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Participatory creation of fictional world on the SCP Foundation forum
Manďák, Michal ; Bílek, Petr (advisor) ; Krásová, Eva (referee)
This thesis explores the SCP Foundation internet forum, its community and work. It consists of three parts. The first part offers a description of the forum's functioning and of its content. It mainly describes its participatory model, which allows all of its users to contribute to the forum's content and to review already published texts. It also describes the strict formal requirements of the SCP articles, which make up the majority of the forum's content. The second part concerns itself with the forum's cohesion. Even though the forum's authors should be in theory working on a single fictional world, their works may contradict each other. This work explores the convergent forces inside the forum from three perspectives: the perspective of fictional worlds generated by the texts, the perspective of canon formation, and the perspective of the constitution of meaning in the texts. It suggests a conceptual cluster model for the forum's set of fictional worlds, describes the role of authorities and generic users in the formation of a dynamic canon, shows the central motivic conflict of an institution and the supernatural, and describes the possibilities of digital text. The third part of this thesis offers an analysis of one of the forum's authorial continuities, on which it demonstrates conclusions...
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Use of RT-PCR in stress reaction studies
MANDÁK, Michal
Although the share of rapeseed (Brassica napus) in comparison with other oil crops on the world market continues to rise, its cultivation potential ,as with many other crops, is still limited due to its abiotic cold stress sensitivity. Therefore, the theoretical part of this thesis deals with the issue of plant stress and the description of the RT-qPCR method in the context of its suitability for quantification of changes that occur during cold stress. In the practical part of this thesis, an analysis of gene expression in selected dehydrin gene ERD10 was performed including description of sampling from plants, method of RNA isolation, reverse transcription into cDNA and own RT-qPCR. The research sample consisted of two rapeseed varieties Cadeli and Navajo subjected to cold stress at (2°C). The initial assumption was that there would be a noticeable difference in the expression level of the gene ERD10 between these varieties, depending on the ability of these plants to withstand cold stress. Although some samples were not measured, the interpretation of the data confirmed original assumption. During the first 6 days of cold stress, the Navajo variety had a significantly higher expression of this gene than the Cadeli variety. Based on the association between ERD10 expression and cold resistance, it should be possible to use ERD10 as one of the markers for the selection of cold resistant genotypes.
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