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National Repository of Grey Literature

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	Dehydroepiandrosterone (DHEA) ? mechanisms of biological effect. Machovská, Jana ; Herink, Josef (advisor) ; Nachtigal, Petr (referee) Detailed record
	Dehydroepiandrosterone (DHEA) ? mechanisms of biological effect. Machovská, Jana ; Nachtigal, Petr (referee) ; Herink, Josef (advisor) Detailed record
	Influence of salmonella lipopolysachcaride on gene trascription and secretion of interferon gamma. Machovská, Jana ; Celer, Vladimír (referee) ; Jílek, Petr (advisor) Candidate: Bc. Jana Machovská Title of diploma thesis: Influence of salmonella lipopolysaccharide on gene transcription and secretion of interferon gamma Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Aim: The aim of this diploma thesis is to evaluate the influence of a type of salmonella lipopolysaccharide on the activation of gene transcription and production of gamma interferon (IFN-γ) as a key cytokine of immune response to intracellular bacterial pathogens. The study was performed on infectious model of a gnotobiotic pig infected with gram- negative bacteria Salmonella enterica serovar Typhimurium strain LT2 or its rfaL- mutant. Methods: 1) Four groups of piglets were created: germ-free as a control group, conventional piglets, piglets infected with LT2 strain, piglets infected with rfaL- mutant 2) Determination of bacterial CFU in blood and intestinal lavage by cultivation on bacteriological media 3) Isolation of a total RNA from ileum, spectrophotometric quantification and estimation of its purity (A280/A260) 4) Reverse transcription into cDNA 5) Real-time PCR with LNA probes 6) Normalization and relativization of real-time PCR data 7) Estimation of cytokines in blood and intestinal lavage by ELISA. Results: 1) A bacterial... Detailed record

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