Trust and Protection of Fundamental Rights Abstract European arrest warrant (EAW) is one of key instruments of mutual recognition in criminal matters. Essential elements of its fonctioning differentiate it from a traditional system of extradition and allow the system of EAW to be simpler and more efficient. EAW owns its efficiency to principle of mutual trust, which lays at its core. However, mutual trust cannot be blind and one of its limits is protection of fundamental rights. This thesis aims to find answers for questions: what are the fonctions of both principles within EAW, how are they balanced and what development has taken place so far in this matter. In order to do so, attention is brought first to the development of cooperation in criminal matters. Second chapter focuses on principle of mutual trust, which is essential for mutual recognition and EAW, but lacks a clear definition within EU law. Following chapter introduces protection of fundamental rights in EU and shows how it affects level of mutual trust between states. In the case of EAW, lack of protection of fundamental rights causes for states to be less willing to surrender a person to other member states. Level of fundamental rights protection affects the level of mutual trust and...
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